Ludwigia yellow vein virus
Average proteome isoelectric point is 7.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q4GZJ5|Q4GZJ5_9GEMI Protein V2 OS=Ludwigia yellow vein virus OX=325111 GN=av2 PE=3 SV=1
MM1 pKa = 7.95 WDD3 pKa = 3.57 PLEE6 pKa = 5.16 HH7 pKa = 6.71 EE8 pKa = 4.95 FPEE11 pKa = 4.38 TLHH14 pKa = 6.71 GFRR17 pKa = 11.84 CMLAIKK23 pKa = 9.78 YY24 pKa = 7.92 LQTVADD30 pKa = 4.84 TYY32 pKa = 11.87 APDD35 pKa = 3.48 TVGYY39 pKa = 10.58 DD40 pKa = 4.16 LIRR43 pKa = 11.84 DD44 pKa = 4.52 LISIVRR50 pKa = 11.84 AGNYY54 pKa = 9.38 VEE56 pKa = 3.79 ATRR59 pKa = 11.84 RR60 pKa = 11.84 YY61 pKa = 8.47 SHH63 pKa = 7.11 FNTRR67 pKa = 11.84 LQSTSPVEE75 pKa = 3.85 LRR77 pKa = 11.84 FPRR80 pKa = 11.84 NEE82 pKa = 3.96 PCCCPHH88 pKa = 7.15 CPRR91 pKa = 11.84 HH92 pKa = 5.74 NPKK95 pKa = 10.37 EE96 pKa = 4.09 CLDD99 pKa = 3.9 VQAHH103 pKa = 5.56 VSQAQTVQNVQKK115 pKa = 10.49 PP116 pKa = 3.5
Molecular weight: 13.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.064
IPC2_protein 6.122
IPC_protein 6.249
Toseland 6.453
ProMoST 6.707
Dawson 6.634
Bjellqvist 6.576
Wikipedia 6.649
Rodwell 6.634
Grimsley 6.547
Solomon 6.649
Lehninger 6.649
Nozaki 6.898
DTASelect 7.059
Thurlkill 7.088
EMBOSS 7.088
Sillero 7.044
Patrickios 3.427
IPC_peptide 6.664
IPC2_peptide 6.985
IPC2.peptide.svr19 6.847
Protein with the highest isoelectric point:
>tr|Q4GZJ1|Q4GZJ1_9GEMI Replication-associated protein OS=Ludwigia yellow vein virus OX=325111 GN=ac1 PE=3 SV=1
MM1 pKa = 7.04 GHH3 pKa = 7.2 CISMPSSNSKK13 pKa = 10.06 VKK15 pKa = 10.36 PNSGTPGILTLRR27 pKa = 11.84 IPTPPHH33 pKa = 5.25 NSIRR37 pKa = 11.84 TSKK40 pKa = 10.35 EE41 pKa = 3.31 PSPVLMSSPISRR53 pKa = 11.84 RR54 pKa = 11.84 TVITSTGVFFKK65 pKa = 10.31 STEE68 pKa = 3.97 DD69 pKa = 3.44 LLEE72 pKa = 4.31 EE73 pKa = 4.11 VSRR76 pKa = 11.84 RR77 pKa = 11.84 LMMQQQKK84 pKa = 9.68 RR85 pKa = 3.58
Molecular weight: 9.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.282
IPC2_protein 9.648
IPC_protein 10.423
Toseland 11.14
ProMoST 11.477
Dawson 11.169
Bjellqvist 10.921
Wikipedia 11.433
Rodwell 11.359
Grimsley 11.184
Solomon 11.433
Lehninger 11.374
Nozaki 11.125
DTASelect 10.921
Thurlkill 11.111
EMBOSS 11.564
Sillero 11.111
Patrickios 11.14
IPC_peptide 11.433
IPC2_peptide 10.072
IPC2.peptide.svr19 9.198
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1038
85
311
173.0
19.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.684 ± 1.012
2.505 ± 0.467
4.913 ± 0.587
4.528 ± 0.541
4.528 ± 0.501
5.202 ± 0.408
3.854 ± 0.281
6.166 ± 0.847
5.78 ± 0.733
6.936 ± 0.492
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.119 ± 0.629
5.106 ± 0.44
6.647 ± 0.918
4.721 ± 0.677
7.225 ± 0.815
6.647 ± 1.31
6.166 ± 1.152
6.069 ± 0.601
1.445 ± 0.204
3.757 ± 0.66
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here