Arthrobacter phage StarLord
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 87 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5J6TWQ9|A0A5J6TWQ9_9CAUD Uncharacterized protein OS=Arthrobacter phage StarLord OX=2599836 GN=9 PE=4 SV=1
MM1 pKa = 6.72 TQQAAITTVDD11 pKa = 3.16 NPFDD15 pKa = 3.57 VFTQFDD21 pKa = 3.7 EE22 pKa = 4.27 WNAWDD27 pKa = 4.59 LEE29 pKa = 4.43 HH30 pKa = 7.66 GYY32 pKa = 7.93 GTCAYY37 pKa = 8.99 LARR40 pKa = 11.84 VVRR43 pKa = 11.84 SSDD46 pKa = 3.29 EE47 pKa = 4.14 LSIADD52 pKa = 3.29 QDD54 pKa = 3.98 LALEE58 pKa = 4.06 YY59 pKa = 10.85 GIDD62 pKa = 3.56 EE63 pKa = 5.2 IIRR66 pKa = 11.84 EE67 pKa = 4.36 DD68 pKa = 3.97 VLGLYY73 pKa = 10.38 KK74 pKa = 10.34 KK75 pKa = 10.61 VLADD79 pKa = 3.79 KK80 pKa = 10.53 EE81 pKa = 4.33 DD82 pKa = 3.63 VSTSEE87 pKa = 4.09 SSEE90 pKa = 3.9
Molecular weight: 10.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.094
IPC2_protein 3.884
IPC_protein 3.834
Toseland 3.63
ProMoST 3.961
Dawson 3.821
Bjellqvist 4.024
Wikipedia 3.745
Rodwell 3.656
Grimsley 3.541
Solomon 3.795
Lehninger 3.757
Nozaki 3.935
DTASelect 4.139
Thurlkill 3.681
EMBOSS 3.757
Sillero 3.948
Patrickios 3.223
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.876
Protein with the highest isoelectric point:
>tr|A0A5J6U140|A0A5J6U140_9CAUD Uncharacterized protein OS=Arthrobacter phage StarLord OX=2599836 GN=65 PE=4 SV=1
MM1 pKa = 7.5 SAPKK5 pKa = 9.87 PSEE8 pKa = 4.21 VNTKK12 pKa = 8.89 PRR14 pKa = 11.84 KK15 pKa = 8.87 EE16 pKa = 3.6 RR17 pKa = 11.84 SAPVRR22 pKa = 11.84 NPAFRR27 pKa = 11.84 EE28 pKa = 4.13 SEE30 pKa = 4.16 PLKK33 pKa = 10.5 DD34 pKa = 3.55 LQRR37 pKa = 11.84 RR38 pKa = 11.84 LHH40 pKa = 6.56 AEE42 pKa = 3.66 ARR44 pKa = 11.84 NKK46 pKa = 9.87 KK47 pKa = 9.78
Molecular weight: 5.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.279
IPC2_protein 9.692
IPC_protein 10.452
Toseland 11.184
ProMoST 11.169
Dawson 11.213
Bjellqvist 10.965
Wikipedia 11.477
Rodwell 11.433
Grimsley 11.228
Solomon 11.462
Lehninger 11.418
Nozaki 11.155
DTASelect 10.965
Thurlkill 11.155
EMBOSS 11.608
Sillero 11.155
Patrickios 11.199
IPC_peptide 11.477
IPC2_peptide 9.823
IPC2.peptide.svr19 9.024
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
87
0
87
17087
41
1516
196.4
21.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.872 ± 0.508
0.603 ± 0.099
6.063 ± 0.332
6.619 ± 0.383
3.681 ± 0.185
7.491 ± 0.42
1.785 ± 0.193
5.934 ± 0.25
6.373 ± 0.384
8.053 ± 0.41
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.815 ± 0.209
4.512 ± 0.191
4.167 ± 0.23
3.318 ± 0.168
5.22 ± 0.302
5.969 ± 0.28
6.397 ± 0.334
6.97 ± 0.203
1.726 ± 0.174
3.43 ± 0.348
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here