Lactobacillus phage JNU_P2
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6M3BE75|A0A6M3BE75_9CAUD Uncharacterized protein OS=Lactobacillus phage JNU_P2 OX=2686382 PE=4 SV=1
MM1 pKa = 7.64 TNPLLAQIPAIFPLMAEE18 pKa = 4.16 SFEE21 pKa = 4.19 LLRR24 pKa = 11.84 VGEE27 pKa = 4.53 SPSDD31 pKa = 3.58 DD32 pKa = 3.39 ATGGFSGPIEE42 pKa = 4.48 DD43 pKa = 3.96 TSKK46 pKa = 11.23 LEE48 pKa = 4.53 PIGTICEE55 pKa = 4.0 PMIPEE60 pKa = 4.4 SKK62 pKa = 10.2 AQTMQMGEE70 pKa = 4.06 GGSFTNYY77 pKa = 9.19 AYY79 pKa = 10.54 QWFSMTKK86 pKa = 8.14 YY87 pKa = 10.42 PKK89 pKa = 9.29 GTLVRR94 pKa = 11.84 YY95 pKa = 9.88 DD96 pKa = 4.13 DD97 pKa = 4.27 LVLEE101 pKa = 4.31 IVEE104 pKa = 4.25 VEE106 pKa = 4.58 PYY108 pKa = 9.37 WSQGGFAIYY117 pKa = 10.72 YY118 pKa = 5.93 MQNRR122 pKa = 11.84 SDD124 pKa = 3.74 TDD126 pKa = 3.6 VQSS129 pKa = 3.33
Molecular weight: 14.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.845
IPC2_protein 4.101
IPC_protein 3.999
Toseland 3.821
ProMoST 4.126
Dawson 3.961
Bjellqvist 4.113
Wikipedia 3.846
Rodwell 3.834
Grimsley 3.745
Solomon 3.948
Lehninger 3.897
Nozaki 4.075
DTASelect 4.215
Thurlkill 3.859
EMBOSS 3.859
Sillero 4.113
Patrickios 1.914
IPC_peptide 3.948
IPC2_peptide 4.088
IPC2.peptide.svr19 4.002
Protein with the highest isoelectric point:
>tr|A0A6M3BE82|A0A6M3BE82_9CAUD Uncharacterized protein OS=Lactobacillus phage JNU_P2 OX=2686382 PE=4 SV=1
MM1 pKa = 8.39 VLMILFFSTAIYY13 pKa = 10.07 LISLVYY19 pKa = 8.85 ITRR22 pKa = 11.84 EE23 pKa = 4.01 TLRR26 pKa = 11.84 KK27 pKa = 9.5 SHH29 pKa = 5.96 VAVSNRR35 pKa = 11.84 SALVVLLILIGWNLKK50 pKa = 9.78 ILFRR54 pKa = 11.84 SRR56 pKa = 11.84 FDD58 pKa = 2.9 KK59 pKa = 10.99 KK60 pKa = 10.65 RR61 pKa = 11.84 FYY63 pKa = 11.29 KK64 pKa = 10.5 LLKK67 pKa = 10.37 SFLFEE72 pKa = 4.04 YY73 pKa = 9.22 PISVIVLGEE82 pKa = 4.24 IIFSDD87 pKa = 3.39 AKK89 pKa = 10.03 KK90 pKa = 10.01 HH91 pKa = 6.22 SKK93 pKa = 10.29 ISDD96 pKa = 3.19 KK97 pKa = 11.27 SVIHH101 pKa = 5.43 VSISISGLSFNGLKK115 pKa = 9.94 RR116 pKa = 11.84 RR117 pKa = 11.84 KK118 pKa = 9.27 NKK120 pKa = 9.86 QNLPIYY126 pKa = 9.37 EE127 pKa = 4.13 GLIAEE132 pKa = 4.79 LL133 pKa = 4.12
Molecular weight: 15.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.22
IPC2_protein 9.75
IPC_protein 9.926
Toseland 10.555
ProMoST 10.116
Dawson 10.672
Bjellqvist 10.292
Wikipedia 10.804
Rodwell 11.228
Grimsley 10.716
Solomon 10.701
Lehninger 10.672
Nozaki 10.511
DTASelect 10.292
Thurlkill 10.54
EMBOSS 10.921
Sillero 10.57
Patrickios 10.95
IPC_peptide 10.701
IPC2_peptide 8.799
IPC2.peptide.svr19 8.745
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
71
0
71
14449
37
1929
203.5
22.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.333 ± 0.426
0.353 ± 0.063
6.776 ± 0.467
5.301 ± 0.376
3.523 ± 0.192
7.018 ± 0.691
2.139 ± 0.163
5.551 ± 0.271
8.118 ± 0.555
7.544 ± 0.238
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.748 ± 0.148
5.198 ± 0.222
3.433 ± 0.213
4.014 ± 0.238
4.353 ± 0.256
7.198 ± 0.331
6.686 ± 0.344
6.547 ± 0.264
1.308 ± 0.116
3.834 ± 0.257
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here