Lactobacillus phage JNU_P2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3BE75|A0A6M3BE75_9CAUD Uncharacterized protein OS=Lactobacillus phage JNU_P2 OX=2686382 PE=4 SV=1
MM1 pKa = 7.64TNPLLAQIPAIFPLMAEE18 pKa = 4.16SFEE21 pKa = 4.19LLRR24 pKa = 11.84VGEE27 pKa = 4.53SPSDD31 pKa = 3.58DD32 pKa = 3.39ATGGFSGPIEE42 pKa = 4.48DD43 pKa = 3.96TSKK46 pKa = 11.23LEE48 pKa = 4.53PIGTICEE55 pKa = 4.0PMIPEE60 pKa = 4.4SKK62 pKa = 10.2AQTMQMGEE70 pKa = 4.06GGSFTNYY77 pKa = 9.19AYY79 pKa = 10.54QWFSMTKK86 pKa = 8.14YY87 pKa = 10.42PKK89 pKa = 9.29GTLVRR94 pKa = 11.84YY95 pKa = 9.88DD96 pKa = 4.13DD97 pKa = 4.27LVLEE101 pKa = 4.31IVEE104 pKa = 4.25VEE106 pKa = 4.58PYY108 pKa = 9.37WSQGGFAIYY117 pKa = 10.72YY118 pKa = 5.93MQNRR122 pKa = 11.84SDD124 pKa = 3.74TDD126 pKa = 3.6VQSS129 pKa = 3.33

Molecular weight: 14.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3BE82|A0A6M3BE82_9CAUD Uncharacterized protein OS=Lactobacillus phage JNU_P2 OX=2686382 PE=4 SV=1
MM1 pKa = 8.39VLMILFFSTAIYY13 pKa = 10.07LISLVYY19 pKa = 8.85ITRR22 pKa = 11.84EE23 pKa = 4.01TLRR26 pKa = 11.84KK27 pKa = 9.5SHH29 pKa = 5.96VAVSNRR35 pKa = 11.84SALVVLLILIGWNLKK50 pKa = 9.78ILFRR54 pKa = 11.84SRR56 pKa = 11.84FDD58 pKa = 2.9KK59 pKa = 10.99KK60 pKa = 10.65RR61 pKa = 11.84FYY63 pKa = 11.29KK64 pKa = 10.5LLKK67 pKa = 10.37SFLFEE72 pKa = 4.04YY73 pKa = 9.22PISVIVLGEE82 pKa = 4.24IIFSDD87 pKa = 3.39AKK89 pKa = 10.03KK90 pKa = 10.01HH91 pKa = 6.22SKK93 pKa = 10.29ISDD96 pKa = 3.19KK97 pKa = 11.27SVIHH101 pKa = 5.43VSISISGLSFNGLKK115 pKa = 9.94RR116 pKa = 11.84RR117 pKa = 11.84KK118 pKa = 9.27NKK120 pKa = 9.86QNLPIYY126 pKa = 9.37EE127 pKa = 4.13GLIAEE132 pKa = 4.79LL133 pKa = 4.12

Molecular weight: 15.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

14449

37

1929

203.5

22.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.333 ± 0.426

0.353 ± 0.063

6.776 ± 0.467

5.301 ± 0.376

3.523 ± 0.192

7.018 ± 0.691

2.139 ± 0.163

5.551 ± 0.271

8.118 ± 0.555

7.544 ± 0.238

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.748 ± 0.148

5.198 ± 0.222

3.433 ± 0.213

4.014 ± 0.238

4.353 ± 0.256

7.198 ± 0.331

6.686 ± 0.344

6.547 ± 0.264

1.308 ± 0.116

3.834 ± 0.257
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski