Thrips palmi (Melon thrips)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Paraneoptera; Thysanoptera; Terebrantia; Thripoidea; Thripidae; Thripinae; Thrips

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20971 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6P8ZIQ7|A0A6P8ZIQ7_THRPL Isoform of A0A6P8YJ99 Regulatory protein zeste OS=Thrips palmi OX=161013 GN=LOC117641037 PE=4 SV=1
MM1 pKa = 7.12EE2 pKa = 4.83TLDD5 pKa = 3.6TALSFSVFPPTPPPSTSSPSASSGSLWDD33 pKa = 3.57ADD35 pKa = 3.35LWGASSATWLDD46 pKa = 3.44EE47 pKa = 3.89GWALDD52 pKa = 3.7GLPDD56 pKa = 3.71VKK58 pKa = 10.74PDD60 pKa = 3.63VSEE63 pKa = 4.43LLQASLRR70 pKa = 11.84QDD72 pKa = 3.91LPVLNADD79 pKa = 3.48STTFLLDD86 pKa = 3.2GDD88 pKa = 4.51AAPAPAAWLPQWSYY102 pKa = 11.37CAYY105 pKa = 8.85CAAGGGVGAAGCGASVSQPLPPQGPSQLYY134 pKa = 8.64PDD136 pKa = 3.75QGADD140 pKa = 3.09QGADD144 pKa = 3.03QGGGYY149 pKa = 10.46LVDD152 pKa = 4.95PYY154 pKa = 10.96LGTLAGPMPAPFADD168 pKa = 3.32GLVFVDD174 pKa = 5.83SPALCPVLSTVPEE187 pKa = 4.27DD188 pKa = 3.48TCLEE192 pKa = 4.43DD193 pKa = 3.5AVHH196 pKa = 6.81WANPVPPAPSPLPAEE211 pKa = 4.31LSGHH215 pKa = 6.53RR216 pKa = 11.84SALPLPEE223 pKa = 4.89PPGKK227 pKa = 9.86SSKK230 pKa = 10.02RR231 pKa = 11.84APRR234 pKa = 11.84TSRR237 pKa = 11.84DD238 pKa = 3.26PFII241 pKa = 6.05

Molecular weight: 24.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6P9AEV0|A0A6P9AEV0_THRPL TOX high mobility group box family member 4-like isoform X1 OS=Thrips palmi OX=161013 GN=LOC117654355 PE=4 SV=1
MM1 pKa = 7.46AASLNAGFNTPLGNINFGLSQSSSNANSIGLGGGGGGGVGGGFGGSTAGANAGAGGASAGGQGAGGGFGGQGFGGQGFGGQGFGGQGFGGQGFGGQGFGGQGFGGQGFGGQGFGGQGFGGQGGFGGSRR129 pKa = 11.84PGGFGGPGGFGGSRR143 pKa = 11.84PGGFGGPGGFGGQGIGQSHH162 pKa = 6.05GQRR165 pKa = 11.84HH166 pKa = 4.88KK167 pKa = 10.84RR168 pKa = 11.84QFGFSGSSANAQSHH182 pKa = 5.88SGSGGFFGGGLGGLGASGANAGASSFGSGFGGLGGNLGGGLASSGANAGASSFGGGFGGLGGGLGASGANAGASSFGGGLGGLGGFGGLGGFGGFGGLGGLAGLGSGLGVSGANAGAGSFGAGGTHH308 pKa = 6.56HH309 pKa = 6.89GVGGGLSASQAASHH323 pKa = 6.32AASGAGGVGVGPTVGGGLSGSQASANAGSIGGGIGGGAGGAGGLSGSLANAQSGAGSIGGGVGLGGGVSGSQAAANAGSIGGGIGGGHH411 pKa = 6.59GFGGGLSGSQASANAGSIGGGIGGGAGGAGGLSGSLANAQSGAGSIGGGVGLGGGVTGSQAAANAGSLGGGIGGGHH487 pKa = 6.59GFGGGLSGSQASANAGSIGGGIGGGAGGAGGLSGSLANAQSGAGSIGGGVGLGGGVSGSQAAANAGSLSGGFGGGQGFGGGLSGSQASANAGSLSGGFGGGHH589 pKa = 6.39GFGGGLSGSQASANAGSLSGGFGGGHH615 pKa = 6.39GFGGGLSGSQASASAGSVAGGAGFGRR641 pKa = 4.21

Molecular weight: 53.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14300

6671

20971

13368287

37

16816

637.5

70.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.87 ± 0.022

1.929 ± 0.018

5.54 ± 0.014

6.47 ± 0.028

3.297 ± 0.013

6.371 ± 0.021

2.679 ± 0.009

3.965 ± 0.013

5.49 ± 0.022

9.234 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.293 ± 0.01

3.923 ± 0.012

6.177 ± 0.027

4.619 ± 0.022

5.896 ± 0.019

8.663 ± 0.028

5.453 ± 0.015

6.592 ± 0.015

1.087 ± 0.005

2.452 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski