Vibrio phage VHML

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Vhmlvirus; Vibrio virus VHML

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8H9K9|Q8H9K9_9CAUD ORF58 OS=Vibrio phage VHML OX=207597 GN=orf58 PE=4 SV=1
MM1 pKa = 7.68SDD3 pKa = 2.65WDD5 pKa = 3.67FTVAGFLIGEE15 pKa = 4.41YY16 pKa = 10.02GPVMSGEE23 pKa = 4.15LPYY26 pKa = 10.6HH27 pKa = 6.57EE28 pKa = 5.91LMQQEE33 pKa = 4.24AEE35 pKa = 4.2EE36 pKa = 4.21EE37 pKa = 4.44KK38 pKa = 10.36EE39 pKa = 4.51TVQLQCGLSGG49 pKa = 3.41

Molecular weight: 5.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8H9Q0|Q8H9Q0_9CAUD ORF15 OS=Vibrio phage VHML OX=207597 GN=orf15 PE=4 SV=1
MM1 pKa = 7.86DD2 pKa = 5.89KK3 pKa = 10.37IARR6 pKa = 11.84LPAISASANRR16 pKa = 11.84LKK18 pKa = 10.87AIWSKK23 pKa = 10.55VIKK26 pKa = 10.7VSIAQTAA33 pKa = 2.94

Molecular weight: 3.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

12043

33

1023

211.3

23.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.632 ± 0.674

1.187 ± 0.148

5.937 ± 0.32

7.1 ± 0.289

3.346 ± 0.199

7.183 ± 0.252

2.043 ± 0.209

4.874 ± 0.18

5.771 ± 0.286

9.068 ± 0.287

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.491 ± 0.173

4.011 ± 0.205

3.82 ± 0.245

4.75 ± 0.305

5.671 ± 0.294

5.904 ± 0.211

5.439 ± 0.168

7.058 ± 0.234

1.544 ± 0.163

3.114 ± 0.218
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski