Mycolicibacterium vanbaalenii (strain DSM 7251 / JCM 13017 / BCRC 16820 / KCTC 9966 / NRRL B-24157 / PYR-1) (Mycobacterium vanbaalenii)
Average proteome isoelectric point is 5.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5902 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|A1TBY9|RS20_MYCVP 30S ribosomal protein S20 OS=Mycolicibacterium vanbaalenii (strain DSM 7251 / JCM 13017 / BCRC 16820 / KCTC 9966 / NRRL B-24157 / PYR-1) OX=350058 GN=rpsT PE=3 SV=1
MM1 pKa = 7.94 ADD3 pKa = 3.28 NGLVNNVGAVRR14 pKa = 11.84 CAYY17 pKa = 10.32 AAGAGLAASVLLCACVSTVEE37 pKa = 4.42 GAAVRR42 pKa = 11.84 HH43 pKa = 6.18 PGAATLDD50 pKa = 3.7 VPPLDD55 pKa = 3.69 EE56 pKa = 4.75 AALDD60 pKa = 3.89 RR61 pKa = 11.84 VLLSVDD67 pKa = 3.6 EE68 pKa = 4.95 LNSIMGSIRR77 pKa = 11.84 MKK79 pKa = 10.56 VSSEE83 pKa = 3.61 LDD85 pKa = 3.08 EE86 pKa = 4.6 MTDD89 pKa = 3.19 HH90 pKa = 6.36 TAEE93 pKa = 4.12 VSNPDD98 pKa = 3.44 CLGTVYY104 pKa = 10.62 AAEE107 pKa = 4.23 EE108 pKa = 3.75 PAYY111 pKa = 10.44 AGTGWTAVRR120 pKa = 11.84 DD121 pKa = 3.92 QVARR125 pKa = 11.84 EE126 pKa = 3.73 PDD128 pKa = 3.39 GDD130 pKa = 3.64 NDD132 pKa = 3.26 HH133 pKa = 6.83 WVEE136 pKa = 4.22 QTAVIYY142 pKa = 9.02 PAARR146 pKa = 11.84 NAQDD150 pKa = 3.28 FFDD153 pKa = 4.45 RR154 pKa = 11.84 SSVTWQDD161 pKa = 3.31 CANATITTGDD171 pKa = 3.54 GDD173 pKa = 4.53 YY174 pKa = 11.05 LWEE177 pKa = 4.86 LDD179 pKa = 3.62 AAQVDD184 pKa = 4.52 DD185 pKa = 3.98 TVLTQRR191 pKa = 11.84 VTQDD195 pKa = 3.33 GADD198 pKa = 3.23 GWACQHH204 pKa = 6.37 ALSVVSNLTVEE215 pKa = 4.73 AWACGYY221 pKa = 10.51 SISDD225 pKa = 3.54 EE226 pKa = 4.02 AAEE229 pKa = 4.21 IAVAMIEE236 pKa = 4.13 NAATKK241 pKa = 10.35
Molecular weight: 25.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.717
IPC2_protein 3.884
IPC_protein 3.884
Toseland 3.668
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.795
Rodwell 3.706
Grimsley 3.579
Solomon 3.859
Lehninger 3.821
Nozaki 3.986
DTASelect 4.215
Thurlkill 3.719
EMBOSS 3.808
Sillero 3.999
Patrickios 1.189
IPC_peptide 3.859
IPC2_peptide 3.973
IPC2.peptide.svr19 3.886
Protein with the highest isoelectric point:
>tr|A1T140|A1T140_MYCVP Uncharacterized protein OS=Mycolicibacterium vanbaalenii (strain DSM 7251 / JCM 13017 / BCRC 16820 / KCTC 9966 / NRRL B-24157 / PYR-1) OX=350058 GN=Mvan_0039 PE=4 SV=1
MM1 pKa = 7.69 AKK3 pKa = 10.06 GKK5 pKa = 8.69 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 AKK17 pKa = 9.45 VHH19 pKa = 5.31 GFRR22 pKa = 11.84 LRR24 pKa = 11.84 MRR26 pKa = 11.84 TRR28 pKa = 11.84 AGRR31 pKa = 11.84 AIVTARR37 pKa = 11.84 RR38 pKa = 11.84 AKK40 pKa = 10.16 GRR42 pKa = 11.84 RR43 pKa = 11.84 SLTAA47 pKa = 3.9
Molecular weight: 5.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.705
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.427
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.238
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5902
0
5902
1947808
32
3696
330.0
35.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.069 ± 0.04
0.826 ± 0.008
6.447 ± 0.027
5.329 ± 0.027
3.052 ± 0.02
8.933 ± 0.037
2.184 ± 0.017
4.139 ± 0.02
2.081 ± 0.02
9.718 ± 0.031
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.057 ± 0.015
2.156 ± 0.016
5.853 ± 0.028
2.904 ± 0.016
7.296 ± 0.037
5.374 ± 0.022
6.121 ± 0.024
8.857 ± 0.03
1.5 ± 0.014
2.103 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here