Candidatus Nitrosotenuis uzonensis

Taxonomy: cellular organisms; Archaea; TACK group; Thaumarchaeota; Thaumarchaeota incertae sedis; Candidatus Nitrosotenuis

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1946 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V6AQC1|V6AQC1_9ARCH Rhomboid family protein OS=Candidatus Nitrosotenuis uzonensis OX=1407055 GN=NITUZ_140001 PE=4 SV=1
MM1 pKa = 7.92DD2 pKa = 3.95MLGNSMTQTQIVTVEE17 pKa = 3.91ACGKK21 pKa = 9.82PEE23 pKa = 3.66SAYY26 pKa = 11.11NKK28 pKa = 10.09IIGSEE33 pKa = 3.94DD34 pKa = 4.02DD35 pKa = 4.59DD36 pKa = 4.35VLFGTTLADD45 pKa = 4.88LIIGLGGDD53 pKa = 4.02DD54 pKa = 5.03IIYY57 pKa = 10.4GDD59 pKa = 4.67KK60 pKa = 11.52GNDD63 pKa = 3.72CILAGDD69 pKa = 4.48GEE71 pKa = 4.65DD72 pKa = 3.59VVYY75 pKa = 10.86GDD77 pKa = 4.08EE78 pKa = 4.56GNDD81 pKa = 3.54YY82 pKa = 11.26VHH84 pKa = 7.03GGDD87 pKa = 3.81GADD90 pKa = 3.43IIKK93 pKa = 10.11GQSGDD98 pKa = 3.56DD99 pKa = 3.62MLIGGLGNDD108 pKa = 4.61IIDD111 pKa = 4.57GGDD114 pKa = 3.38DD115 pKa = 3.57YY116 pKa = 11.73DD117 pKa = 4.04RR118 pKa = 11.84CIMTEE123 pKa = 3.63NDD125 pKa = 2.51NDD127 pKa = 3.66IVVKK131 pKa = 10.91CEE133 pKa = 3.76SS134 pKa = 3.26

Molecular weight: 14.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V6AU06|V6AU06_9ARCH Uncharacterized protein OS=Candidatus Nitrosotenuis uzonensis OX=1407055 GN=NITUZ_40094 PE=4 SV=1
MM1 pKa = 7.24ATHH4 pKa = 7.07GSITKK9 pKa = 9.64AGKK12 pKa = 10.39VKK14 pKa = 10.44GQTPKK19 pKa = 9.68VEE21 pKa = 3.71GRR23 pKa = 11.84KK24 pKa = 9.12RR25 pKa = 11.84VGTISILRR33 pKa = 11.84NKK35 pKa = 10.43NNFRR39 pKa = 11.84KK40 pKa = 9.96RR41 pKa = 11.84FALHH45 pKa = 5.87RR46 pKa = 11.84TPGQNKK52 pKa = 7.94PGQRR56 pKa = 11.84KK57 pKa = 8.67RR58 pKa = 11.84RR59 pKa = 3.71

Molecular weight: 6.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1946

0

1946

494545

24

3120

254.1

28.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.121 ± 0.055

0.985 ± 0.022

5.476 ± 0.04

6.335 ± 0.055

4.147 ± 0.04

6.579 ± 0.061

1.922 ± 0.025

8.451 ± 0.05

7.815 ± 0.067

8.735 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.746 ± 0.027

4.242 ± 0.035

3.832 ± 0.044

3.416 ± 0.034

4.451 ± 0.047

7.01 ± 0.055

5.665 ± 0.062

6.951 ± 0.046

0.885 ± 0.021

3.234 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski