Aureimonas sp. Leaf460

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Aurantimonadaceae; Aureimonas; unclassified Aureimonas

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4794 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q6CS85|A0A0Q6CS85_9RHIZ GntR family transcriptional regulator OS=Aureimonas sp. Leaf460 OX=1736384 GN=ASG54_25155 PE=3 SV=1
MM1 pKa = 7.25VATINLTTTNRR12 pKa = 11.84DD13 pKa = 2.95LVGYY17 pKa = 7.08FTNWTNGFGYY27 pKa = 10.31NGNGEE32 pKa = 4.46FSPLPFSGNQWAAGNVAGTTNTGVIMNGNLAYY64 pKa = 10.37VPPGGFTGTVNNLQFGTDD82 pKa = 3.87LNGSAATGFSLATAGLTVQIGDD104 pKa = 3.92APNATFNGAVYY115 pKa = 10.22SLSHH119 pKa = 6.55GGSFTGVTSASGTAQPGFYY138 pKa = 9.99DD139 pKa = 3.4YY140 pKa = 10.64FGEE143 pKa = 4.44VGTVQNGTNRR153 pKa = 11.84EE154 pKa = 3.99DD155 pKa = 3.26KK156 pKa = 10.9LYY158 pKa = 11.15SFDD161 pKa = 5.98GNDD164 pKa = 3.61TLNGGAGRR172 pKa = 11.84DD173 pKa = 3.43TFVFDD178 pKa = 4.94RR179 pKa = 11.84DD180 pKa = 3.06IDD182 pKa = 4.08GALSTTIGHH191 pKa = 6.22DD192 pKa = 3.34TVTGFVAGATGDD204 pKa = 4.26FINLQLQSTAFDD216 pKa = 3.7TFAEE220 pKa = 4.94VYY222 pKa = 10.24AAATQVGANTVINFGTLGDD241 pKa = 3.63ITLSNVTKK249 pKa = 9.79TALTADD255 pKa = 3.55NFVFAA260 pKa = 5.52

Molecular weight: 26.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q6EL19|A0A0Q6EL19_9RHIZ ABC transporter substrate-binding protein OS=Aureimonas sp. Leaf460 OX=1736384 GN=ASG54_10140 PE=3 SV=1
MM1 pKa = 7.85PIKK4 pKa = 10.47PEE6 pKa = 3.8LRR8 pKa = 11.84GFYY11 pKa = 10.21PIDD14 pKa = 3.23WRR16 pKa = 11.84EE17 pKa = 3.77LSHH20 pKa = 6.38SVRR23 pKa = 11.84FRR25 pKa = 11.84RR26 pKa = 11.84AKK28 pKa = 9.87GACEE32 pKa = 3.69RR33 pKa = 11.84CGRR36 pKa = 11.84PHH38 pKa = 6.64GRR40 pKa = 11.84IVCVLADD47 pKa = 3.77GRR49 pKa = 11.84WFDD52 pKa = 3.94EE53 pKa = 5.91DD54 pKa = 3.56RR55 pKa = 11.84SQWRR59 pKa = 11.84DD60 pKa = 2.95GRR62 pKa = 11.84GKK64 pKa = 10.28RR65 pKa = 11.84VRR67 pKa = 11.84TNIGAPAEE75 pKa = 4.03LPEE78 pKa = 4.62GFGHH82 pKa = 7.62RR83 pKa = 11.84STKK86 pKa = 10.31VVIACAHH93 pKa = 6.34LNHH96 pKa = 7.14DD97 pKa = 3.94VADD100 pKa = 3.91NAGGNLAALCQRR112 pKa = 11.84CHH114 pKa = 6.58MIHH117 pKa = 7.29DD118 pKa = 4.57RR119 pKa = 11.84PHH121 pKa = 5.78HH122 pKa = 5.32LARR125 pKa = 11.84RR126 pKa = 11.84RR127 pKa = 11.84ITYY130 pKa = 10.34LMRR133 pKa = 11.84RR134 pKa = 11.84AIGDD138 pKa = 4.22LFAGPYY144 pKa = 9.03RR145 pKa = 4.38

Molecular weight: 16.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4794

0

4794

1483502

30

2994

309.4

33.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.32 ± 0.058

0.716 ± 0.011

5.71 ± 0.028

6.067 ± 0.035

3.734 ± 0.024

8.915 ± 0.033

1.87 ± 0.018

5.016 ± 0.028

2.967 ± 0.028

10.244 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.31 ± 0.016

2.173 ± 0.019

5.187 ± 0.031

2.669 ± 0.019

7.514 ± 0.043

5.554 ± 0.023

5.35 ± 0.027

7.498 ± 0.031

1.188 ± 0.014

1.997 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski