Litchfieldia salsus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Litchfieldia

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4559 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H0TDH0|A0A1H0TDH0_9BACI Molybdenum cofactor cytidylyltransferase OS=Litchfieldia salsus OX=930152 GN=SAMN05216565_103415 PE=4 SV=1
MM1 pKa = 7.5SLEE4 pKa = 3.82WFDD7 pKa = 3.9RR8 pKa = 11.84VSGEE12 pKa = 4.11LQDD15 pKa = 4.16HH16 pKa = 6.79LEE18 pKa = 4.52SICDD22 pKa = 3.34KK23 pKa = 10.24YY24 pKa = 11.58DD25 pKa = 3.46QVGHH29 pKa = 5.36MSIDD33 pKa = 3.19RR34 pKa = 11.84GAKK37 pKa = 8.72HH38 pKa = 5.88PRR40 pKa = 11.84IEE42 pKa = 4.38FFVEE46 pKa = 4.15TTDD49 pKa = 4.6DD50 pKa = 3.75GEE52 pKa = 4.29RR53 pKa = 11.84DD54 pKa = 3.89YY55 pKa = 11.47FCSLFFDD62 pKa = 3.7PHH64 pKa = 7.26NEE66 pKa = 3.61EE67 pKa = 5.29FYY69 pKa = 11.35VEE71 pKa = 5.54SYY73 pKa = 11.46DD74 pKa = 5.76LDD76 pKa = 3.8LQQEE80 pKa = 4.29SRR82 pKa = 11.84AILLDD87 pKa = 3.22IEE89 pKa = 5.87DD90 pKa = 3.99IVDD93 pKa = 3.78CVHH96 pKa = 6.77EE97 pKa = 4.54SFHH100 pKa = 8.1EE101 pKa = 3.96YY102 pKa = 10.47MNDD105 pKa = 3.64DD106 pKa = 3.96EE107 pKa = 6.78DD108 pKa = 5.21DD109 pKa = 3.89EE110 pKa = 4.75EE111 pKa = 4.68YY112 pKa = 10.67EE113 pKa = 4.41YY114 pKa = 11.52YY115 pKa = 10.99LEE117 pKa = 4.21TTEE120 pKa = 6.29DD121 pKa = 4.53DD122 pKa = 3.95DD123 pKa = 6.35DD124 pKa = 6.57LYY126 pKa = 11.71LADD129 pKa = 5.89DD130 pKa = 4.32DD131 pKa = 5.21SEE133 pKa = 5.19NEE135 pKa = 4.0TEE137 pKa = 4.59VYY139 pKa = 10.38QEE141 pKa = 5.07IDD143 pKa = 3.87VEE145 pKa = 4.39WTTPEE150 pKa = 3.62VTAYY154 pKa = 10.01SHH156 pKa = 6.32EE157 pKa = 4.72DD158 pKa = 3.59EE159 pKa = 5.67VEE161 pKa = 3.79VTYY164 pKa = 10.93QFGIVQEE171 pKa = 4.4TGDD174 pKa = 3.51GVLRR178 pKa = 11.84RR179 pKa = 11.84VNRR182 pKa = 11.84VVTTDD187 pKa = 2.94EE188 pKa = 4.85DD189 pKa = 4.52LIEE192 pKa = 5.07DD193 pKa = 3.66EE194 pKa = 5.25SNFIFSKK201 pKa = 10.88EE202 pKa = 3.9EE203 pKa = 3.46ASTIIALIATHH214 pKa = 6.64MDD216 pKa = 3.34SLSDD220 pKa = 3.84FDD222 pKa = 6.25FDD224 pKa = 5.34MIDD227 pKa = 3.07GG228 pKa = 4.16

Molecular weight: 26.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H0UM01|A0A1H0UM01_9BACI NAD(P)-dependent dehydrogenase short-chain alcohol dehydrogenase family OS=Litchfieldia salsus OX=930152 GN=SAMN05216565_10517 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.21QPNNRR10 pKa = 11.84KK11 pKa = 9.13HH12 pKa = 6.54SKK14 pKa = 8.71VHH16 pKa = 5.75GFRR19 pKa = 11.84KK20 pKa = 10.02RR21 pKa = 11.84MSTKK25 pKa = 10.22NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4559

0

4559

1338924

28

2383

293.7

33.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.159 ± 0.037

0.7 ± 0.012

5.106 ± 0.033

7.59 ± 0.043

4.636 ± 0.035

6.759 ± 0.034

2.052 ± 0.019

8.367 ± 0.037

6.856 ± 0.034

9.815 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.659 ± 0.019

4.841 ± 0.028

3.479 ± 0.022

3.614 ± 0.026

3.765 ± 0.026

6.379 ± 0.029

5.559 ± 0.028

6.938 ± 0.033

1.04 ± 0.014

3.684 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski