Bacteroides acidifaciens

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3874 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3L8A5Z0|A0A3L8A5Z0_9BACE DUF5689 domain-containing protein OS=Bacteroides acidifaciens OX=85831 GN=D7Y07_12840 PE=4 SV=1
MM1 pKa = 6.9EE2 pKa = 5.64AKK4 pKa = 10.07VLSEE8 pKa = 3.7AKK10 pKa = 10.47VYY12 pKa = 10.65VGTYY16 pKa = 9.99AKK18 pKa = 10.32YY19 pKa = 10.81NNGSLFGAWLDD30 pKa = 3.89LSDD33 pKa = 4.65YY34 pKa = 10.83SDD36 pKa = 3.73KK37 pKa = 11.66EE38 pKa = 4.09EE39 pKa = 4.45FYY41 pKa = 9.88EE42 pKa = 4.99ACRR45 pKa = 11.84EE46 pKa = 4.05LHH48 pKa = 6.63KK49 pKa = 11.13DD50 pKa = 3.63EE51 pKa = 5.18EE52 pKa = 4.44DD53 pKa = 3.48AEE55 pKa = 4.74YY56 pKa = 10.09MFQDD60 pKa = 3.69WEE62 pKa = 4.32NVPEE66 pKa = 4.11GLIGEE71 pKa = 4.39SWISEE76 pKa = 4.08NFFSLRR82 pKa = 11.84DD83 pKa = 3.67AVEE86 pKa = 4.73DD87 pKa = 4.54LDD89 pKa = 4.64DD90 pKa = 4.21TEE92 pKa = 4.6QEE94 pKa = 5.44AFFVWCNYY102 pKa = 9.34KK103 pKa = 10.33SHH105 pKa = 7.42DD106 pKa = 4.14LSEE109 pKa = 4.49EE110 pKa = 4.04DD111 pKa = 3.38VDD113 pKa = 5.22DD114 pKa = 6.47LIRR117 pKa = 11.84DD118 pKa = 3.88FQDD121 pKa = 3.43EE122 pKa = 4.51YY123 pKa = 11.05QGQYY127 pKa = 11.09DD128 pKa = 3.94DD129 pKa = 5.56EE130 pKa = 5.07EE131 pKa = 5.7DD132 pKa = 3.22FAYY135 pKa = 10.08EE136 pKa = 4.79IIEE139 pKa = 4.02EE140 pKa = 4.7CYY142 pKa = 10.69NLPEE146 pKa = 3.93FAKK149 pKa = 10.16TYY151 pKa = 10.62FDD153 pKa = 3.75YY154 pKa = 11.44EE155 pKa = 4.0KK156 pKa = 10.43FARR159 pKa = 11.84DD160 pKa = 5.04LFMCDD165 pKa = 2.54YY166 pKa = 9.94WFDD169 pKa = 3.78DD170 pKa = 3.9GFVFRR175 pKa = 11.84AAA177 pKa = 3.87

Molecular weight: 21.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3L8A799|A0A3L8A799_9BACE DUF4374 domain-containing protein OS=Bacteroides acidifaciens OX=85831 GN=D7Y07_11450 PE=4 SV=1
MM1 pKa = 7.26AQALSGFQWNRR12 pKa = 11.84SSRR15 pKa = 11.84WIHH18 pKa = 5.9TIGLTDD24 pKa = 3.81IPNEE28 pKa = 3.89LLYY31 pKa = 11.16SQTNPTGGGFLCPQRR46 pKa = 11.84HH47 pKa = 5.18SKK49 pKa = 10.83VYY51 pKa = 9.89FQLPEE56 pKa = 4.08CFRR59 pKa = 11.84VTEE62 pKa = 4.18NPSTAVGRR70 pKa = 11.84IILRR74 pKa = 11.84SRR76 pKa = 11.84IISRR80 pKa = 11.84FLNQRR85 pKa = 11.84LNNGQAIKK93 pKa = 10.66LKK95 pKa = 10.3EE96 pKa = 3.8

Molecular weight: 10.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3874

0

3874

1394016

26

5085

359.8

40.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.991 ± 0.041

1.273 ± 0.017

5.58 ± 0.029

6.76 ± 0.04

4.522 ± 0.027

6.756 ± 0.035

1.821 ± 0.014

6.87 ± 0.04

6.874 ± 0.031

8.945 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.737 ± 0.019

5.2 ± 0.033

3.662 ± 0.02

3.495 ± 0.024

4.648 ± 0.029

6.089 ± 0.026

5.61 ± 0.027

6.375 ± 0.029

1.28 ± 0.015

4.513 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski