Rhodovulum robiginosum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Rhodovulum

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3552 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A428K0N7|A0A428K0N7_9RHOB LysE family translocator OS=Rhodovulum robiginosum OX=68292 GN=EJA01_00650 PE=4 SV=1
MM1 pKa = 7.41KK2 pKa = 9.29HH3 pKa = 4.39TTLVFAAAVAVMPFAGNAGEE23 pKa = 4.5PLAALDD29 pKa = 3.64VAEE32 pKa = 5.33DD33 pKa = 3.25ISRR36 pKa = 11.84FVFSAAPVFDD46 pKa = 5.65DD47 pKa = 5.32GMPAYY52 pKa = 9.95GNAFITQGYY61 pKa = 8.8LYY63 pKa = 10.04PAGTLDD69 pKa = 3.76GGVEE73 pKa = 4.17GTLPNGDD80 pKa = 3.87PAFPDD85 pKa = 3.56LVIGEE90 pKa = 4.45WTCDD94 pKa = 3.08GYY96 pKa = 11.43FIGDD100 pKa = 3.96GMRR103 pKa = 11.84TEE105 pKa = 4.29TGALVMTRR113 pKa = 11.84QVYY116 pKa = 9.5RR117 pKa = 11.84FNDD120 pKa = 3.34GDD122 pKa = 3.83VLISHH127 pKa = 6.84GAEE130 pKa = 3.68LVDD133 pKa = 4.38AGVTVTRR140 pKa = 11.84AVTGGTGDD148 pKa = 3.78YY149 pKa = 10.85ADD151 pKa = 4.88APAEE155 pKa = 3.98IDD157 pKa = 3.53QVLLGMSDD165 pKa = 3.67GYY167 pKa = 10.6GVRR170 pKa = 11.84LQIEE174 pKa = 4.48LGGGSADD181 pKa = 3.26AYY183 pKa = 10.52GDD185 pKa = 3.57EE186 pKa = 5.51DD187 pKa = 3.57HH188 pKa = 7.16TEE190 pKa = 4.03WEE192 pKa = 4.29SGFPVGPIPTSGSHH206 pKa = 5.42EE207 pKa = 4.2AGG209 pKa = 3.32

Molecular weight: 21.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3R9MHS6|A0A3R9MHS6_9RHOB Uncharacterized protein OS=Rhodovulum robiginosum OX=68292 GN=EJA01_00430 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3552

0

3552

1110346

28

2768

312.6

33.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.761 ± 0.068

0.875 ± 0.014

5.986 ± 0.038

5.994 ± 0.037

3.627 ± 0.025

9.213 ± 0.041

2.009 ± 0.02

4.639 ± 0.029

2.649 ± 0.034

10.259 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.623 ± 0.021

2.178 ± 0.022

5.443 ± 0.035

2.761 ± 0.021

7.359 ± 0.043

4.544 ± 0.029

5.16 ± 0.028

7.333 ± 0.033

1.404 ± 0.018

2.184 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski