Uncultured phage WW-nAnB strain 3

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; environmental samples

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W5S7M1|W5S7M1_9VIRU Protein D OS=Uncultured phage WW-nAnB strain 3 OX=1449897 PE=4 SV=1
MM1 pKa = 7.37MEE3 pKa = 4.65LPAIIKK9 pKa = 8.27TWVDD13 pKa = 3.69FDD15 pKa = 3.84GVTVYY20 pKa = 9.5TVQYY24 pKa = 10.39KK25 pKa = 10.69DD26 pKa = 4.21GSTCDD31 pKa = 3.44MTVQQYY37 pKa = 11.42DD38 pKa = 3.76YY39 pKa = 10.99LKK41 pKa = 11.05ASAQAVADD49 pKa = 3.83MDD51 pKa = 4.42SKK53 pKa = 11.62VSVDD57 pKa = 3.64SQPVEE62 pKa = 4.19TPVPAEE68 pKa = 3.56PAQNITEE75 pKa = 4.3SPDD78 pKa = 3.18LRR80 pKa = 11.84NGYY83 pKa = 9.35VPEE86 pKa = 4.03EE87 pKa = 4.13VEE89 pKa = 4.84LPFEE93 pKa = 4.74GSLTAYY99 pKa = 10.38DD100 pKa = 4.17DD101 pKa = 3.94RR102 pKa = 11.84AANTPALYY110 pKa = 10.8ANLPAVSNSFTAIMDD125 pKa = 3.72WFGDD129 pKa = 3.69TFFVEE134 pKa = 4.54RR135 pKa = 11.84TEE137 pKa = 4.44TVHH140 pKa = 6.82KK141 pKa = 10.29SGYY144 pKa = 6.93TSEE147 pKa = 4.33RR148 pKa = 11.84YY149 pKa = 9.6SYY151 pKa = 11.09NSSSQLIQLPYY162 pKa = 10.79EE163 pKa = 4.43EE164 pKa = 6.1DD165 pKa = 3.46STTTSQVLNPQACVSALLVVLVFITTVTWIKK196 pKa = 9.74NAIWGRR202 pKa = 11.84MSS204 pKa = 3.06

Molecular weight: 22.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W5S8E0|W5S8E0_9VIRU Protein G OS=Uncultured phage WW-nAnB strain 3 OX=1449897 PE=4 SV=1
MM1 pKa = 7.91RR2 pKa = 11.84SLSLALCMTSTLTATDD18 pKa = 3.14IFSEE22 pKa = 4.15LRR24 pKa = 11.84KK25 pKa = 10.66SNFLCSNPNFISYY38 pKa = 9.44GRR40 pKa = 11.84AVRR43 pKa = 11.84RR44 pKa = 11.84SHH46 pKa = 5.84MAGWCNGSTSRR57 pKa = 11.84SEE59 pKa = 4.16RR60 pKa = 11.84EE61 pKa = 4.16TVGSNPNPATKK72 pKa = 10.01TDD74 pKa = 3.4VTSVIRR80 pKa = 11.84CRR82 pKa = 11.84EE83 pKa = 3.82RR84 pKa = 11.84WWRR87 pKa = 11.84SEE89 pKa = 3.33KK90 pKa = 10.32KK91 pKa = 10.53AAVFLL96 pKa = 4.35

Molecular weight: 10.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

1366

37

378

170.8

19.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.564 ± 0.692

1.903 ± 0.512

6.296 ± 0.759

5.198 ± 0.871

6.442 ± 0.98

5.49 ± 0.625

1.391 ± 0.298

4.978 ± 0.772

4.173 ± 1.081

9.883 ± 1.003

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.196 ± 0.518

3.88 ± 0.369

4.685 ± 0.46

2.928 ± 0.459

4.758 ± 0.829

11.054 ± 1.787

5.051 ± 1.009

7.101 ± 0.824

2.123 ± 0.207

4.905 ± 0.564
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski