Torque teno midi virus 13
Average proteome isoelectric point is 7.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A7VM04|A7VM04_9VIRU Capsid protein OS=Torque teno midi virus 13 OX=2065054 PE=3 SV=1
MM1 pKa = 7.6 SKK3 pKa = 10.54 LCSTDD8 pKa = 3.79 FYY10 pKa = 11.33 KK11 pKa = 9.81 KK12 pKa = 9.17 TPFNPEE18 pKa = 3.5 TQTQIWMSQLADD30 pKa = 3.31 GHH32 pKa = 7.41 DD33 pKa = 3.98 NFCHH37 pKa = 6.3 CNSPFAHH44 pKa = 7.0 LLASIFPPGHH54 pKa = 7.17 KK55 pKa = 10.55 DD56 pKa = 3.08 RR57 pKa = 11.84 DD58 pKa = 3.61 LTINQILKK66 pKa = 9.78 RR67 pKa = 11.84 DD68 pKa = 3.9 YY69 pKa = 10.88 IEE71 pKa = 3.91 QCRR74 pKa = 11.84 STGEE78 pKa = 3.85 EE79 pKa = 4.56 GEE81 pKa = 4.21 NSGGEE86 pKa = 4.15 KK87 pKa = 9.54 PDD89 pKa = 3.15 QGGGFKK95 pKa = 10.74 GIEE98 pKa = 3.88 EE99 pKa = 4.16 KK100 pKa = 10.55 QQEE103 pKa = 4.51 EE104 pKa = 4.47 EE105 pKa = 4.71 EE106 pKa = 4.15 EE107 pKa = 4.03 DD108 pKa = 3.84 LPEE111 pKa = 6.05 KK112 pKa = 10.67 EE113 pKa = 4.99 LIDD116 pKa = 3.78 MLAAAAEE123 pKa = 4.36 DD124 pKa = 3.85 DD125 pKa = 3.91 TRR127 pKa = 5.45
Molecular weight: 14.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.543
IPC2_protein 4.52
IPC_protein 4.431
Toseland 4.279
ProMoST 4.533
Dawson 4.38
Bjellqvist 4.533
Wikipedia 4.24
Rodwell 4.279
Grimsley 4.19
Solomon 4.38
Lehninger 4.329
Nozaki 4.482
DTASelect 4.635
Thurlkill 4.279
EMBOSS 4.266
Sillero 4.546
Patrickios 3.808
IPC_peptide 4.38
IPC2_peptide 4.533
IPC2.peptide.svr19 4.483
Protein with the highest isoelectric point:
>tr|A7VM04|A7VM04_9VIRU Capsid protein OS=Torque teno midi virus 13 OX=2065054 PE=3 SV=1
MM1 pKa = 7.43 PFYY4 pKa = 10.16 WRR6 pKa = 11.84 RR7 pKa = 11.84 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.19 FWWGKK15 pKa = 9.63 ARR17 pKa = 11.84 PRR19 pKa = 11.84 WRR21 pKa = 11.84 FQRR24 pKa = 11.84 YY25 pKa = 7.03 RR26 pKa = 11.84 RR27 pKa = 11.84 KK28 pKa = 5.66 TTRR31 pKa = 11.84 RR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 PARR39 pKa = 11.84 KK40 pKa = 8.65 RR41 pKa = 11.84 AHH43 pKa = 6.84 RR44 pKa = 11.84 YY45 pKa = 7.37 AGRR48 pKa = 11.84 RR49 pKa = 11.84 RR50 pKa = 11.84 RR51 pKa = 11.84 RR52 pKa = 11.84 RR53 pKa = 11.84 YY54 pKa = 8.31 KK55 pKa = 10.32 VRR57 pKa = 11.84 RR58 pKa = 11.84 KK59 pKa = 8.94 RR60 pKa = 11.84 QKK62 pKa = 9.76 IPLFQWQPDD71 pKa = 3.98 KK72 pKa = 10.98 IVKK75 pKa = 10.19 CKK77 pKa = 10.4 IKK79 pKa = 10.73 GVTTLVLGAEE89 pKa = 5.12 GKK91 pKa = 9.98 QLVCYY96 pKa = 10.01 TNVKK100 pKa = 8.05 TANTPAKK107 pKa = 10.07 APGGGGFGCEE117 pKa = 3.92 QFSLDD122 pKa = 4.98 SLYY125 pKa = 11.21 ADD127 pKa = 3.12 YY128 pKa = 11.29 KK129 pKa = 10.08 LRR131 pKa = 11.84 KK132 pKa = 9.29 NVWTKK137 pKa = 11.52 SNILLDD143 pKa = 3.66 LCRR146 pKa = 11.84 YY147 pKa = 9.39 LFVKK151 pKa = 10.49 ISFYY155 pKa = 10.84 RR156 pKa = 11.84 HH157 pKa = 6.65 PEE159 pKa = 3.39 TDD161 pKa = 5.92 FIVTWHH167 pKa = 6.5 RR168 pKa = 11.84 QPPFDD173 pKa = 3.68 ISKK176 pKa = 10.11 EE177 pKa = 3.83 IYY179 pKa = 9.63 AACHH183 pKa = 5.22 PQNMLLAKK191 pKa = 10.0 HH192 pKa = 6.09 KK193 pKa = 10.14 MLILSKK199 pKa = 9.49 FTNPKK204 pKa = 10.22 GKK206 pKa = 9.96 LKK208 pKa = 9.37 KK209 pKa = 8.17 TRR211 pKa = 11.84 KK212 pKa = 9.27 VKK214 pKa = 10.21 PPKK217 pKa = 10.25 QMLNKK222 pKa = 9.47 WFFQEE227 pKa = 4.45 HH228 pKa = 6.44 FSHH231 pKa = 6.83 EE232 pKa = 4.38 PLLMIQATAANLNYY246 pKa = 10.23 VNLGCCNTNQMCTFFYY262 pKa = 10.34 INPGFYY268 pKa = 10.42 QIANWGATTTTGYY281 pKa = 9.98 IPYY284 pKa = 9.59 SGYY287 pKa = 8.23 PTATIYY293 pKa = 8.4 TWNYY297 pKa = 9.04 KK298 pKa = 7.54 QWQTGQGGQPFTKK311 pKa = 9.66 PQTYY315 pKa = 9.16 NQSVNYY321 pKa = 7.97 NTGFFSKK328 pKa = 10.59 QMLEE332 pKa = 4.05 AVQLTTDD339 pKa = 3.33 QTGQRR344 pKa = 11.84 PLGMNPVNICRR355 pKa = 11.84 YY356 pKa = 10.26 NPTLDD361 pKa = 3.51 SGKK364 pKa = 10.95 GNAIWLVSSHH374 pKa = 6.42 ANTYY378 pKa = 10.34 RR379 pKa = 11.84 EE380 pKa = 4.48 PITDD384 pKa = 3.12 KK385 pKa = 10.93 TLIFEE390 pKa = 4.51 GLPLYY395 pKa = 9.72 MLLYY399 pKa = 10.54 GYY401 pKa = 10.6 LSYY404 pKa = 10.62 VQKK407 pKa = 10.78 VKK409 pKa = 10.77 NVRR412 pKa = 11.84 DD413 pKa = 3.59 FLNNYY418 pKa = 5.78 TLCMRR423 pKa = 11.84 SPALFPYY430 pKa = 8.92 SQPGAEE436 pKa = 4.17 TQVIIPVNEE445 pKa = 3.76 NFIHH449 pKa = 6.92 GKK451 pKa = 9.35 AAYY454 pKa = 9.91 DD455 pKa = 3.52 EE456 pKa = 4.52 TLTEE460 pKa = 4.29 SVKK463 pKa = 10.94 GYY465 pKa = 6.22 WTPNVYY471 pKa = 9.66 QQIQTLNTMVEE482 pKa = 4.06 SSPYY486 pKa = 8.36 VPKK489 pKa = 10.93 YY490 pKa = 10.59 SQTKK494 pKa = 8.75 NSTWEE499 pKa = 3.46 LDD501 pKa = 3.34 MFYY504 pKa = 10.27 TFHH507 pKa = 7.24 FKK509 pKa = 10.02 WGWTRR514 pKa = 11.84 TNRR517 pKa = 11.84 PPITDD522 pKa = 3.65 PALQGTYY529 pKa = 9.19 EE530 pKa = 4.34 VPDD533 pKa = 3.85 SLQQAVQIRR542 pKa = 11.84 DD543 pKa = 3.44 PGKK546 pKa = 10.23 IKK548 pKa = 9.96 ATSILHH554 pKa = 5.94 PWDD557 pKa = 2.84 IRR559 pKa = 11.84 KK560 pKa = 10.05 GYY562 pKa = 7.78 FTTPALKK569 pKa = 10.5 RR570 pKa = 11.84 MSDD573 pKa = 3.38 NLSIDD578 pKa = 3.4 TTFQPDD584 pKa = 3.5 SEE586 pKa = 4.75 IIPTKK591 pKa = 10.04 RR592 pKa = 11.84 RR593 pKa = 11.84 KK594 pKa = 8.24 TGPEE598 pKa = 3.92 LSTPWEE604 pKa = 4.14 DD605 pKa = 3.18 QKK607 pKa = 11.46 EE608 pKa = 4.27 IQTCLQEE615 pKa = 4.41 LFKK618 pKa = 11.09 KK619 pKa = 9.56 STFQEE624 pKa = 4.12 EE625 pKa = 4.67 TEE627 pKa = 4.1 EE628 pKa = 4.41 DD629 pKa = 3.6 LHH631 pKa = 9.07 KK632 pKa = 10.84 LIQQQHH638 pKa = 4.34 QQQQEE643 pKa = 4.38 LKK645 pKa = 8.37 WNILRR650 pKa = 11.84 VISEE654 pKa = 4.35 IKK656 pKa = 10.23 QSQNLLKK663 pKa = 10.72 LQTGFPNN670 pKa = 3.45
Molecular weight: 78.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.212
IPC2_protein 9.458
IPC_protein 9.472
Toseland 10.014
ProMoST 9.736
Dawson 10.233
Bjellqvist 9.926
Wikipedia 10.409
Rodwell 10.657
Grimsley 10.321
Solomon 10.248
Lehninger 10.218
Nozaki 10.043
DTASelect 9.911
Thurlkill 10.087
EMBOSS 10.423
Sillero 10.16
Patrickios 9.999
IPC_peptide 10.248
IPC2_peptide 8.668
IPC2.peptide.svr19 8.417
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1330
127
670
332.5
38.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.887 ± 0.613
1.805 ± 0.163
4.586 ± 0.86
6.541 ± 1.495
4.586 ± 0.54
6.09 ± 0.543
2.481 ± 0.324
4.737 ± 0.345
8.722 ± 0.6
7.519 ± 0.232
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.955 ± 0.125
4.662 ± 0.564
6.767 ± 0.686
6.992 ± 0.522
5.94 ± 0.734
6.316 ± 2.027
7.444 ± 0.793
2.481 ± 0.923
1.729 ± 0.689
3.759 ± 0.947
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here