Magnaporthe oryzae chrysovirus 1
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E0D6V6|E0D6V6_9VIRU Uncharacterized protein OS=Magnaporthe oryzae chrysovirus 1 OX=764348 PE=4 SV=1
MM1 pKa = 7.64 GLTLDD6 pKa = 4.16 PAHH9 pKa = 7.39 RR10 pKa = 11.84 WRR12 pKa = 11.84 STDD15 pKa = 3.5 LAFAPVNEE23 pKa = 4.36 VSVQSAVRR31 pKa = 11.84 GRR33 pKa = 11.84 DD34 pKa = 3.35 DD35 pKa = 4.24 SEE37 pKa = 4.15 AAGTGFTGDD46 pKa = 3.49 KK47 pKa = 9.3 ATKK50 pKa = 9.54 AWYY53 pKa = 9.64 DD54 pKa = 3.25 GAGRR58 pKa = 11.84 VPIDD62 pKa = 4.63 DD63 pKa = 3.84 MCLAAGMRR71 pKa = 11.84 AGVLRR76 pKa = 11.84 LAVEE80 pKa = 4.44 VGSARR85 pKa = 11.84 PSSADD90 pKa = 3.09 EE91 pKa = 4.95 SVVQWRR97 pKa = 11.84 SVPYY101 pKa = 10.12 SYY103 pKa = 10.85 VGKK106 pKa = 9.06 PLTVSLSHH114 pKa = 7.21 AGRR117 pKa = 11.84 HH118 pKa = 4.62 FVARR122 pKa = 11.84 PALNEE127 pKa = 3.91 NVAMAMYY134 pKa = 8.0 EE135 pKa = 4.2 APTADD140 pKa = 2.93 KK141 pKa = 10.21 WVAATNFKK149 pKa = 10.66 LPRR152 pKa = 11.84 TVAAPGAAPQVPGLPNGGGGANLGLPNNFDD182 pKa = 3.12 AVRR185 pKa = 11.84 RR186 pKa = 11.84 VLVEE190 pKa = 3.67 CARR193 pKa = 11.84 GDD195 pKa = 3.55 DD196 pKa = 3.38 YY197 pKa = 11.65 GYY199 pKa = 11.24 RR200 pKa = 11.84 LFSMARR206 pKa = 11.84 VVLHH210 pKa = 6.77 AEE212 pKa = 3.91 TMRR215 pKa = 11.84 RR216 pKa = 11.84 SGISPRR222 pKa = 11.84 ATPSMDD228 pKa = 3.56 DD229 pKa = 3.18 QNMFSITTGDD239 pKa = 3.72 TPHH242 pKa = 6.5 LTEE245 pKa = 4.48 AQINNYY251 pKa = 9.7 AYY253 pKa = 10.17 AYY255 pKa = 10.04 NHH257 pKa = 6.41 TEE259 pKa = 3.34 QSPQYY264 pKa = 10.13 RR265 pKa = 11.84 AFLAMGLRR273 pKa = 11.84 GVGHH277 pKa = 5.73 YY278 pKa = 9.71 AIPGTIYY285 pKa = 10.8 SDD287 pKa = 2.78 GDD289 pKa = 3.98 YY290 pKa = 10.87 PVEE293 pKa = 4.22 CAVNHH298 pKa = 6.7 PIAFVRR304 pKa = 11.84 VGGPPPANVAPDD316 pKa = 3.88 PAHH319 pKa = 5.06 YY320 pKa = 8.69 TAVLSNPGLALSYY333 pKa = 9.15 YY334 pKa = 8.13 WAYY337 pKa = 10.18 AYY339 pKa = 11.39 SMGLGRR345 pKa = 11.84 VAGAILAQASIAPHH359 pKa = 5.54 IWGSAAVAPYY369 pKa = 10.38 KK370 pKa = 10.89 NCTPKK375 pKa = 10.63 LDD377 pKa = 3.55 AAAYY381 pKa = 10.23 LLLPDD386 pKa = 4.17 QEE388 pKa = 4.75 TAHH391 pKa = 5.55 VTADD395 pKa = 3.31 SARR398 pKa = 11.84 EE399 pKa = 3.96 LVANAAVLSEE409 pKa = 4.8 AYY411 pKa = 10.15 LAGIGATLLSARR423 pKa = 11.84 DD424 pKa = 3.69 SGHH427 pKa = 6.42 QDD429 pKa = 2.68 TALMMRR435 pKa = 11.84 AVTEE439 pKa = 4.1 KK440 pKa = 10.88 LSDD443 pKa = 3.43 PEE445 pKa = 4.17 TRR447 pKa = 11.84 RR448 pKa = 11.84 GAMLSITSRR457 pKa = 11.84 LCPGGVGMEE466 pKa = 4.07 WLSPFSYY473 pKa = 10.7 DD474 pKa = 4.02 VLDD477 pKa = 3.75 GTEE480 pKa = 3.63 RR481 pKa = 11.84 CIRR484 pKa = 11.84 AWRR487 pKa = 11.84 NHH489 pKa = 4.84 GFLLALYY496 pKa = 7.47 DD497 pKa = 4.03 TSPVAALAPLFSTGVPMNNSLLDD520 pKa = 3.64 KK521 pKa = 10.73 KK522 pKa = 11.04 SVVTGAEE529 pKa = 4.05 YY530 pKa = 9.47 PQLVACALAGRR541 pKa = 11.84 AEE543 pKa = 3.93 LAGRR547 pKa = 11.84 CEE549 pKa = 4.39 RR550 pKa = 11.84 PSPAYY555 pKa = 10.0 LAALAGHH562 pKa = 6.14 SARR565 pKa = 11.84 MRR567 pKa = 11.84 AWTVVVTVLGVVPPASDD584 pKa = 4.64 DD585 pKa = 3.57 EE586 pKa = 4.45 DD587 pKa = 3.96 HH588 pKa = 7.87 ADD590 pKa = 3.64 VQEE593 pKa = 4.28 QAASRR598 pKa = 11.84 QEE600 pKa = 4.07 SSSSVRR606 pKa = 11.84 PLSSQSDD613 pKa = 3.44 RR614 pKa = 11.84 SVTRR618 pKa = 11.84 GHH620 pKa = 6.0 GTAEE624 pKa = 4.08 PAQAGAGPSSPPPVAPLRR642 pKa = 11.84 GMRR645 pKa = 11.84 LGSRR649 pKa = 11.84 ARR651 pKa = 11.84 SHH653 pKa = 6.46 KK654 pKa = 10.44 GSLSAPQDD662 pKa = 4.46 PIPEE666 pKa = 4.29 TGEE669 pKa = 3.95 EE670 pKa = 4.38 PVRR673 pKa = 11.84 QPDD676 pKa = 3.64 GAGAKK681 pKa = 9.85 PKK683 pKa = 10.32 VQAILEE689 pKa = 4.31 APKK692 pKa = 9.98 PALEE696 pKa = 4.28 PPSWNSWASEE706 pKa = 4.21 VASVEE711 pKa = 3.82 ARR713 pKa = 11.84 LAGADD718 pKa = 3.34 EE719 pKa = 4.67 GPRR722 pKa = 11.84 GKK724 pKa = 9.61 IVEE727 pKa = 4.01 PEE729 pKa = 3.82 YY730 pKa = 10.93 RR731 pKa = 11.84 GIAPSRR737 pKa = 11.84 STTTVATSRR746 pKa = 11.84 SVVPSAASAASGTVVSMGRR765 pKa = 11.84 RR766 pKa = 11.84 AKK768 pKa = 9.97 GKK770 pKa = 8.31 EE771 pKa = 3.96 RR772 pKa = 11.84 EE773 pKa = 4.17 VEE775 pKa = 3.78 RR776 pKa = 11.84 ADD778 pKa = 3.58 QVEE781 pKa = 4.41 SSSGSSDD788 pKa = 3.28 QASGGSRR795 pKa = 11.84 GEE797 pKa = 4.04 EE798 pKa = 3.84 LL799 pKa = 4.08
Molecular weight: 84.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.994
IPC2_protein 5.944
IPC_protein 6.02
Toseland 6.415
ProMoST 6.389
Dawson 6.313
Bjellqvist 6.313
Wikipedia 6.326
Rodwell 6.3
Grimsley 6.561
Solomon 6.313
Lehninger 6.313
Nozaki 6.59
DTASelect 6.781
Thurlkill 6.795
EMBOSS 6.766
Sillero 6.693
Patrickios 4.215
IPC_peptide 6.326
IPC2_peptide 6.664
IPC2.peptide.svr19 6.608
Protein with the highest isoelectric point:
>tr|I7H3R3|I7H3R3_9VIRU Uncharacterized protein OS=Magnaporthe oryzae chrysovirus 1 OX=764348 PE=4 SV=1
MM1 pKa = 7.99 DD2 pKa = 5.73 AKK4 pKa = 10.81 QSGAGARR11 pKa = 11.84 VSGWWPDD18 pKa = 3.09 RR19 pKa = 11.84 QVIGRR24 pKa = 11.84 EE25 pKa = 3.91 ALRR28 pKa = 11.84 QRR30 pKa = 11.84 TSRR33 pKa = 11.84 TLGAMGGAVGVSDD46 pKa = 3.88 TPRR49 pKa = 11.84 SFFGGGEE56 pKa = 4.18 VPTEE60 pKa = 3.77 RR61 pKa = 11.84 LRR63 pKa = 11.84 RR64 pKa = 11.84 GLPSEE69 pKa = 4.09 VAGMFTAEE77 pKa = 4.45 QLSEE81 pKa = 4.21 MLDD84 pKa = 3.3 MSAEE88 pKa = 4.0 QRR90 pKa = 11.84 VKK92 pKa = 10.97 ALADD96 pKa = 2.89 AWEE99 pKa = 4.21 RR100 pKa = 11.84 LAAAEE105 pKa = 4.37 ASTGPTSSGPQTPARR120 pKa = 11.84 RR121 pKa = 11.84 APRR124 pKa = 11.84 SVSDD128 pKa = 3.64 YY129 pKa = 10.65 NTTQWVAAAANDD141 pKa = 5.24 GIRR144 pKa = 11.84 VTPTLTAAARR154 pKa = 11.84 AVAPALNRR162 pKa = 11.84 AVVPGGCQPHH172 pKa = 6.68 ASYY175 pKa = 11.28 GRR177 pKa = 11.84 ATVATGTGDD186 pKa = 3.26 IAPPSARR193 pKa = 11.84 QTAVVQAIGEE203 pKa = 4.47 SVPTEE208 pKa = 3.88 PVDD211 pKa = 4.28 AEE213 pKa = 4.5 VKK215 pKa = 10.53 EE216 pKa = 4.65 GTPSPAAPPRR226 pKa = 11.84 LLCGTATLLALPQVCCGSALDD247 pKa = 4.58 HH248 pKa = 6.55 GCAAAPCAVGQVSGAAAAVALASRR272 pKa = 11.84 PVVEE276 pKa = 4.87 PDD278 pKa = 3.23 ADD280 pKa = 3.54 ASSMGEE286 pKa = 3.52 RR287 pKa = 11.84 WKK289 pKa = 11.33 VMSTLAARR297 pKa = 11.84 LGPIAQCHH305 pKa = 5.74 FGSCRR310 pKa = 11.84 DD311 pKa = 3.41 VCACTHH317 pKa = 6.11 RR318 pKa = 11.84 HH319 pKa = 5.09 PLARR323 pKa = 11.84 ALMGIGVGTEE333 pKa = 3.86 EE334 pKa = 3.77 LRR336 pKa = 11.84 GARR339 pKa = 11.84 VHH341 pKa = 6.32 LTTPQEE347 pKa = 4.27 SRR349 pKa = 11.84 SVASVLLEE357 pKa = 3.87 KK358 pKa = 10.92 VGGGSRR364 pKa = 11.84 VEE366 pKa = 3.83 RR367 pKa = 11.84 LAVEE371 pKa = 4.36 FVKK374 pKa = 10.95 AVDD377 pKa = 4.28 GPVLTSLVVASRR389 pKa = 11.84 DD390 pKa = 3.48 GKK392 pKa = 10.93 ADD394 pKa = 3.54 NLLTLVAEE402 pKa = 4.86 AMAQGSLAAPRR413 pKa = 11.84 TMVTFGRR420 pKa = 11.84 GDD422 pKa = 3.26 GGGPAFDD429 pKa = 4.46 PNKK432 pKa = 10.74 LYY434 pKa = 10.46 DD435 pKa = 3.44 ACVYY439 pKa = 10.53 SGEE442 pKa = 4.34 RR443 pKa = 11.84 GHH445 pKa = 7.02 KK446 pKa = 10.37 LGVLSRR452 pKa = 11.84 AVWLEE457 pKa = 3.55 SDD459 pKa = 5.11 ADD461 pKa = 4.17 VHH463 pKa = 6.53 WMPHH467 pKa = 5.24 SNSLRR472 pKa = 11.84 VTLQCWNRR480 pKa = 11.84 RR481 pKa = 11.84 GDD483 pKa = 3.94 CKK485 pKa = 10.81 GVSAVIGPTTWRR497 pKa = 11.84 PHH499 pKa = 5.75 VGNSSWEE506 pKa = 4.08 TLGSDD511 pKa = 4.89 VYY513 pKa = 11.08 RR514 pKa = 11.84 DD515 pKa = 3.23 WFGLVVARR523 pKa = 11.84 RR524 pKa = 11.84 GGGDD528 pKa = 3.03 TTVRR532 pKa = 11.84 HH533 pKa = 5.13 QLSYY537 pKa = 10.86 NPGMEE542 pKa = 4.09 ADD544 pKa = 3.55 QLGGLLAHH552 pKa = 6.91 SGCGVYY558 pKa = 10.64 VSGADD563 pKa = 3.79 DD564 pKa = 4.17 AATVAAVHH572 pKa = 6.62 PSLQVTYY579 pKa = 7.87 THH581 pKa = 6.6 ITLGSPTASKK591 pKa = 10.57 LVQAATALDD600 pKa = 3.65 EE601 pKa = 4.32 FVRR604 pKa = 11.84 RR605 pKa = 11.84 GGAHH609 pKa = 6.43 RR610 pKa = 11.84 PP611 pKa = 3.54
Molecular weight: 63.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.997
IPC2_protein 7.19
IPC_protein 7.073
Toseland 6.825
ProMoST 7.717
Dawson 7.732
Bjellqvist 8.185
Wikipedia 7.644
Rodwell 7.746
Grimsley 6.912
Solomon 7.775
Lehninger 7.79
Nozaki 8.536
DTASelect 7.922
Thurlkill 7.966
EMBOSS 7.98
Sillero 8.302
Patrickios 4.507
IPC_peptide 7.775
IPC2_peptide 7.717
IPC2.peptide.svr19 7.313
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
4283
611
1127
856.6
91.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.565 ± 0.417
1.378 ± 0.154
5.463 ± 0.25
5.09 ± 0.435
1.915 ± 0.365
9.339 ± 0.721
2.241 ± 0.055
2.241 ± 0.277
2.405 ± 0.486
8.686 ± 0.605
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.358 ± 0.214
2.405 ± 0.172
5.907 ± 0.642
3.222 ± 0.172
7.798 ± 0.151
7.635 ± 0.488
5.347 ± 0.51
8.545 ± 0.283
1.471 ± 0.173
2.989 ± 0.371
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here