Maylandia zebra (zebra mbuna)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha; Neoteleostei<

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 37295 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3P9CSE2|A0A3P9CSE2_9CICH Nucleoporin 155 OS=Maylandia zebra OX=106582 PE=3 SV=1
MM1 pKa = 7.52LWFLGLTCVLCIGGSAVAGQRR22 pKa = 11.84DD23 pKa = 4.16GEE25 pKa = 4.35IISQIKK31 pKa = 6.94MTNLVLGEE39 pKa = 4.07IKK41 pKa = 10.42EE42 pKa = 4.1LLKK45 pKa = 10.61QQIKK49 pKa = 10.53EE50 pKa = 3.89IVFLKK55 pKa = 8.75NTVMEE60 pKa = 4.76CEE62 pKa = 4.0ACAMGGMQPQPTCVPNSCHH81 pKa = 6.45PGVSFVEE88 pKa = 4.5TPEE91 pKa = 4.08GVKK94 pKa = 10.48CGPCPHH100 pKa = 6.8GMEE103 pKa = 5.14GNGTHH108 pKa = 5.99CTDD111 pKa = 3.66VDD113 pKa = 3.7EE114 pKa = 4.91CTVKK118 pKa = 10.02PCHH121 pKa = 6.14MGVRR125 pKa = 11.84CINTSPGFRR134 pKa = 11.84CGSCPAGYY142 pKa = 8.41TGPQVQGVGLAYY154 pKa = 9.21ATANKK159 pKa = 9.17QVCRR163 pKa = 11.84DD164 pKa = 3.32INEE167 pKa = 4.25CDD169 pKa = 3.5GPNNGGCVANSVCLNTPGSFRR190 pKa = 11.84CGPCKK195 pKa = 9.95TGYY198 pKa = 8.97TGDD201 pKa = 3.59QQQGCKK207 pKa = 9.73PEE209 pKa = 4.04RR210 pKa = 11.84ACGNGQPNPCHH221 pKa = 6.71ASAEE225 pKa = 4.62CIVHH229 pKa = 6.66RR230 pKa = 11.84EE231 pKa = 4.01GTIEE235 pKa = 4.18CQCGVGWAGNGYY247 pKa = 9.89VCGPDD252 pKa = 3.17TDD254 pKa = 4.09IDD256 pKa = 3.94GFPDD260 pKa = 3.58EE261 pKa = 5.92KK262 pKa = 11.02LDD264 pKa = 5.4CPDD267 pKa = 3.83SNCNKK272 pKa = 10.03DD273 pKa = 3.32NCLTVPNSGQEE284 pKa = 4.12DD285 pKa = 4.09ADD287 pKa = 3.68SDD289 pKa = 4.74GIGDD293 pKa = 4.47ACDD296 pKa = 3.53EE297 pKa = 4.74DD298 pKa = 5.69ADD300 pKa = 4.77GDD302 pKa = 4.76GILNTQDD309 pKa = 3.01NCVLVPNVDD318 pKa = 3.12QRR320 pKa = 11.84NIDD323 pKa = 3.42EE324 pKa = 5.56DD325 pKa = 4.48DD326 pKa = 4.81FGDD329 pKa = 4.55ACDD332 pKa = 3.7NCPAVKK338 pKa = 10.8NNDD341 pKa = 3.5QKK343 pKa = 11.29DD344 pKa = 3.71TDD346 pKa = 3.32VDD348 pKa = 3.94KK349 pKa = 11.9YY350 pKa = 11.35GDD352 pKa = 3.78EE353 pKa = 4.36CDD355 pKa = 3.89EE356 pKa = 5.85DD357 pKa = 4.15IDD359 pKa = 5.21GDD361 pKa = 4.45GIPNHH366 pKa = 7.08LDD368 pKa = 2.97NCKK371 pKa = 9.84RR372 pKa = 11.84VPNADD377 pKa = 3.2QKK379 pKa = 11.71DD380 pKa = 3.63RR381 pKa = 11.84DD382 pKa = 3.66GDD384 pKa = 4.12KK385 pKa = 11.46VGDD388 pKa = 4.05ACDD391 pKa = 3.46SCPYY395 pKa = 10.53DD396 pKa = 3.99PNPDD400 pKa = 3.95QMDD403 pKa = 4.12ADD405 pKa = 4.07NDD407 pKa = 4.82LIGDD411 pKa = 3.97PCDD414 pKa = 3.62TNKK417 pKa = 10.93DD418 pKa = 3.49SDD420 pKa = 4.16GDD422 pKa = 3.81GHH424 pKa = 6.91QDD426 pKa = 4.2SRR428 pKa = 11.84DD429 pKa = 3.47NCPAVINSSQLDD441 pKa = 3.5TDD443 pKa = 3.78KK444 pKa = 11.37DD445 pKa = 4.07GKK447 pKa = 11.12GDD449 pKa = 3.71EE450 pKa = 5.3CDD452 pKa = 5.61DD453 pKa = 5.63DD454 pKa = 6.35DD455 pKa = 7.03DD456 pKa = 6.2NDD458 pKa = 5.44GIPDD462 pKa = 4.89LLPPGPDD469 pKa = 2.97NCRR472 pKa = 11.84LIPNPLQEE480 pKa = 5.54DD481 pKa = 3.65SDD483 pKa = 4.45GDD485 pKa = 4.06GVGNVCEE492 pKa = 4.73KK493 pKa = 11.11DD494 pKa = 3.31FDD496 pKa = 4.13NDD498 pKa = 4.31TIIDD502 pKa = 4.92PIDD505 pKa = 3.6VCPEE509 pKa = 3.74NAEE512 pKa = 4.04VTLTDD517 pKa = 3.59FRR519 pKa = 11.84EE520 pKa = 4.27YY521 pKa = 9.18QTVVLDD527 pKa = 4.15PEE529 pKa = 5.32GDD531 pKa = 3.65AQIDD535 pKa = 4.13PNWVVLNQGRR545 pKa = 11.84EE546 pKa = 3.9IVQTMNSDD554 pKa = 3.19PGLAVGYY561 pKa = 7.12TAFSGVDD568 pKa = 3.72FEE570 pKa = 5.24GTFHH574 pKa = 6.81VNTVTDD580 pKa = 3.57DD581 pKa = 4.08DD582 pKa = 4.34YY583 pKa = 12.0AGFIFAYY590 pKa = 9.51QDD592 pKa = 3.06SSSFYY597 pKa = 9.91VVMWKK602 pKa = 9.52QVEE605 pKa = 4.18QIYY608 pKa = 8.33WQANPFRR615 pKa = 11.84AVAEE619 pKa = 4.08PGIQLKK625 pKa = 10.06AVKK628 pKa = 10.35SNTGPGEE635 pKa = 4.06NLRR638 pKa = 11.84NALWHH643 pKa = 6.46TGDD646 pKa = 3.25TTDD649 pKa = 4.87QVKK652 pKa = 10.44LLWKK656 pKa = 10.05DD657 pKa = 3.18ARR659 pKa = 11.84NVGWKK664 pKa = 10.41DD665 pKa = 3.0KK666 pKa = 10.83TSYY669 pKa = 10.52RR670 pKa = 11.84WFLQHH675 pKa = 6.87RR676 pKa = 11.84PADD679 pKa = 3.73GYY681 pKa = 10.45IRR683 pKa = 11.84VRR685 pKa = 11.84FYY687 pKa = 11.29EE688 pKa = 4.41GTQMVADD695 pKa = 3.86TGVIIDD701 pKa = 3.35ATMRR705 pKa = 11.84GGRR708 pKa = 11.84LGVFCFSQEE717 pKa = 3.95NIIWANLRR725 pKa = 11.84YY726 pKa = 9.71RR727 pKa = 11.84CNDD730 pKa = 3.48TLPEE734 pKa = 4.48DD735 pKa = 3.93FDD737 pKa = 4.54AYY739 pKa = 10.45RR740 pKa = 11.84AQQVQLVAA748 pKa = 4.4

Molecular weight: 81.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3P9CG60|A0A3P9CG60_9CICH Ankyrin repeat and kinase domain containing 1 OS=Maylandia zebra OX=106582 PE=4 SV=1
MM1 pKa = 7.55CIVSGVLAIRR11 pKa = 11.84SLSAPSQPFTAQSAPSQPFTAQSAPSQPFTAQSAPSQPFTAQSAPSQPFTAQSAPSQPFTAQSAPSQPFTAQSAPSQPFTAPLAPSQPFTAPLAPSQPFTAPLAPSQPFTAPLAPSQPFTAPLAPSQPFTAPLAPSQPFTAPLAPSQPPSVQSPVSPLAQPLTPPQAPFSPLPSLQSSVRR191 pKa = 11.84SATQSPLAPSSTLPPVSLPPQPSLHH216 pKa = 7.0PATQAATQSVIQPILRR232 pKa = 11.84LARR235 pKa = 11.84PQPLRR240 pKa = 11.84PRR242 pKa = 11.84LARR245 pKa = 11.84PPQPLRR251 pKa = 11.84PRR253 pKa = 11.84LARR256 pKa = 11.84PPQPLRR262 pKa = 11.84PRR264 pKa = 11.84LARR267 pKa = 11.84PPQSLRR273 pKa = 11.84PRR275 pKa = 11.84LARR278 pKa = 11.84PPQSLRR284 pKa = 11.84PRR286 pKa = 11.84LARR289 pKa = 11.84PPQSLRR295 pKa = 11.84LLQPQPVLRR304 pKa = 11.84LVPPP308 pKa = 4.66

Molecular weight: 32.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

26988

10307

37295

20228919

17

6169

542.4

60.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.417 ± 0.01

2.329 ± 0.01

5.135 ± 0.009

6.74 ± 0.016

3.75 ± 0.009

6.202 ± 0.016

2.658 ± 0.006

4.574 ± 0.01

5.766 ± 0.015

9.552 ± 0.016

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.342 ± 0.005

3.951 ± 0.008

5.55 ± 0.017

4.667 ± 0.012

5.494 ± 0.012

8.739 ± 0.016

5.751 ± 0.01

6.38 ± 0.01

1.174 ± 0.005

2.826 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski