Fusobacterium phage Fnu1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Latrobevirus; Fusobacterium virus FNU1

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 181 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A481W667|A0A481W667_9CAUD Uncharacterized protein OS=Fusobacterium phage Fnu1 OX=2530024 PE=4 SV=1
MM1 pKa = 7.48NRR3 pKa = 11.84EE4 pKa = 3.78DD5 pKa = 3.97WVNLIYY11 pKa = 10.26EE12 pKa = 4.32IQDD15 pKa = 3.39SYY17 pKa = 11.65FDD19 pKa = 4.97SICLSNDD26 pKa = 2.58IQEE29 pKa = 4.56LCDD32 pKa = 3.73YY33 pKa = 9.82PIEE36 pKa = 4.17ITVDD40 pKa = 3.36EE41 pKa = 5.05EE42 pKa = 4.4GDD44 pKa = 3.63NVIISQDD51 pKa = 3.15SNYY54 pKa = 10.46YY55 pKa = 9.77IQFKK59 pKa = 10.89YY60 pKa = 10.87NITKK64 pKa = 10.61DD65 pKa = 4.01EE66 pKa = 4.14LVHH69 pKa = 8.74IYY71 pKa = 7.42FTRR74 pKa = 11.84LWRR77 pKa = 11.84RR78 pKa = 11.84DD79 pKa = 3.16

Molecular weight: 9.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A481W676|A0A481W676_9CAUD dUTP diphosphatase OS=Fusobacterium phage Fnu1 OX=2530024 PE=4 SV=1
MM1 pKa = 7.29GSSVGRR7 pKa = 11.84TLVLGTRR14 pKa = 11.84SRR16 pKa = 11.84EE17 pKa = 4.2FKK19 pKa = 10.84SLPTDD24 pKa = 3.15HH25 pKa = 6.9NMPYY29 pKa = 9.33YY30 pKa = 10.77LKK32 pKa = 10.66GRR34 pKa = 11.84GSISRR39 pKa = 11.84NKK41 pKa = 9.22RR42 pKa = 11.84CGFEE46 pKa = 4.75SYY48 pKa = 10.78FKK50 pKa = 11.42LEE52 pKa = 4.3GLNSLIGKK60 pKa = 8.89SLVNYY65 pKa = 9.71RR66 pKa = 11.84KK67 pKa = 10.3VSVQVV72 pKa = 3.22

Molecular weight: 8.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

181

0

181

38254

52

1974

211.3

24.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.158 ± 0.186

1.218 ± 0.097

6.164 ± 0.148

8.023 ± 0.217

4.316 ± 0.13

4.321 ± 0.16

1.179 ± 0.073

9.196 ± 0.23

10.72 ± 0.21

9.711 ± 0.198

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.397 ± 0.11

7.665 ± 0.203

1.893 ± 0.106

3.458 ± 0.174

3.563 ± 0.116

5.348 ± 0.199

5.5 ± 0.184

5.257 ± 0.137

1.001 ± 0.063

5.895 ± 0.188
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski