Xylaria flabelliformis
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11403 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A553HZZ0|A0A553HZZ0_9PEZI M20_dimer domain-containing protein OS=Xylaria flabelliformis OX=2512241 GN=FHL15_005492 PE=3 SV=1
MM1 pKa = 7.24 PLKK4 pKa = 10.81 YY5 pKa = 11.15 NMMDD9 pKa = 3.82 LCRR12 pKa = 11.84 SDD14 pKa = 3.28 NLRR17 pKa = 11.84 YY18 pKa = 9.87 VYY20 pKa = 10.31 YY21 pKa = 10.72 GQIWWDD27 pKa = 3.51 NDD29 pKa = 3.23 SDD31 pKa = 4.52 PDD33 pKa = 4.06 NLYY36 pKa = 11.11 DD37 pKa = 6.55 LDD39 pKa = 5.77 DD40 pKa = 6.03 LDD42 pKa = 6.35 DD43 pKa = 5.74 PDD45 pKa = 6.08 DD46 pKa = 4.73 DD47 pKa = 5.89 GYY49 pKa = 11.04 INSSDD54 pKa = 4.42 KK55 pKa = 11.2 EE56 pKa = 4.22 DD57 pKa = 3.68 ANKK60 pKa = 10.54 DD61 pKa = 3.34 AEE63 pKa = 4.48 KK64 pKa = 10.96 DD65 pKa = 3.5 NKK67 pKa = 10.3 FYY69 pKa = 11.44
Molecular weight: 8.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.726
IPC2_protein 3.643
IPC_protein 3.656
Toseland 3.414
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.694
Rodwell 3.478
Grimsley 3.325
Solomon 3.668
Lehninger 3.63
Nozaki 3.808
DTASelect 4.139
Thurlkill 3.503
EMBOSS 3.694
Sillero 3.795
Patrickios 1.888
IPC_peptide 3.656
IPC2_peptide 3.757
IPC2.peptide.svr19 3.732
Protein with the highest isoelectric point:
>tr|A0A553HYA3|A0A553HYA3_9PEZI Uncharacterized protein OS=Xylaria flabelliformis OX=2512241 GN=FHL15_006067 PE=4 SV=1
MM1 pKa = 7.31 TSSTPPLLQRR11 pKa = 11.84 PRR13 pKa = 11.84 AHH15 pKa = 7.29 HH16 pKa = 6.56 ARR18 pKa = 11.84 LDD20 pKa = 3.7 KK21 pKa = 10.61 TIPPALRR28 pKa = 11.84 PVIRR32 pKa = 11.84 AYY34 pKa = 10.9 VLGYY38 pKa = 10.6 ASAVAPRR45 pKa = 11.84 VLTLVLQYY53 pKa = 10.12 GARR56 pKa = 11.84 WRR58 pKa = 11.84 RR59 pKa = 11.84 RR60 pKa = 11.84 KK61 pKa = 10.02 SRR63 pKa = 11.84 GPVIVNGTQPQDD75 pKa = 3.26 SFVASLRR82 pKa = 11.84 RR83 pKa = 11.84 ILSGGLDD90 pKa = 3.52 PQRR93 pKa = 11.84 FPTFCATLIGGTTLLDD109 pKa = 3.44 VLVRR113 pKa = 11.84 RR114 pKa = 11.84 LMKK117 pKa = 10.09 RR118 pKa = 11.84 WKK120 pKa = 8.62 PQLSGIVRR128 pKa = 11.84 RR129 pKa = 11.84 RR130 pKa = 11.84 LSIWISSFIAGWLSLRR146 pKa = 11.84 LLQSKK151 pKa = 8.0 KK152 pKa = 8.58 TNAFAEE158 pKa = 4.78 TKK160 pKa = 10.33 AVNDD164 pKa = 4.51 DD165 pKa = 3.39 KK166 pKa = 11.61 QKK168 pKa = 8.63 TIRR171 pKa = 11.84 YY172 pKa = 8.75 AGRR175 pKa = 11.84 TLDD178 pKa = 3.65 LTLFAATRR186 pKa = 11.84 AVDD189 pKa = 3.8 VVVGEE194 pKa = 4.52 LWSQRR199 pKa = 11.84 RR200 pKa = 11.84 QRR202 pKa = 11.84 RR203 pKa = 11.84 VAADD207 pKa = 2.97 QWSWFDD213 pKa = 3.65 SFISKK218 pKa = 9.03 MADD221 pKa = 3.01 PSIFATSCALIMWAWFYY238 pKa = 10.07 TPCALPKK245 pKa = 10.33 SYY247 pKa = 11.31 NKK249 pKa = 9.45 WISSAAAVDD258 pKa = 3.61 SRR260 pKa = 11.84 LIEE263 pKa = 4.0 ALRR266 pKa = 11.84 RR267 pKa = 11.84 CHH269 pKa = 7.1 KK270 pKa = 10.93 GEE272 pKa = 3.76 LRR274 pKa = 11.84 YY275 pKa = 10.49 GEE277 pKa = 4.22 EE278 pKa = 3.97 TGQAPLLQSMCSDD291 pKa = 3.73 YY292 pKa = 11.04 KK293 pKa = 10.81 WPLEE297 pKa = 4.12 WGDD300 pKa = 3.63 PATTVPFPCEE310 pKa = 3.72 IVHH313 pKa = 6.09 MGCGPSCEE321 pKa = 3.93 YY322 pKa = 10.24 HH323 pKa = 5.61 ALYY326 pKa = 10.66 RR327 pKa = 11.84 FFRR330 pKa = 11.84 SFKK333 pKa = 10.01 WSMATYY339 pKa = 10.7 LPLNLLARR347 pKa = 11.84 KK348 pKa = 9.47 KK349 pKa = 10.36 DD350 pKa = 3.47 LQGFRR355 pKa = 11.84 KK356 pKa = 9.98 ALTSAARR363 pKa = 11.84 SSSFLATFITLFYY376 pKa = 10.98 YY377 pKa = 8.72 GVCLARR383 pKa = 11.84 TRR385 pKa = 11.84 LGPRR389 pKa = 11.84 LLGKK393 pKa = 8.49 HH394 pKa = 4.43 TASRR398 pKa = 11.84 QKK400 pKa = 10.09 IDD402 pKa = 3.35 AGVCVGCGCFMCGWSIFFEE421 pKa = 4.25 AASRR425 pKa = 11.84 RR426 pKa = 11.84 KK427 pKa = 10.55 DD428 pKa = 3.1 MALFVAPRR436 pKa = 11.84 ALATLLPRR444 pKa = 11.84 RR445 pKa = 11.84 YY446 pKa = 9.14 PLNKK450 pKa = 8.59 QWRR453 pKa = 11.84 EE454 pKa = 3.67 TFVFAFSTAIVFTCVRR470 pKa = 11.84 EE471 pKa = 3.69 NRR473 pKa = 11.84 ARR475 pKa = 11.84 VRR477 pKa = 11.84 GVLGNILGSVLEE489 pKa = 4.41 TT490 pKa = 3.81
Molecular weight: 55.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 9.692
IPC_protein 10.526
Toseland 10.628
ProMoST 10.35
Dawson 10.745
Bjellqvist 10.482
Wikipedia 10.965
Rodwell 10.935
Grimsley 10.804
Solomon 10.847
Lehninger 10.804
Nozaki 10.643
DTASelect 10.452
Thurlkill 10.643
EMBOSS 11.023
Sillero 10.672
Patrickios 10.599
IPC_peptide 10.847
IPC2_peptide 9.706
IPC2.peptide.svr19 8.507
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11403
0
11403
5968531
57
6921
523.4
57.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.491 ± 0.02
1.207 ± 0.007
5.83 ± 0.015
6.072 ± 0.02
3.668 ± 0.013
6.744 ± 0.02
2.416 ± 0.009
5.097 ± 0.015
4.774 ± 0.015
8.893 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.069 ± 0.007
3.848 ± 0.012
5.981 ± 0.023
3.971 ± 0.014
6.146 ± 0.02
8.292 ± 0.025
6.137 ± 0.017
6.069 ± 0.016
1.472 ± 0.008
2.821 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here