Capybara microvirus Cap1_SP_135
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W7Y1|A0A4P8W7Y1_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_135 OX=2585384 PE=4 SV=1
MM1 pKa = 7.6 LFNLKK6 pKa = 10.21 YY7 pKa = 10.6 YY8 pKa = 10.05 NKK10 pKa = 10.12 CEE12 pKa = 4.21 NPDD15 pKa = 3.92 FPPLLVPQKK24 pKa = 9.51 TIVEE28 pKa = 4.22 GSEE31 pKa = 3.61 EE32 pKa = 4.59 VIFLEE37 pKa = 5.25 HH38 pKa = 6.5 VPQNDD43 pKa = 4.06 FPSDD47 pKa = 3.48 MYY49 pKa = 11.39 DD50 pKa = 3.52 LQNLKK55 pKa = 10.53 SAGISPTQFRR65 pKa = 11.84 GGSVPSTSRR74 pKa = 11.84 LSDD77 pKa = 3.19 QSKK80 pKa = 11.05 ADD82 pKa = 4.22 AIVKK86 pKa = 10.36 NIVNALNTQEE96 pKa = 4.57 NEE98 pKa = 3.83
Molecular weight: 10.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.682
IPC2_protein 4.711
IPC_protein 4.533
Toseland 4.393
ProMoST 4.546
Dawson 4.495
Bjellqvist 4.711
Wikipedia 4.368
Rodwell 4.38
Grimsley 4.304
Solomon 4.482
Lehninger 4.431
Nozaki 4.609
DTASelect 4.749
Thurlkill 4.393
EMBOSS 4.38
Sillero 4.647
Patrickios 3.948
IPC_peptide 4.482
IPC2_peptide 4.647
IPC2.peptide.svr19 4.585
Protein with the highest isoelectric point:
>tr|A0A4P8W5D4|A0A4P8W5D4_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_135 OX=2585384 PE=4 SV=1
MM1 pKa = 7.53 SEE3 pKa = 4.22 ANRR6 pKa = 11.84 PLPAAGEE13 pKa = 4.29 TQAFFAVRR21 pKa = 11.84 VGLPVVHH28 pKa = 6.79 RR29 pKa = 11.84 KK30 pKa = 9.07 KK31 pKa = 10.62 RR32 pKa = 11.84 QTPKK36 pKa = 10.96 YY37 pKa = 10.46 LFALCDD43 pKa = 3.55 GMRR46 pKa = 11.84 TFCEE50 pKa = 4.2 LFRR53 pKa = 11.84 LEE55 pKa = 5.28 LNQSNCDD62 pKa = 3.08 ISQKK66 pKa = 7.39 TTVLTAVTNKK76 pKa = 10.27
Molecular weight: 8.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.957
IPC2_protein 8.741
IPC_protein 8.799
Toseland 9.589
ProMoST 9.487
Dawson 9.765
Bjellqvist 9.575
Wikipedia 9.867
Rodwell 10.175
Grimsley 9.75
Solomon 9.897
Lehninger 9.897
Nozaki 9.882
DTASelect 9.458
Thurlkill 9.677
EMBOSS 9.999
Sillero 9.809
Patrickios 10.16
IPC_peptide 9.897
IPC2_peptide 8.58
IPC2.peptide.svr19 7.708
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1658
76
581
331.6
38.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.031 ± 0.951
1.267 ± 0.422
6.514 ± 0.864
6.092 ± 0.999
5.187 ± 1.362
5.609 ± 0.795
1.628 ± 0.402
5.609 ± 0.764
6.936 ± 0.715
7.961 ± 0.569
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.473 ± 0.405
6.634 ± 0.844
3.739 ± 0.572
5.368 ± 0.933
4.946 ± 0.598
6.454 ± 0.364
5.247 ± 0.731
5.368 ± 0.3
0.905 ± 0.158
6.031 ± 1.229
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here