Drosophila albomicans (Fruit fly)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha;

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19749 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6P8XWL3|A0A6P8XWL3_DROAB carcinine transporter OS=Drosophila albomicans OX=7291 GN=LOC117575549 PE=4 SV=1
MM1 pKa = 8.24DD2 pKa = 3.65STFGSVFLFLTDD14 pKa = 4.45LAWKK18 pKa = 10.11SRR20 pKa = 11.84LTIPVLVTTAFLVMDD35 pKa = 4.11IRR37 pKa = 11.84LQIEE41 pKa = 4.02INIQTGQVNASDD53 pKa = 4.38LDD55 pKa = 3.7EE56 pKa = 6.61SEE58 pKa = 5.43DD59 pKa = 4.31DD60 pKa = 4.0GFEE63 pKa = 3.92EE64 pKa = 4.35HH65 pKa = 6.61LAAHH69 pKa = 6.92GVHH72 pKa = 6.74HH73 pKa = 6.91MDD75 pKa = 5.44DD76 pKa = 3.88DD77 pKa = 4.42SEE79 pKa = 5.55AYY81 pKa = 10.53SDD83 pKa = 5.62DD84 pKa = 3.71LEE86 pKa = 4.79EE87 pKa = 4.46YY88 pKa = 10.51NSEE91 pKa = 4.1YY92 pKa = 10.99TNGDD96 pKa = 3.46GNSSSRR102 pKa = 11.84SNSDD106 pKa = 3.02AASHH110 pKa = 5.73YY111 pKa = 10.75SAGIYY116 pKa = 10.28DD117 pKa = 4.97DD118 pKa = 5.49LGSDD122 pKa = 3.42YY123 pKa = 11.25DD124 pKa = 4.39AEE126 pKa = 4.62NEE128 pKa = 4.16LEE130 pKa = 4.99DD131 pKa = 4.53YY132 pKa = 10.63YY133 pKa = 11.92ALLTRR138 pKa = 11.84PPRR141 pKa = 11.84VV142 pKa = 3.15

Molecular weight: 15.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6P8XLG2|A0A6P8XLG2_DROAB beclin-1-like protein OS=Drosophila albomicans OX=7291 GN=LOC117574179 PE=3 SV=1
MM1 pKa = 7.51KK2 pKa = 10.47LLVALTVLVTVALAAPQFNSGARR25 pKa = 11.84PPSQAGGGGIIGRR38 pKa = 11.84RR39 pKa = 11.84PGGGVNGNRR48 pKa = 11.84GVPGGSPGGGPRR60 pKa = 11.84GGPGGGTGGGFGPGGGGFGGGFGPGGGGFGGGFGPGGGGFGGGFGPGGGGFGGGFGPGGGGFGGGPGGGFGHH132 pKa = 7.39RR133 pKa = 11.84GGNSGGASSASASASASTSGGGGRR157 pKa = 11.84GG158 pKa = 3.23

Molecular weight: 13.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13496

6253

19749

14002934

32

18460

709.0

78.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.798 ± 0.021

1.734 ± 0.02

5.265 ± 0.014

6.505 ± 0.028

3.242 ± 0.015

5.867 ± 0.028

2.622 ± 0.009

4.885 ± 0.015

5.499 ± 0.021

8.934 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.239 ± 0.009

5.043 ± 0.015

5.25 ± 0.024

5.839 ± 0.03

5.334 ± 0.014

8.402 ± 0.03

6.068 ± 0.022

5.708 ± 0.015

0.925 ± 0.006

2.836 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski