Firmicutes bacterium CAG:475

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1270 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7BTX1|R7BTX1_9FIRM 30S ribosomal protein S11 OS=Firmicutes bacterium CAG:475 OX=1263026 GN=rpsK PE=3 SV=1
MM1 pKa = 7.92ADD3 pKa = 5.09FFDD6 pKa = 4.78DD7 pKa = 3.91QNNMTEE13 pKa = 4.5DD14 pKa = 3.96DD15 pKa = 5.71DD16 pKa = 6.58IITLYY21 pKa = 11.29NEE23 pKa = 4.22VADD26 pKa = 3.94KK27 pKa = 11.59DD28 pKa = 3.45EE29 pKa = 4.9DD30 pKa = 4.63FYY32 pKa = 11.83HH33 pKa = 7.49LATLDD38 pKa = 4.02VDD40 pKa = 5.0DD41 pKa = 4.45KK42 pKa = 10.97WFVVLKK48 pKa = 10.15PVEE51 pKa = 4.18KK52 pKa = 10.64LDD54 pKa = 6.11DD55 pKa = 3.8IDD57 pKa = 3.87EE58 pKa = 4.89DD59 pKa = 3.74EE60 pKa = 4.25VLIYY64 pKa = 10.33EE65 pKa = 4.16IAEE68 pKa = 4.22NEE70 pKa = 4.24DD71 pKa = 3.39GNDD74 pKa = 3.13VFKK77 pKa = 11.01AIEE80 pKa = 4.74DD81 pKa = 3.79DD82 pKa = 4.4ALLQRR87 pKa = 11.84VFDD90 pKa = 3.74EE91 pKa = 5.66FMAEE95 pKa = 4.04VEE97 pKa = 4.38KK98 pKa = 11.01MEE100 pKa = 4.58GGCDD104 pKa = 3.42CGEE107 pKa = 4.74DD108 pKa = 3.68GCHH111 pKa = 7.35DD112 pKa = 4.27GNCSCCHH119 pKa = 5.99HH120 pKa = 6.87

Molecular weight: 13.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7BTF4|R7BTF4_9FIRM Uncharacterized protein OS=Firmicutes bacterium CAG:475 OX=1263026 GN=BN674_00266 PE=4 SV=1
MM1 pKa = 7.38SRR3 pKa = 11.84VCSVCGKK10 pKa = 10.7GRR12 pKa = 11.84MSGNAVSHH20 pKa = 6.07SNRR23 pKa = 11.84KK24 pKa = 6.7TRR26 pKa = 11.84RR27 pKa = 11.84SWAPNVQKK35 pKa = 11.13VKK37 pKa = 10.94VNTPTGGVEE46 pKa = 3.82DD47 pKa = 4.51TYY49 pKa = 11.78VCTRR53 pKa = 11.84CLRR56 pKa = 11.84SGKK59 pKa = 9.0VDD61 pKa = 3.39RR62 pKa = 11.84AA63 pKa = 3.5

Molecular weight: 6.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1270

0

1270

422948

38

3105

333.0

37.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.179 ± 0.065

1.747 ± 0.037

6.397 ± 0.06

6.572 ± 0.075

4.551 ± 0.057

6.907 ± 0.059

1.517 ± 0.031

6.944 ± 0.056

7.678 ± 0.062

8.415 ± 0.081

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.558 ± 0.039

4.705 ± 0.048

2.979 ± 0.04

2.846 ± 0.036

3.904 ± 0.054

6.013 ± 0.06

5.458 ± 0.074

8.04 ± 0.064

0.711 ± 0.021

3.876 ± 0.06
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski