Veillonella sp. DORA_B_18_19_23
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 870 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W1UCL2|W1UCL2_9FIRM Transporter major facilitator family protein OS=Veillonella sp. DORA_B_18_19_23 OX=1403933 GN=Q621_VSBC00505G0002 PE=4 SV=1
MM1 pKa = 7.4 GLIANYY7 pKa = 10.53 SYY9 pKa = 11.49 LSDD12 pKa = 3.86 EE13 pKa = 4.3 NLKK16 pKa = 9.92 EE17 pKa = 4.08 LKK19 pKa = 10.53 SLGSSEE25 pKa = 4.48 EE26 pKa = 4.34 DD27 pKa = 3.18 IIDD30 pKa = 4.21 SVEE33 pKa = 3.86 DD34 pKa = 3.49 WSEE37 pKa = 3.83 EE38 pKa = 4.15 DD39 pKa = 3.65 VLLDD43 pKa = 3.85 IDD45 pKa = 4.53 KK46 pKa = 9.81 MWDD49 pKa = 3.22 VLHH52 pKa = 6.75 FVLTGVGTEE61 pKa = 4.47 HH62 pKa = 7.63 RR63 pKa = 11.84 IDD65 pKa = 5.16 DD66 pKa = 4.33 NPLSQAVLGVTPIEE80 pKa = 5.15 DD81 pKa = 3.04 ISEE84 pKa = 3.93 YY85 pKa = 9.6 MAYY88 pKa = 9.03 TEE90 pKa = 4.71 HH91 pKa = 6.91 SQIADD96 pKa = 2.9 IVTALDD102 pKa = 3.53 EE103 pKa = 4.43 FDD105 pKa = 4.26 IEE107 pKa = 4.37 QALEE111 pKa = 4.24 TFDD114 pKa = 4.73 MMACKK119 pKa = 9.7 EE120 pKa = 4.12 AEE122 pKa = 4.53 LYY124 pKa = 9.78 PNIWDD129 pKa = 3.84 YY130 pKa = 12.13 DD131 pKa = 3.9 DD132 pKa = 5.0 EE133 pKa = 6.3 KK134 pKa = 11.66 DD135 pKa = 4.19 EE136 pKa = 4.85 IIEE139 pKa = 4.54 EE140 pKa = 3.83 ILDD143 pKa = 3.75 YY144 pKa = 10.71 FEE146 pKa = 5.03 HH147 pKa = 6.35 MKK149 pKa = 10.88 GFYY152 pKa = 10.32 KK153 pKa = 10.39 KK154 pKa = 10.56 VLASNGNVLITIYY167 pKa = 11.05
Molecular weight: 19.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.743
IPC2_protein 3.795
IPC_protein 3.783
Toseland 3.579
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.668
Rodwell 3.617
Grimsley 3.49
Solomon 3.757
Lehninger 3.706
Nozaki 3.872
DTASelect 4.075
Thurlkill 3.63
EMBOSS 3.681
Sillero 3.91
Patrickios 1.863
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.795
Protein with the highest isoelectric point:
>tr|W1UII5|W1UII5_9FIRM Transcriptional regulator (Fragment) OS=Veillonella sp. DORA_B_18_19_23 OX=1403933 GN=Q621_VSBC00398G0003 PE=4 SV=1
GG1 pKa = 7.39 RR2 pKa = 11.84 LDD4 pKa = 4.03 ADD6 pKa = 3.59 GSGDD10 pKa = 3.77 SRR12 pKa = 11.84 GLADD16 pKa = 3.74 RR17 pKa = 11.84 AAGVRR22 pKa = 11.84 TQGKK26 pKa = 9.65 RR27 pKa = 11.84 GLKK30 pKa = 9.99 GADD33 pKa = 2.84 GGRR36 pKa = 11.84 RR37 pKa = 11.84 STRR40 pKa = 11.84 GAARR44 pKa = 11.84 DD45 pKa = 3.68 TSQVPGVGGAVEE57 pKa = 4.38 GRR59 pKa = 11.84 MLGRR63 pKa = 11.84 ATHH66 pKa = 6.52 GEE68 pKa = 4.17 LIEE71 pKa = 4.12 VRR73 pKa = 11.84 LAQDD77 pKa = 3.29 RR78 pKa = 11.84 HH79 pKa = 5.59 AGRR82 pKa = 11.84 TQVCHH87 pKa = 6.19 GGGVARR93 pKa = 11.84 GLFVGILVTAISLFFVPFQVHH114 pKa = 4.27 SWVFVALTLVLTAVLFSLAGLLNGVFAKK142 pKa = 10.19 TFDD145 pKa = 5.28 DD146 pKa = 3.59 ISLVPTFVLTPLTYY160 pKa = 10.65 LGGVFYY166 pKa = 10.82 SLTLLPPP173 pKa = 4.35
Molecular weight: 18.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.344
IPC2_protein 9.443
IPC_protein 10.452
Toseland 10.555
ProMoST 10.365
Dawson 10.657
Bjellqvist 10.423
Wikipedia 10.921
Rodwell 10.687
Grimsley 10.716
Solomon 10.833
Lehninger 10.789
Nozaki 10.555
DTASelect 10.423
Thurlkill 10.57
EMBOSS 10.979
Sillero 10.599
Patrickios 10.467
IPC_peptide 10.833
IPC2_peptide 9.545
IPC2.peptide.svr19 8.665
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
870
0
870
178876
20
2384
205.6
22.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.582 ± 0.128
1.03 ± 0.039
5.828 ± 0.091
6.194 ± 0.106
3.862 ± 0.099
7.286 ± 0.135
2.173 ± 0.054
7.6 ± 0.093
6.33 ± 0.091
9.072 ± 0.136
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.714 ± 0.05
5.135 ± 0.128
3.478 ± 0.069
3.349 ± 0.061
4.04 ± 0.075
6.067 ± 0.102
6.077 ± 0.115
7.254 ± 0.084
0.949 ± 0.034
3.882 ± 0.085
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here