Flavobacterium sp. FPG59

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium; unclassified Flavobacterium

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3173 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A243SC85|A0A243SC85_9FLAO Uncharacterized protein (Fragment) OS=Flavobacterium sp. FPG59 OX=1929267 GN=FPG59_15490 PE=4 SV=1
MM1 pKa = 7.6NIQLPSFIFKK11 pKa = 10.39VPRR14 pKa = 11.84FLFLFWSLYY23 pKa = 7.75TVNSSAQVTANTNPSNAQINLALQGPGIVITGGTLVGAQTDD64 pKa = 4.35GAILRR69 pKa = 11.84SNQVATFTNGITGAGLGFPSGAYY92 pKa = 9.93FSTGNTTLEE101 pKa = 3.94LTNRR105 pKa = 11.84NTVIQQSAFPAAGATLSDD123 pKa = 4.44ADD125 pKa = 5.11LSTIDD130 pKa = 3.46PTATRR135 pKa = 11.84DD136 pKa = 3.32LVAYY140 pKa = 7.36TFTITLGPTITGLRR154 pKa = 11.84IGYY157 pKa = 9.14HH158 pKa = 6.12FGSEE162 pKa = 4.36EE163 pKa = 3.97YY164 pKa = 9.22PDD166 pKa = 3.74YY167 pKa = 11.35VGTVFDD173 pKa = 4.44DD174 pKa = 3.69AFGFFISGPGITGTRR189 pKa = 11.84NLATTPAGSPTSINKK204 pKa = 9.54INGGFPGSVAGATPVAAYY222 pKa = 10.2DD223 pKa = 3.87GTQSALYY230 pKa = 9.43INNGHH235 pKa = 5.43TTTVSGGAYY244 pKa = 9.25VANTDD249 pKa = 3.83PQPGPFPIFTEE260 pKa = 4.57FNGVTRR266 pKa = 11.84FITRR270 pKa = 11.84TVNNLTPGGTYY281 pKa = 7.71TFKK284 pKa = 11.21VVIADD289 pKa = 3.75ASDD292 pKa = 4.05DD293 pKa = 3.89FLDD296 pKa = 4.28SGVFLNLIEE305 pKa = 4.66GLTNANLQISKK316 pKa = 8.65TVNNPTPIVGSNVTFTLTASNLGSGAATGVNVTDD350 pKa = 4.61ILPAGYY356 pKa = 7.92TFDD359 pKa = 3.55SALPSVGTYY368 pKa = 11.24DD369 pKa = 3.25NTTGLWSIGDD379 pKa = 3.85LANGGNATLSIVARR393 pKa = 11.84VNASASYY400 pKa = 10.92INTATIAGNEE410 pKa = 4.01TDD412 pKa = 4.48PVLGNNTSTASIAIQPDD429 pKa = 3.32SDD431 pKa = 3.79NDD433 pKa = 4.02GVGDD437 pKa = 4.28FTDD440 pKa = 5.44LDD442 pKa = 4.45DD443 pKa = 6.1DD444 pKa = 4.25NDD446 pKa = 4.7GIRR449 pKa = 11.84DD450 pKa = 3.51IVEE453 pKa = 4.24DD454 pKa = 4.27TNCASGPFVVEE465 pKa = 4.29NILNEE470 pKa = 4.06VFEE473 pKa = 4.57TGTVGFPVPKK483 pKa = 9.83GDD485 pKa = 3.56LVGVPGIVGLLEE497 pKa = 4.87AYY499 pKa = 10.18NSNPGNSSTTNVGEE513 pKa = 4.09YY514 pKa = 10.4SSVVGFDD521 pKa = 3.42GNLTKK526 pKa = 10.7VINANGGGIVDD537 pKa = 4.01QLQMSSFVVKK547 pKa = 10.75KK548 pKa = 10.78NIDD551 pKa = 3.64LLAGNIITVSADD563 pKa = 3.22FSLDD567 pKa = 3.19MAAGGPSNEE576 pKa = 3.92YY577 pKa = 10.78GIALGAGGQDD587 pKa = 3.95PIWRR591 pKa = 11.84DD592 pKa = 3.74DD593 pKa = 3.66VPGVPDD599 pKa = 3.85GVFLYY604 pKa = 10.46GHH606 pKa = 6.49GVSLIRR612 pKa = 11.84EE613 pKa = 4.39PNSSGPTFTAPSRR626 pKa = 11.84VTGWFRR632 pKa = 11.84QKK634 pKa = 9.32STYY637 pKa = 10.23YY638 pKa = 10.05VANNGSGVLHH648 pKa = 7.0LYY650 pKa = 10.68ANNEE654 pKa = 4.09GYY656 pKa = 10.4QYY658 pKa = 11.13GPTGIPTSAPIIANGINFGPASNYY682 pKa = 8.76PWLNNASLSVSVDD695 pKa = 3.22QYY697 pKa = 11.44VDD699 pKa = 3.57NIVINVNHH707 pKa = 7.26CDD709 pKa = 3.62FDD711 pKa = 5.97GDD713 pKa = 4.17GVQNSFDD720 pKa = 4.54LDD722 pKa = 3.86SDD724 pKa = 3.87NDD726 pKa = 3.96GCSDD730 pKa = 3.79SNEE733 pKa = 4.29YY734 pKa = 10.33YY735 pKa = 11.34NNATSAASGQQFGQTGGAVAPVNANGTVNLPAATYY770 pKa = 8.39TGSYY774 pKa = 11.06ANATSVGTSSSIATQPVNRR793 pKa = 11.84IINAGTNTTFSVAAAGGSGVTQYY816 pKa = 9.94QWQVDD821 pKa = 4.01SGSGFTDD828 pKa = 3.47ISNGAVYY835 pKa = 11.1NNATTNTLTLTSVPATFNGYY855 pKa = 7.57VYY857 pKa = 10.43RR858 pKa = 11.84VIIRR862 pKa = 11.84EE863 pKa = 4.31SNFVCSSVVSAEE875 pKa = 3.81RR876 pKa = 11.84TLTVFPAPVVTIADD890 pKa = 3.52ASITEE895 pKa = 4.23GGNLSFPVTLSNPSATDD912 pKa = 3.01ITVTLGFTNVTTANGDD928 pKa = 3.71YY929 pKa = 7.76TTVPVTVTFLAGATTATATVPTTADD954 pKa = 3.55TIDD957 pKa = 3.89EE958 pKa = 4.37LDD960 pKa = 3.4EE961 pKa = 4.48TFTVAITSTTGTVGSTTDD979 pKa = 3.24TATGTINDD987 pKa = 4.83DD988 pKa = 3.86DD989 pKa = 4.29NAPTVASISPSNAA1002 pKa = 2.79

Molecular weight: 103.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2C9ZPF7|A0A2C9ZPF7_9FLAO Alkyl hydroperoxide reductase OS=Flavobacterium sp. FPG59 OX=1929267 GN=FPG59_11905 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.91HH17 pKa = 4.39GFMDD21 pKa = 4.5RR22 pKa = 11.84MASANGRR29 pKa = 11.84KK30 pKa = 9.04VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.19GRR40 pKa = 11.84HH41 pKa = 5.41KK42 pKa = 10.14LTVSSEE48 pKa = 3.92PRR50 pKa = 11.84HH51 pKa = 5.77KK52 pKa = 10.61KK53 pKa = 9.84

Molecular weight: 6.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3173

0

3173

1053902

25

2925

332.1

37.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.61 ± 0.052

0.788 ± 0.015

5.221 ± 0.028

6.328 ± 0.046

5.416 ± 0.037

6.137 ± 0.042

1.674 ± 0.021

8.126 ± 0.041

8.013 ± 0.048

9.293 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.202 ± 0.024

6.356 ± 0.052

3.32 ± 0.025

3.635 ± 0.026

3.144 ± 0.031

6.468 ± 0.038

6.055 ± 0.053

6.323 ± 0.035

0.972 ± 0.015

3.918 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski