Satellite tobacco necrosis virus 1
Average proteome isoelectric point is 9.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P03606|CAPSD_STNV1 Capsid protein OS=Satellite tobacco necrosis virus 1 OX=12445 PE=1 SV=1
MM1 pKa = 7.97 AKK3 pKa = 9.46 QQNNRR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 8.68 SATMRR15 pKa = 11.84 AVKK18 pKa = 10.47 RR19 pKa = 11.84 MINTHH24 pKa = 6.06 LEE26 pKa = 4.02 HH27 pKa = 7.35 KK28 pKa = 10.31 RR29 pKa = 11.84 FALINSGNTNATAGTVQNLSNGIIQGDD56 pKa = 4.5 DD57 pKa = 2.77 INQRR61 pKa = 11.84 SGDD64 pKa = 3.48 QVRR67 pKa = 11.84 IVSHH71 pKa = 6.34 KK72 pKa = 9.32 LHH74 pKa = 6.04 VRR76 pKa = 11.84 GTAITVSQTFRR87 pKa = 11.84 FIWFRR92 pKa = 11.84 DD93 pKa = 3.12 NMNRR97 pKa = 11.84 GTTPTVLEE105 pKa = 4.33 VLNTANFMSQYY116 pKa = 11.19 NPITLQQKK124 pKa = 9.91 RR125 pKa = 11.84 FTILKK130 pKa = 10.13 DD131 pKa = 3.26 VTLNCSLTGEE141 pKa = 4.4 SIKK144 pKa = 10.91 DD145 pKa = 3.58 RR146 pKa = 11.84 IINLPGQLVNYY157 pKa = 9.98 NGATAVAASNGPGAIFMLQIGDD179 pKa = 4.24 SLVGLWDD186 pKa = 3.43 SSYY189 pKa = 10.94 EE190 pKa = 4.24 AVYY193 pKa = 10.66 TDD195 pKa = 3.26 AA196 pKa = 6.56
Molecular weight: 21.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.192
IPC2_protein 9.355
IPC_protein 9.531
Toseland 10.16
ProMoST 9.809
Dawson 10.335
Bjellqvist 9.999
Wikipedia 10.496
Rodwell 10.687
Grimsley 10.394
Solomon 10.379
Lehninger 10.35
Nozaki 10.145
DTASelect 9.984
Thurlkill 10.189
EMBOSS 10.555
Sillero 10.248
Patrickios 10.394
IPC_peptide 10.379
IPC2_peptide 8.653
IPC2.peptide.svr19 8.405
Protein with the highest isoelectric point:
>sp|P03606|CAPSD_STNV1 Capsid protein OS=Satellite tobacco necrosis virus 1 OX=12445 PE=1 SV=1
MM1 pKa = 7.97 AKK3 pKa = 9.46 QQNNRR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 8.68 SATMRR15 pKa = 11.84 AVKK18 pKa = 10.47 RR19 pKa = 11.84 MINTHH24 pKa = 6.06 LEE26 pKa = 4.02 HH27 pKa = 7.35 KK28 pKa = 10.31 RR29 pKa = 11.84 FALINSGNTNATAGTVQNLSNGIIQGDD56 pKa = 4.5 DD57 pKa = 2.77 INQRR61 pKa = 11.84 SGDD64 pKa = 3.48 QVRR67 pKa = 11.84 IVSHH71 pKa = 6.34 KK72 pKa = 9.32 LHH74 pKa = 6.04 VRR76 pKa = 11.84 GTAITVSQTFRR87 pKa = 11.84 FIWFRR92 pKa = 11.84 DD93 pKa = 3.12 NMNRR97 pKa = 11.84 GTTPTVLEE105 pKa = 4.33 VLNTANFMSQYY116 pKa = 11.19 NPITLQQKK124 pKa = 9.91 RR125 pKa = 11.84 FTILKK130 pKa = 10.13 DD131 pKa = 3.26 VTLNCSLTGEE141 pKa = 4.4 SIKK144 pKa = 10.91 DD145 pKa = 3.58 RR146 pKa = 11.84 IINLPGQLVNYY157 pKa = 9.98 NGATAVAASNGPGAIFMLQIGDD179 pKa = 4.24 SLVGLWDD186 pKa = 3.43 SSYY189 pKa = 10.94 EE190 pKa = 4.24 AVYY193 pKa = 10.66 TDD195 pKa = 3.26 AA196 pKa = 6.56
Molecular weight: 21.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.192
IPC2_protein 9.355
IPC_protein 9.531
Toseland 10.16
ProMoST 9.809
Dawson 10.335
Bjellqvist 9.999
Wikipedia 10.496
Rodwell 10.687
Grimsley 10.394
Solomon 10.379
Lehninger 10.35
Nozaki 10.145
DTASelect 9.984
Thurlkill 10.189
EMBOSS 10.555
Sillero 10.248
Patrickios 10.394
IPC_peptide 10.379
IPC2_peptide 8.653
IPC2.peptide.svr19 8.405
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
196
196
196
196.0
21.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.653 ± 0.0
0.51 ± 0.0
4.592 ± 0.0
2.041 ± 0.0
3.571 ± 0.0
7.143 ± 0.0
2.041 ± 0.0
7.653 ± 0.0
4.082 ± 0.0
7.653 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.061 ± 0.0
9.694 ± 0.0
2.041 ± 0.0
6.122 ± 0.0
6.633 ± 0.0
6.633 ± 0.0
9.184 ± 0.0
6.633 ± 0.0
1.02 ± 0.0
2.041 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here