Brevibacterium phage AGM3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Agmunavirus; unclassified Agmunavirus

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D0KGV7|A0A7D0KGV7_9CAUD Uncharacterized protein OS=Brevibacterium phage AGM3 OX=2591420 GN=AGM3_0027 PE=4 SV=1
MM1 pKa = 7.24TLVKK5 pKa = 10.19GPNGIVVDD13 pKa = 4.1VASTVASGLIGGGYY27 pKa = 9.06VVAVDD32 pKa = 4.58SKK34 pKa = 11.67DD35 pKa = 3.51SGHH38 pKa = 6.51TPDD41 pKa = 3.18QTTDD45 pKa = 2.87VTAEE49 pKa = 4.28DD50 pKa = 4.19EE51 pKa = 4.61EE52 pKa = 4.54EE53 pKa = 4.01TAPAKK58 pKa = 10.12PAGNASKK65 pKa = 11.06GDD67 pKa = 3.45WEE69 pKa = 5.02AYY71 pKa = 10.04ALSQGFGEE79 pKa = 4.3EE80 pKa = 3.99QLDD83 pKa = 3.85GLKK86 pKa = 10.14QRR88 pKa = 11.84EE89 pKa = 4.13IRR91 pKa = 11.84DD92 pKa = 3.86LLDD95 pKa = 3.35EE96 pKa = 4.43

Molecular weight: 9.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D0GJ89|A0A7D0GJ89_9CAUD Uncharacterized protein OS=Brevibacterium phage AGM3 OX=2591420 GN=AGM3_0048 PE=4 SV=1
MM1 pKa = 7.53SIHH4 pKa = 6.5RR5 pKa = 11.84RR6 pKa = 11.84GGILKK11 pKa = 9.84HH12 pKa = 5.02FTLIEE17 pKa = 4.16VLSPEE22 pKa = 4.21HH23 pKa = 6.78GGLTRR28 pKa = 11.84AAFRR32 pKa = 11.84RR33 pKa = 11.84STALAALGEE42 pKa = 4.34RR43 pKa = 11.84VEE45 pKa = 4.29VLCFNLVPDD54 pKa = 4.71FSGLAEE60 pKa = 4.09HH61 pKa = 6.31VRR63 pKa = 11.84EE64 pKa = 4.0HH65 pKa = 8.3SEE67 pKa = 3.72IGDD70 pKa = 3.6VALSNFFHH78 pKa = 7.12ALAGSDD84 pKa = 3.7EE85 pKa = 5.43AITAQGARR93 pKa = 11.84HH94 pKa = 6.29ANLSTTPQQLRR105 pKa = 11.84PVDD108 pKa = 3.61GMTVSDD114 pKa = 4.3EE115 pKa = 4.26EE116 pKa = 4.67FDD118 pKa = 3.88TVTQSLRR125 pKa = 11.84LRR127 pKa = 11.84NYY129 pKa = 9.71VDD131 pKa = 3.47AKK133 pKa = 10.76GALTRR138 pKa = 11.84VDD140 pKa = 4.05FLDD143 pKa = 4.23RR144 pKa = 11.84YY145 pKa = 10.43GKK147 pKa = 10.62LFFVDD152 pKa = 3.65EE153 pKa = 4.66RR154 pKa = 11.84KK155 pKa = 6.97TTTGQARR162 pKa = 11.84RR163 pKa = 11.84MTLVDD168 pKa = 3.48RR169 pKa = 11.84RR170 pKa = 11.84TGNLAYY176 pKa = 10.22QGGYY180 pKa = 10.23GYY182 pKa = 9.59EE183 pKa = 3.8ASAARR188 pKa = 11.84TTSAPRR194 pKa = 11.84GRR196 pKa = 11.84KK197 pKa = 8.45VGRR200 pKa = 11.84HH201 pKa = 4.07VRR203 pKa = 11.84ACHH206 pKa = 5.99RR207 pKa = 11.84RR208 pKa = 11.84TSSICAEE215 pKa = 4.09PHH217 pKa = 6.29HH218 pKa = 7.53DD219 pKa = 5.22DD220 pKa = 4.32RR221 pKa = 11.84PPPPRR226 pKa = 11.84RR227 pKa = 11.84WGSVRR232 pKa = 11.84FWLKK236 pKa = 10.37LYY238 pKa = 10.36RR239 pKa = 11.84GIYY242 pKa = 9.14RR243 pKa = 11.84VRR245 pKa = 11.84NPARR249 pKa = 11.84TT250 pKa = 3.39

Molecular weight: 28.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

11578

34

2012

218.5

23.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.209 ± 0.547

0.967 ± 0.221

6.504 ± 0.398

6.08 ± 0.3

2.66 ± 0.206

8.698 ± 0.524

1.909 ± 0.219

4.526 ± 0.194

4.595 ± 0.401

7.376 ± 0.283

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.436 ± 0.206

3.455 ± 0.251

5.191 ± 0.597

3.714 ± 0.153

7.048 ± 0.489

6.746 ± 0.415

7.022 ± 0.255

6.711 ± 0.288

1.641 ± 0.174

2.513 ± 0.187
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski