Satellite panicum mosaic virus (SPMV)
Average proteome isoelectric point is 9.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q86994|Q86994_SPMV Uncharacterized protein OS=Satellite panicum mosaic virus OX=154834 PE=4 SV=1
MM1 pKa = 7.1 LRR3 pKa = 11.84 KK4 pKa = 9.15 WGNQEE9 pKa = 3.5 ATKK12 pKa = 10.1 PSQQRR17 pKa = 11.84 RR18 pKa = 11.84 PCVQPTRR25 pKa = 11.84 PEE27 pKa = 3.84 IYY29 pKa = 9.72 PEE31 pKa = 4.25 RR32 pKa = 11.84 GGPAAGSPFQWQCHH46 pKa = 4.54 FPGGRR51 pKa = 11.84 CVSPSS56 pKa = 2.96
Molecular weight: 6.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.217
IPC2_protein 9.063
IPC_protein 9.37
Toseland 9.838
ProMoST 9.589
Dawson 10.058
Bjellqvist 9.882
Wikipedia 10.218
Rodwell 10.262
Grimsley 10.087
Solomon 10.189
Lehninger 10.175
Nozaki 10.131
DTASelect 9.78
Thurlkill 9.94
EMBOSS 10.262
Sillero 10.072
Patrickios 10.292
IPC_peptide 10.189
IPC2_peptide 9.414
IPC2.peptide.svr19 7.81
Protein with the highest isoelectric point:
>tr|Q86994|Q86994_SPMV Uncharacterized protein OS=Satellite panicum mosaic virus OX=154834 PE=4 SV=1
MM1 pKa = 7.38 APKK4 pKa = 10.36 RR5 pKa = 11.84 SRR7 pKa = 11.84 RR8 pKa = 11.84 SNRR11 pKa = 11.84 RR12 pKa = 11.84 AGSRR16 pKa = 11.84 AAATSLVYY24 pKa = 8.6 DD25 pKa = 3.95 TCYY28 pKa = 9.99 VTLTEE33 pKa = 4.19 RR34 pKa = 11.84 ATTSFQRR41 pKa = 11.84 QSFPTLKK48 pKa = 10.78 GMGDD52 pKa = 3.22 RR53 pKa = 11.84 AFQVVAFTIQGVSAAPLMYY72 pKa = 10.23 NARR75 pKa = 11.84 LYY77 pKa = 11.27 NPGDD81 pKa = 3.7 TDD83 pKa = 3.87 SVHH86 pKa = 5.51 ATGVQLMGTVPRR98 pKa = 11.84 TVRR101 pKa = 11.84 LTPRR105 pKa = 11.84 VGQNNWFFGNTEE117 pKa = 3.77 EE118 pKa = 5.65 AEE120 pKa = 4.31 TILAIDD126 pKa = 4.27 GLVSTKK132 pKa = 10.34 GANAPSNTVIVTGCFRR148 pKa = 11.84 LAPSEE153 pKa = 4.14 LQSSS157 pKa = 3.98
Molecular weight: 16.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.312
IPC2_protein 9.472
IPC_protein 10.277
Toseland 10.072
ProMoST 9.955
Dawson 10.335
Bjellqvist 10.116
Wikipedia 10.584
Rodwell 10.409
Grimsley 10.423
Solomon 10.409
Lehninger 10.365
Nozaki 10.145
DTASelect 10.101
Thurlkill 10.175
EMBOSS 10.511
Sillero 10.262
Patrickios 9.97
IPC_peptide 10.409
IPC2_peptide 9.297
IPC2.peptide.svr19 8.429
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
213
56
157
106.5
11.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.39 ± 2.002
2.347 ± 1.494
2.347 ± 1.165
3.756 ± 0.795
4.225 ± 0.325
8.451 ± 1.124
0.939 ± 0.42
2.347 ± 0.279
2.347 ± 0.608
5.634 ± 1.91
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.347 ± 0.279
4.225 ± 1.211
7.981 ± 4.017
6.103 ± 2.289
9.39 ± 0.658
7.981 ± 0.416
9.39 ± 2.889
7.042 ± 1.723
1.408 ± 1.074
2.347 ± 0.279
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here