Lactococcus phage 49801

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8BKI1|A0A1P8BKI1_9CAUD Uncharacterized protein OS=Lactococcus phage 49801 OX=1868847 GN=DS49801_22 PE=4 SV=1
MM1 pKa = 7.39ILSEE5 pKa = 4.27IEE7 pKa = 4.01AVSPEE12 pKa = 4.07DD13 pKa = 3.31FVVFEE18 pKa = 5.64DD19 pKa = 3.94DD20 pKa = 3.16TPYY23 pKa = 11.05SYY25 pKa = 10.77TIDD28 pKa = 3.41RR29 pKa = 11.84GCRR32 pKa = 11.84EE33 pKa = 3.83NAIEE37 pKa = 4.64VDD39 pKa = 4.65DD40 pKa = 4.4FPKK43 pKa = 10.66LYY45 pKa = 9.59TAEE48 pKa = 3.92QMQEE52 pKa = 3.89YY53 pKa = 10.56AKK55 pKa = 10.64ANCWEE60 pKa = 4.57LINWYY65 pKa = 10.43VEE67 pKa = 3.97TTGDD71 pKa = 3.57VNHH74 pKa = 6.68AAEE77 pKa = 4.34MKK79 pKa = 9.86IWMDD83 pKa = 3.71DD84 pKa = 3.17EE85 pKa = 5.1FGGHH89 pKa = 5.75EE90 pKa = 4.15KK91 pKa = 10.79

Molecular weight: 10.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8BKF2|A0A1P8BKF2_9CAUD dUTP diphosphatase OS=Lactococcus phage 49801 OX=1868847 GN=DS49801_25 PE=4 SV=1
MM1 pKa = 7.62HH2 pKa = 7.57YY3 pKa = 9.71IPKK6 pKa = 10.05YY7 pKa = 9.07SRR9 pKa = 11.84EE10 pKa = 4.05RR11 pKa = 11.84QKK13 pKa = 10.94KK14 pKa = 8.58RR15 pKa = 11.84RR16 pKa = 11.84NNDD19 pKa = 3.27VIRR22 pKa = 11.84VTTSPFLTEE31 pKa = 3.67VHH33 pKa = 6.7SIADD37 pKa = 3.34ATVNIVRR44 pKa = 11.84NEE46 pKa = 3.75IKK48 pKa = 10.3KK49 pKa = 10.67ANYY52 pKa = 9.71QIGKK56 pKa = 9.61

Molecular weight: 6.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

10874

42

929

181.2

20.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.079 ± 0.368

0.543 ± 0.103

6.217 ± 0.215

7.624 ± 0.704

4.46 ± 0.192

6.217 ± 0.489

1.26 ± 0.15

6.603 ± 0.255

9.353 ± 0.449

8.093 ± 0.372

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.575 ± 0.135

6.189 ± 0.21

2.649 ± 0.162

4.304 ± 0.266

3.734 ± 0.266

6.447 ± 0.277

6.309 ± 0.331

5.959 ± 0.334

1.527 ± 0.22

3.862 ± 0.274
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski