Thermus phage phiOH3

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Paulinoviridae; Thomixvirus; Thermus virus OH3

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M5N6V9|A0A0M5N6V9_9VIRU Minor coat protein OS=Thermus phage phiOH3 OX=1968339 GN=VII PE=4 SV=1
MM1 pKa = 7.43SRR3 pKa = 11.84LALPILLVLLSLGWGGPALAQPQLYY28 pKa = 9.88RR29 pKa = 11.84DD30 pKa = 4.78AIRR33 pKa = 11.84SAVQDD38 pKa = 3.45MVVAEE43 pKa = 4.54VIRR46 pKa = 11.84WPDD49 pKa = 3.08SAVRR53 pKa = 11.84KK54 pKa = 9.25SATVGRR60 pKa = 11.84YY61 pKa = 7.17VRR63 pKa = 11.84WGGAIVLGSALVYY76 pKa = 9.11TALDD80 pKa = 3.25YY81 pKa = 10.78FYY83 pKa = 11.29NVLKK87 pKa = 10.73AQTGTSLDD95 pKa = 2.93RR96 pKa = 11.84WYY98 pKa = 10.45FWSNPYY104 pKa = 10.18VEE106 pKa = 4.75VGRR109 pKa = 11.84CIDD112 pKa = 3.37RR113 pKa = 11.84GSYY116 pKa = 10.46RR117 pKa = 11.84DD118 pKa = 4.79FIAVAYY124 pKa = 10.47VNGRR128 pKa = 11.84QVNFYY133 pKa = 9.34STSSYY138 pKa = 10.65GKK140 pKa = 9.84CDD142 pKa = 3.54VPSLQSFTEE151 pKa = 3.85HH152 pKa = 6.53WYY154 pKa = 9.51NWHH157 pKa = 6.52LVRR160 pKa = 11.84NYY162 pKa = 10.88SEE164 pKa = 4.26LQGVPTQYY172 pKa = 10.77VSPPPGTCPSGWTCIAVAPQLDD194 pKa = 4.49RR195 pKa = 11.84PPLPDD200 pKa = 3.39WLQSHH205 pKa = 7.6PDD207 pKa = 3.16AADD210 pKa = 3.53GVKK213 pKa = 10.1QAVTTYY219 pKa = 11.07LDD221 pKa = 3.8RR222 pKa = 11.84NPIGSPYY229 pKa = 10.58APYY232 pKa = 10.19PGVQLEE238 pKa = 4.97PIPNPNQWTDD248 pKa = 3.29NPFTRR253 pKa = 11.84PDD255 pKa = 3.46IDD257 pKa = 3.52TDD259 pKa = 3.77GDD261 pKa = 3.69GWPDD265 pKa = 3.31PVEE268 pKa = 3.51WRR270 pKa = 11.84EE271 pKa = 3.72ANRR274 pKa = 11.84RR275 pKa = 11.84GVPWPDD281 pKa = 5.02LINNPQAYY289 pKa = 7.88PDD291 pKa = 4.32PNGDD295 pKa = 3.86PDD297 pKa = 4.24GDD299 pKa = 4.04GYY301 pKa = 9.92TNLEE305 pKa = 4.17EE306 pKa = 4.4VQQGTDD312 pKa = 3.27PYY314 pKa = 11.06DD315 pKa = 3.7PASYY319 pKa = 9.78PVRR322 pKa = 11.84RR323 pKa = 11.84SPTSPWVDD331 pKa = 3.05TDD333 pKa = 3.34GDD335 pKa = 4.29GYY337 pKa = 11.18SDD339 pKa = 3.59EE340 pKa = 4.36EE341 pKa = 4.49EE342 pKa = 3.98IRR344 pKa = 11.84KK345 pKa = 7.8GTNPNDD351 pKa = 3.54PASYY355 pKa = 10.12PDD357 pKa = 4.25TPPEE361 pKa = 3.93QQPEE365 pKa = 4.26EE366 pKa = 4.51NPDD369 pKa = 3.22EE370 pKa = 4.5PQWPGGPPSGRR381 pKa = 11.84IDD383 pKa = 3.4PVQLPEE389 pKa = 3.98VEE391 pKa = 4.04QVEE394 pKa = 4.5RR395 pKa = 11.84EE396 pKa = 4.33KK397 pKa = 11.31LPEE400 pKa = 3.83WDD402 pKa = 3.96KK403 pKa = 11.71LNPLAEE409 pKa = 4.1AWRR412 pKa = 11.84QQVVDD417 pKa = 3.9RR418 pKa = 11.84VSQKK422 pKa = 10.05FAEE425 pKa = 4.35VQNILKK431 pKa = 10.46DD432 pKa = 3.55RR433 pKa = 11.84FPFGIIAAIRR443 pKa = 11.84QRR445 pKa = 11.84VSFADD450 pKa = 3.68AQCAIQLSLPPLGTLAVDD468 pKa = 3.84ICSTPVWQLATSFRR482 pKa = 11.84PVLAGLVWVAFGFAVIRR499 pKa = 11.84RR500 pKa = 11.84ALDD503 pKa = 3.3VQKK506 pKa = 11.3

Molecular weight: 56.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M4VAS8|A0A0M4VAS8_9VIRU Minor coat protein OS=Thermus phage phiOH3 OX=1968339 GN=VI PE=4 SV=1
MM1 pKa = 7.48EE2 pKa = 5.55FDD4 pKa = 3.5AGAIVSTVQNYY15 pKa = 9.28ILAIAGAGVALLGLTIGLSAAWRR38 pKa = 11.84YY39 pKa = 9.02AKK41 pKa = 10.47KK42 pKa = 10.14FLKK45 pKa = 10.68GG46 pKa = 3.23

Molecular weight: 4.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

1619

30

506

202.4

22.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.068 ± 1.185

0.556 ± 0.285

5.806 ± 0.911

5.188 ± 0.84

3.644 ± 0.477

7.844 ± 0.809

1.112 ± 0.272

3.83 ± 0.989

3.027 ± 0.592

10.377 ± 1.639

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.606 ± 0.466

2.347 ± 0.619

7.288 ± 1.56

3.582 ± 0.875

7.041 ± 1.002

5.683 ± 0.556

3.891 ± 0.53

9.574 ± 0.926

3.582 ± 0.426

3.953 ± 0.54
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski