Dimargaris cristalligena

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Zoopagomycota; Kickxellomycotina; Dimargaritomycetes; Dimargaritales; Dimargaritaceae; Dimargaris

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7376 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4Q0A2S5|A0A4Q0A2S5_9FUNG Chitin synthase (Fragment) OS=Dimargaris cristalligena OX=215637 GN=BJ085DRAFT_5961 PE=4 SV=1
MM1 pKa = 7.51KK2 pKa = 9.74PYY4 pKa = 10.5LVPITLLGLALACSVTGRR22 pKa = 11.84PQPADD27 pKa = 3.44LGEE30 pKa = 4.02VSGYY34 pKa = 7.42EE35 pKa = 3.97TQLGNNTSDD44 pKa = 3.35YY45 pKa = 11.23GNNSDD50 pKa = 6.33DD51 pKa = 5.35DD52 pKa = 5.1DD53 pKa = 5.16FDD55 pKa = 5.71NFDD58 pKa = 4.84DD59 pKa = 6.06DD60 pKa = 5.95FDD62 pKa = 5.4NFDD65 pKa = 5.55DD66 pKa = 6.39DD67 pKa = 6.42FDD69 pKa = 5.44DD70 pKa = 5.75FNDD73 pKa = 4.89DD74 pKa = 5.29FDD76 pKa = 6.57DD77 pKa = 4.84FDD79 pKa = 6.81DD80 pKa = 5.41SVAEE84 pKa = 4.13PPKK87 pKa = 10.49TRR89 pKa = 11.84APQNNPINRR98 pKa = 11.84LSFLPRR104 pKa = 11.84EE105 pKa = 4.07VFYY108 pKa = 11.47NVLDD112 pKa = 3.82YY113 pKa = 11.31LPPNDD118 pKa = 4.14RR119 pKa = 11.84YY120 pKa = 10.5EE121 pKa = 4.17LSEE124 pKa = 4.23VDD126 pKa = 4.68DD127 pKa = 4.17KK128 pKa = 12.13SNYY131 pKa = 9.74LSNLHH136 pKa = 6.49KK137 pKa = 8.47PTHH140 pKa = 6.51VILQVTRR147 pKa = 11.84PWSNYY152 pKa = 7.26VYY154 pKa = 10.25SYY156 pKa = 8.91STSMPIDD163 pKa = 3.17

Molecular weight:
18.64 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4Q0A0S3|A0A4Q0A0S3_9FUNG Phosphoinositide phospholipase C (Fragment) OS=Dimargaris cristalligena OX=215637 GN=BJ085DRAFT_16957 PE=4 SV=1
RR1 pKa = 7.89PYY3 pKa = 9.58ICAWSTCQKK12 pKa = 10.87AFARR16 pKa = 11.84KK17 pKa = 9.03SDD19 pKa = 3.46LMRR22 pKa = 11.84HH23 pKa = 5.04RR24 pKa = 11.84RR25 pKa = 11.84IHH27 pKa = 5.26TGEE30 pKa = 3.98RR31 pKa = 11.84PYY33 pKa = 11.06PCDD36 pKa = 3.17WPNCGKK42 pKa = 10.49RR43 pKa = 11.84FIQRR47 pKa = 11.84SALTVHH53 pKa = 5.9YY54 pKa = 7.38RR55 pKa = 11.84THH57 pKa = 5.66TGEE60 pKa = 4.33RR61 pKa = 11.84PHH63 pKa = 6.87TCDD66 pKa = 3.06FCNKK70 pKa = 9.29NFSDD74 pKa = 3.75SSSLARR80 pKa = 11.84HH81 pKa = 5.87RR82 pKa = 11.84RR83 pKa = 11.84THH85 pKa = 5.12TGKK88 pKa = 10.32RR89 pKa = 11.84PYY91 pKa = 10.95ACDD94 pKa = 3.18HH95 pKa = 6.65LGCGRR100 pKa = 11.84TFTRR104 pKa = 11.84RR105 pKa = 11.84TTLTKK110 pKa = 10.42HH111 pKa = 4.64QQ112 pKa = 3.89

Molecular weight:
13.23 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7376

0

7376

2791136

49

7319

378.4

41.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.382 ± 0.029

1.306 ± 0.012

5.455 ± 0.024

5.182 ± 0.03

3.772 ± 0.02

6.115 ± 0.027

2.763 ± 0.017

4.881 ± 0.026

4.002 ± 0.031

9.837 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.13 ± 0.013

3.827 ± 0.019

6.814 ± 0.05

4.49 ± 0.024

5.885 ± 0.024

8.169 ± 0.036

6.566 ± 0.024

6.164 ± 0.031

1.221 ± 0.011

3.04 ± 0.018

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski