Dimargaris cristalligena
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7376 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Q0A2S5|A0A4Q0A2S5_9FUNG Chitin synthase (Fragment) OS=Dimargaris cristalligena OX=215637 GN=BJ085DRAFT_5961 PE=4 SV=1
MM1 pKa = 7.51 KK2 pKa = 9.74 PYY4 pKa = 10.5 LVPITLLGLALACSVTGRR22 pKa = 11.84 PQPADD27 pKa = 3.44 LGEE30 pKa = 4.02 VSGYY34 pKa = 7.42 EE35 pKa = 3.97 TQLGNNTSDD44 pKa = 3.35 YY45 pKa = 11.23 GNNSDD50 pKa = 6.33 DD51 pKa = 5.35 DD52 pKa = 5.1 DD53 pKa = 5.16 FDD55 pKa = 5.71 NFDD58 pKa = 4.84 DD59 pKa = 6.06 DD60 pKa = 5.95 FDD62 pKa = 5.4 NFDD65 pKa = 5.55 DD66 pKa = 6.39 DD67 pKa = 6.42 FDD69 pKa = 5.44 DD70 pKa = 5.75 FNDD73 pKa = 4.89 DD74 pKa = 5.29 FDD76 pKa = 6.57 DD77 pKa = 4.84 FDD79 pKa = 6.81 DD80 pKa = 5.41 SVAEE84 pKa = 4.13 PPKK87 pKa = 10.49 TRR89 pKa = 11.84 APQNNPINRR98 pKa = 11.84 LSFLPRR104 pKa = 11.84 EE105 pKa = 4.07 VFYY108 pKa = 11.47 NVLDD112 pKa = 3.82 YY113 pKa = 11.31 LPPNDD118 pKa = 4.14 RR119 pKa = 11.84 YY120 pKa = 10.5 EE121 pKa = 4.17 LSEE124 pKa = 4.23 VDD126 pKa = 4.68 DD127 pKa = 4.17 KK128 pKa = 12.13 SNYY131 pKa = 9.74 LSNLHH136 pKa = 6.49 KK137 pKa = 8.47 PTHH140 pKa = 6.51 VILQVTRR147 pKa = 11.84 PWSNYY152 pKa = 7.26 VYY154 pKa = 10.25 SYY156 pKa = 8.91 STSMPIDD163 pKa = 3.17
Molecular weight: 18.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.714
IPC2_protein 3.681
IPC_protein 3.719
Toseland 3.478
ProMoST 3.872
Dawson 3.732
Bjellqvist 3.91
Wikipedia 3.706
Rodwell 3.541
Grimsley 3.376
Solomon 3.719
Lehninger 3.681
Nozaki 3.846
DTASelect 4.164
Thurlkill 3.541
EMBOSS 3.719
Sillero 3.846
Patrickios 1.888
IPC_peptide 3.719
IPC2_peptide 3.808
IPC2.peptide.svr19 3.769
Protein with the highest isoelectric point:
>tr|A0A4Q0A0S3|A0A4Q0A0S3_9FUNG Phosphoinositide phospholipase C (Fragment) OS=Dimargaris cristalligena OX=215637 GN=BJ085DRAFT_16957 PE=4 SV=1
RR1 pKa = 7.89 PYY3 pKa = 9.58 ICAWSTCQKK12 pKa = 10.87 AFARR16 pKa = 11.84 KK17 pKa = 9.03 SDD19 pKa = 3.46 LMRR22 pKa = 11.84 HH23 pKa = 5.04 RR24 pKa = 11.84 RR25 pKa = 11.84 IHH27 pKa = 5.26 TGEE30 pKa = 3.98 RR31 pKa = 11.84 PYY33 pKa = 11.06 PCDD36 pKa = 3.17 WPNCGKK42 pKa = 10.49 RR43 pKa = 11.84 FIQRR47 pKa = 11.84 SALTVHH53 pKa = 5.9 YY54 pKa = 7.38 RR55 pKa = 11.84 THH57 pKa = 5.66 TGEE60 pKa = 4.33 RR61 pKa = 11.84 PHH63 pKa = 6.87 TCDD66 pKa = 3.06 FCNKK70 pKa = 9.29 NFSDD74 pKa = 3.75 SSSLARR80 pKa = 11.84 HH81 pKa = 5.87 RR82 pKa = 11.84 RR83 pKa = 11.84 THH85 pKa = 5.12 TGKK88 pKa = 10.32 RR89 pKa = 11.84 PYY91 pKa = 10.95 ACDD94 pKa = 3.18 HH95 pKa = 6.65 LGCGRR100 pKa = 11.84 TFTRR104 pKa = 11.84 RR105 pKa = 11.84 TTLTKK110 pKa = 10.42 HH111 pKa = 4.64 QQ112 pKa = 3.89
Molecular weight: 13.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.359
IPC2_protein 9.56
IPC_protein 10.306
Toseland 10.467
ProMoST 10.379
Dawson 10.599
Bjellqvist 10.365
Wikipedia 10.804
Rodwell 10.774
Grimsley 10.657
Solomon 10.687
Lehninger 10.657
Nozaki 10.54
DTASelect 10.321
Thurlkill 10.496
EMBOSS 10.862
Sillero 10.555
Patrickios 10.496
IPC_peptide 10.701
IPC2_peptide 9.809
IPC2.peptide.svr19 8.531
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7376
0
7376
2791136
49
7319
378.4
41.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.382 ± 0.029
1.306 ± 0.012
5.455 ± 0.024
5.182 ± 0.03
3.772 ± 0.02
6.115 ± 0.027
2.763 ± 0.017
4.881 ± 0.026
4.002 ± 0.031
9.837 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.13 ± 0.013
3.827 ± 0.019
6.814 ± 0.05
4.49 ± 0.024
5.885 ± 0.024
8.169 ± 0.036
6.566 ± 0.024
6.164 ± 0.031
1.221 ± 0.011
3.04 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here