Hydrogenophaga sp. BA0156

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Hydrogenophaga; unclassified Hydrogenophaga

Average proteome isoelectric point is 7.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4261 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G8IIM5|A0A6G8IIM5_9BURK Uncharacterized protein OS=Hydrogenophaga sp. BA0156 OX=2716225 GN=G9Q37_13040 PE=4 SV=1
MM1 pKa = 7.48SSSPSEE7 pKa = 4.26PDD9 pKa = 3.84SPDD12 pKa = 3.32QPGEE16 pKa = 3.85ASLPCVMSFNANDD29 pKa = 3.73PSGAGGLSADD39 pKa = 3.97LTAMSSASAHH49 pKa = 5.36VLPVVTGTYY58 pKa = 10.45VRR60 pKa = 11.84DD61 pKa = 3.63TTAVRR66 pKa = 11.84DD67 pKa = 4.14HH68 pKa = 6.73IAFDD72 pKa = 3.76EE73 pKa = 4.29EE74 pKa = 4.4AVLDD78 pKa = 3.9QARR81 pKa = 11.84CALEE85 pKa = 4.41DD86 pKa = 3.59MPVQAFKK93 pKa = 11.21VGFVGTPEE101 pKa = 4.06NLSAIAQITSDD112 pKa = 3.77YY113 pKa = 11.41AEE115 pKa = 4.48VPVITYY121 pKa = 9.06MPDD124 pKa = 3.44LSWWDD129 pKa = 3.43EE130 pKa = 4.23LEE132 pKa = 4.17IEE134 pKa = 4.71TYY136 pKa = 11.2LDD138 pKa = 3.44ACTEE142 pKa = 4.14LLLSQTTVLVGNHH155 pKa = 5.0STLSRR160 pKa = 11.84WLLPDD165 pKa = 3.28WEE167 pKa = 4.82GDD169 pKa = 3.48RR170 pKa = 11.84PPGPRR175 pKa = 11.84EE176 pKa = 3.73VARR179 pKa = 11.84AAAVHH184 pKa = 5.67GVSYY188 pKa = 9.71TLVTGFNAADD198 pKa = 3.46QFLEE202 pKa = 4.04SHH204 pKa = 6.76LASPEE209 pKa = 4.24TVHH212 pKa = 6.13ATARR216 pKa = 11.84YY217 pKa = 8.64EE218 pKa = 3.96RR219 pKa = 11.84FEE221 pKa = 4.21ATFSGAGDD229 pKa = 3.74TLSAALCALIAGGADD244 pKa = 3.51LQNACAEE251 pKa = 4.13ALTYY255 pKa = 10.72LDD257 pKa = 3.41QCLDD261 pKa = 3.39AGFQPGMGNAVPDD274 pKa = 3.88RR275 pKa = 11.84LFWAHH280 pKa = 7.54DD281 pKa = 3.82DD282 pKa = 3.94DD283 pKa = 4.96EE284 pKa = 5.21EE285 pKa = 6.0PGGAPAAEE293 pKa = 4.28PASDD297 pKa = 3.71STLDD301 pKa = 4.15ADD303 pKa = 5.84DD304 pKa = 5.23FPLDD308 pKa = 3.9TTRR311 pKa = 11.84HH312 pKa = 4.6

Molecular weight: 33.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G8ILB7|A0A6G8ILB7_9BURK 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase OS=Hydrogenophaga sp. BA0156 OX=2716225 GN=hpaE PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.07QPSKK9 pKa = 9.07IRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.91GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4261

0

4261

1407824

28

4226

330.4

35.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.627 ± 0.065

0.892 ± 0.012

5.11 ± 0.025

5.248 ± 0.034

3.364 ± 0.022

8.47 ± 0.036

2.397 ± 0.02

3.865 ± 0.028

2.808 ± 0.029

11.223 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.291 ± 0.02

2.396 ± 0.021

5.591 ± 0.03

4.09 ± 0.026

7.551 ± 0.035

4.861 ± 0.026

4.936 ± 0.022

7.721 ± 0.03

1.581 ± 0.019

1.979 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski