Pseudomonas virus PaP3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Bruynoghevirus

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q774I3|Q774I3_9CAUD ORF.71 OS=Pseudomonas virus PaP3 OX=188350 GN=orf71 PE=4 SV=1
MM1 pKa = 7.86IEE3 pKa = 4.27GEE5 pKa = 4.33LVGHH9 pKa = 5.21VTLFLVLEE17 pKa = 4.49PGFLCGTLQTSGLVLGEE34 pKa = 4.49DD35 pKa = 3.41EE36 pKa = 6.15RR37 pKa = 11.84EE38 pKa = 3.94FLGLFLVGIGLDD50 pKa = 3.36LAVVYY55 pKa = 10.49EE56 pKa = 4.38PMGQGQPAGALRR68 pKa = 11.84HH69 pKa = 5.83RR70 pKa = 11.84VEE72 pKa = 3.86QLAGQGHH79 pKa = 7.31LAMLVDD85 pKa = 4.17VLEE88 pKa = 5.57DD89 pKa = 3.13IHH91 pKa = 7.36TSGLNQLVQGSTPVGNGLDD110 pKa = 3.99DD111 pKa = 4.39PVDD114 pKa = 3.72ALVVAHH120 pKa = 6.82CNYY123 pKa = 10.24LSCLLRR129 pKa = 11.84QQ130 pKa = 3.63

Molecular weight: 13.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8H9Y3|Q8H9Y3_9CAUD ORF.69 OS=Pseudomonas virus PaP3 OX=188350 GN=orf69 PE=4 SV=1
MM1 pKa = 7.57KK2 pKa = 10.34SPYY5 pKa = 9.36EE6 pKa = 4.1AAHH9 pKa = 6.38EE10 pKa = 3.86RR11 pKa = 11.84AQMINRR17 pKa = 11.84LKK19 pKa = 10.78KK20 pKa = 7.41LTRR23 pKa = 11.84MIRR26 pKa = 11.84VHH28 pKa = 7.21PDD30 pKa = 2.8PRR32 pKa = 11.84WIVEE36 pKa = 3.95RR37 pKa = 11.84QEE39 pKa = 4.88LIRR42 pKa = 11.84KK43 pKa = 8.89LSKK46 pKa = 10.75

Molecular weight: 5.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

14108

26

1056

195.9

21.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.477 ± 0.595

1.049 ± 0.161

6.124 ± 0.224

7.081 ± 0.415

3.906 ± 0.242

8.364 ± 0.367

2.048 ± 0.197

5.153 ± 0.209

5.451 ± 0.326

8.541 ± 0.273

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.807 ± 0.179

4.324 ± 0.252

4.189 ± 0.209

3.884 ± 0.268

5.805 ± 0.27

5.642 ± 0.242

5.082 ± 0.244

7.102 ± 0.284

1.623 ± 0.148

3.346 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski