Halovirus HRTV-7
Average proteome isoelectric point is 4.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 105 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R4TFA6|R4TFA6_9CAUD Uncharacterized protein OS=Halovirus HRTV-7 OX=1273754 GN=9 PE=4 SV=1
MM1 pKa = 7.43 TKK3 pKa = 10.58 DD4 pKa = 3.51 DD5 pKa = 3.91 TPEE8 pKa = 4.46 PEE10 pKa = 3.77 TRR12 pKa = 11.84 LTVEE16 pKa = 4.32 DD17 pKa = 4.76 GFLEE21 pKa = 5.47 GYY23 pKa = 9.11 LHH25 pKa = 7.11 GMLAGYY31 pKa = 9.3 YY32 pKa = 9.74 GHH34 pKa = 7.36 EE35 pKa = 5.06 DD36 pKa = 3.64 DD37 pKa = 5.68 GAPAPACEE45 pKa = 4.72 DD46 pKa = 3.76 CSCSIEE52 pKa = 4.42 DD53 pKa = 3.83 SMGRR57 pKa = 11.84 EE58 pKa = 3.99 PDD60 pKa = 3.77 LEE62 pKa = 4.54 TVSAPAALDD71 pKa = 3.46 FQEE74 pKa = 4.05 QVRR77 pKa = 11.84 DD78 pKa = 3.4 YY79 pKa = 11.22 LLRR82 pKa = 11.84 YY83 pKa = 9.85 DD84 pKa = 6.17 LIDD87 pKa = 3.92 EE88 pKa = 5.43 DD89 pKa = 5.34 DD90 pKa = 4.32 LVSQVVVCPEE100 pKa = 3.68 EE101 pKa = 4.02 VRR103 pKa = 11.84 ALVEE107 pKa = 3.95 RR108 pKa = 11.84 AA109 pKa = 3.18
Molecular weight: 12.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.718
IPC2_protein 3.821
IPC_protein 3.795
Toseland 3.592
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.694
Rodwell 3.617
Grimsley 3.503
Solomon 3.757
Lehninger 3.719
Nozaki 3.897
DTASelect 4.088
Thurlkill 3.643
EMBOSS 3.706
Sillero 3.91
Patrickios 0.985
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.805
Protein with the highest isoelectric point:
>tr|R4T5R3|R4T5R3_9CAUD Uncharacterized protein OS=Halovirus HRTV-7 OX=1273754 GN=93 PE=4 SV=1
MM1 pKa = 7.71 KK2 pKa = 10.14 FPKK5 pKa = 9.9 PSVRR9 pKa = 11.84 MVEE12 pKa = 3.87 RR13 pKa = 11.84 TPRR16 pKa = 11.84 YY17 pKa = 9.81 SPGFKK22 pKa = 9.64 YY23 pKa = 10.38 RR24 pKa = 11.84 SIITPRR30 pKa = 11.84 GIYY33 pKa = 9.81 RR34 pKa = 11.84 SLTVRR39 pKa = 11.84 LPVVNRR45 pKa = 11.84 SVILGLKK52 pKa = 9.59 PDD54 pKa = 4.82 DD55 pKa = 3.83 PAEE58 pKa = 4.58 FYY60 pKa = 10.61 TEE62 pKa = 4.19 SQQGEE67 pKa = 4.52 SLL69 pKa = 3.59
Molecular weight: 7.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.274
IPC2_protein 9.721
IPC_protein 10.365
Toseland 10.277
ProMoST 10.043
Dawson 10.482
Bjellqvist 10.189
Wikipedia 10.687
Rodwell 10.73
Grimsley 10.57
Solomon 10.54
Lehninger 10.496
Nozaki 10.233
DTASelect 10.189
Thurlkill 10.321
EMBOSS 10.672
Sillero 10.394
Patrickios 10.452
IPC_peptide 10.54
IPC2_peptide 8.975
IPC2.peptide.svr19 8.656
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
105
0
105
20523
33
946
195.5
21.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.986 ± 0.256
0.755 ± 0.097
8.878 ± 0.291
9.901 ± 0.323
3.606 ± 0.149
7.757 ± 0.356
2.31 ± 0.135
4.566 ± 0.188
3.684 ± 0.229
6.9 ± 0.222
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.417 ± 0.138
3.913 ± 0.165
4.838 ± 0.138
3.07 ± 0.131
6.256 ± 0.233
5.759 ± 0.21
5.701 ± 0.21
6.939 ± 0.185
1.433 ± 0.113
3.333 ± 0.135
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here