Alternaria alternata fusarivirus 1
Average proteome isoelectric point is 7.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z6DR81|A0A2Z6DR81_9VIRU Uncharacterized protein OS=Alternaria alternata fusarivirus 1 OX=1935440 PE=4 SV=1
MM1 pKa = 7.48 KK2 pKa = 10.26 LVLLIRR8 pKa = 11.84 PNPEE12 pKa = 3.22 EE13 pKa = 4.08 EE14 pKa = 4.15 EE15 pKa = 4.3 AVGSRR20 pKa = 11.84 WCWKK24 pKa = 9.45 HH25 pKa = 5.31 PPYY28 pKa = 10.8 VLSLSGTGFMSVRR41 pKa = 11.84 GGGRR45 pKa = 11.84 CAVRR49 pKa = 11.84 AEE51 pKa = 4.09 MFGNPEE57 pKa = 4.23 CNDD60 pKa = 3.46 CEE62 pKa = 4.51 LFTRR66 pKa = 11.84 FSS68 pKa = 3.37
Molecular weight: 7.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.13
IPC2_protein 6.16
IPC_protein 6.351
Toseland 6.173
ProMoST 6.478
Dawson 6.795
Bjellqvist 6.547
Wikipedia 6.751
Rodwell 6.781
Grimsley 6.173
Solomon 6.854
Lehninger 6.868
Nozaki 6.912
DTASelect 7.044
Thurlkill 7.073
EMBOSS 7.132
Sillero 7.19
Patrickios 4.101
IPC_peptide 6.868
IPC2_peptide 6.985
IPC2.peptide.svr19 6.893
Protein with the highest isoelectric point:
>tr|A0A2Z6DR81|A0A2Z6DR81_9VIRU Uncharacterized protein OS=Alternaria alternata fusarivirus 1 OX=1935440 PE=4 SV=1
MM1 pKa = 7.21 TSEE4 pKa = 4.37 KK5 pKa = 10.75 VFIGKK10 pKa = 10.04 RR11 pKa = 11.84 IVQTRR16 pKa = 11.84 EE17 pKa = 3.3 QLSQVEE23 pKa = 4.1 LTAQQVKK30 pKa = 10.47 SSTVWLVRR38 pKa = 11.84 EE39 pKa = 4.29 PGWVSGQEE47 pKa = 4.03 KK48 pKa = 10.43 LNGWFLEE55 pKa = 4.26 VEE57 pKa = 4.09 GTDD60 pKa = 3.0 IVLCVTTLFKK70 pKa = 11.58 GEE72 pKa = 4.18 VTSEE76 pKa = 3.5 RR77 pKa = 11.84 WFPPSPVFKK86 pKa = 10.0 WKK88 pKa = 10.39 KK89 pKa = 9.02 EE90 pKa = 3.85 YY91 pKa = 10.14 QSRR94 pKa = 11.84 DD95 pKa = 3.15 KK96 pKa = 10.94 EE97 pKa = 4.55 VPTEE101 pKa = 3.81 VAYY104 pKa = 10.2 KK105 pKa = 9.68 NVIVARR111 pKa = 11.84 MADD114 pKa = 3.87 EE115 pKa = 4.52 NLLGYY120 pKa = 10.93 YY121 pKa = 10.95 DD122 pKa = 4.64 MLTPATPLVNRR133 pKa = 11.84 LSASFKK139 pKa = 8.0 WWKK142 pKa = 8.96 TGGNAPIIPFSPTNSKK158 pKa = 10.78 KK159 pKa = 10.64 EE160 pKa = 3.72 IIGRR164 pKa = 11.84 ISHH167 pKa = 7.03 FEE169 pKa = 3.76 TSFAALVNPPEE180 pKa = 4.12 FALDD184 pKa = 3.3 GLLALRR190 pKa = 11.84 VLKK193 pKa = 10.71 DD194 pKa = 3.4 QLPSMKK200 pKa = 10.31 DD201 pKa = 2.56 RR202 pKa = 11.84 GTQNVSRR209 pKa = 11.84 FLTTLVKK216 pKa = 10.09 AWAMSKK222 pKa = 10.14 QPSHH226 pKa = 7.01 FKK228 pKa = 11.08 GPLSKK233 pKa = 9.93 ISYY236 pKa = 8.93 PVEE239 pKa = 4.42 KK240 pKa = 10.46 DD241 pKa = 3.14 GLPADD246 pKa = 4.22 RR247 pKa = 11.84 AKK249 pKa = 10.07 MYY251 pKa = 11.0 HH252 pKa = 6.41 NMICGKK258 pKa = 10.06 GDD260 pKa = 3.84 EE261 pKa = 4.36 ITRR264 pKa = 11.84 GLGFCMWTPPRR275 pKa = 11.84 ITVTTDD281 pKa = 2.66 AEE283 pKa = 4.69 GFTTKK288 pKa = 9.8 SRR290 pKa = 11.84 KK291 pKa = 8.54 KK292 pKa = 9.84 HH293 pKa = 4.62 EE294 pKa = 4.46 EE295 pKa = 3.73 KK296 pKa = 10.72 KK297 pKa = 10.8 IMLDD301 pKa = 3.97 LGPDD305 pKa = 3.41 IQVGGPNEE313 pKa = 3.8 TRR315 pKa = 11.84 LRR317 pKa = 11.84 EE318 pKa = 3.92 EE319 pKa = 4.41 SEE321 pKa = 4.45 AEE323 pKa = 4.06 SSAMGARR330 pKa = 11.84 RR331 pKa = 11.84 QHH333 pKa = 5.91 AKK335 pKa = 10.53 KK336 pKa = 10.62 NSDD339 pKa = 3.47 PDD341 pKa = 3.54 NGGFNPTSKK350 pKa = 10.35 VSQKK354 pKa = 11.03 DD355 pKa = 3.5 QVSEE359 pKa = 4.22 DD360 pKa = 3.88 AVDD363 pKa = 5.64 DD364 pKa = 3.94 ATDD367 pKa = 3.19 WLRR370 pKa = 11.84 NSFTMTNFRR379 pKa = 11.84 RR380 pKa = 11.84 FYY382 pKa = 10.38 RR383 pKa = 11.84 RR384 pKa = 11.84 VKK386 pKa = 10.49 RR387 pKa = 11.84 SVSSIKK393 pKa = 10.69 DD394 pKa = 3.55 SVTHH398 pKa = 6.19 EE399 pKa = 4.08 GVGIKK404 pKa = 10.38 KK405 pKa = 10.07 PFTEE409 pKa = 4.14 KK410 pKa = 10.95 AKK412 pKa = 10.48 DD413 pKa = 3.4 AVVFTVTSAVKK424 pKa = 10.34 ACATVPFAGAMGLWYY439 pKa = 10.12 GIKK442 pKa = 10.36 EE443 pKa = 4.04 PFVSIKK449 pKa = 10.2 EE450 pKa = 4.05 FPFPEE455 pKa = 4.49 GRR457 pKa = 11.84 LEE459 pKa = 4.24 VAKK462 pKa = 10.58 HH463 pKa = 4.96 VADD466 pKa = 3.77 CAVYY470 pKa = 10.1 SLSSVVTSTISGVFAGLASATRR492 pKa = 3.68
Molecular weight: 54.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.8
IPC2_protein 8.565
IPC_protein 8.448
Toseland 9.414
ProMoST 9.121
Dawson 9.619
Bjellqvist 9.311
Wikipedia 9.736
Rodwell 10.087
Grimsley 9.648
Solomon 9.663
Lehninger 9.633
Nozaki 9.545
DTASelect 9.253
Thurlkill 9.502
EMBOSS 9.838
Sillero 9.575
Patrickios 5.944
IPC_peptide 9.663
IPC2_peptide 7.79
IPC2.peptide.svr19 7.865
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
2082
68
1522
694.0
78.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.676 ± 0.289
0.768 ± 0.715
4.947 ± 0.515
6.052 ± 0.735
5.235 ± 0.089
5.956 ± 0.781
1.873 ± 0.247
4.947 ± 0.736
6.532 ± 0.919
9.99 ± 1.786
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.738 ± 0.21
3.65 ± 0.275
4.803 ± 0.641
2.546 ± 0.304
6.196 ± 0.382
7.589 ± 0.357
6.724 ± 0.495
7.541 ± 0.584
2.45 ± 0.1
2.786 ± 0.56
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here