Capybara microvirus Cap3_SP_444
Average proteome isoelectric point is 5.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W514|A0A4P8W514_9VIRU Minor capsid protein OS=Capybara microvirus Cap3_SP_444 OX=2585456 PE=4 SV=1
MM1 pKa = 7.68 AKK3 pKa = 10.36 FKK5 pKa = 9.75 TAYY8 pKa = 10.74 SNDD11 pKa = 3.32 HH12 pKa = 5.58 VVYY15 pKa = 10.45 SQDD18 pKa = 3.15 LSRR21 pKa = 11.84 VEE23 pKa = 4.08 EE24 pKa = 4.77 PILAEE29 pKa = 4.1 VVEE32 pKa = 4.59 LEE34 pKa = 4.3 FNEE37 pKa = 4.52 GLDD40 pKa = 4.38 DD41 pKa = 3.88 SGNPIRR47 pKa = 11.84 IAKK50 pKa = 8.01 EE51 pKa = 3.34 HH52 pKa = 6.17 LVIKK56 pKa = 10.16 EE57 pKa = 3.49 IGKK60 pKa = 10.23 RR61 pKa = 11.84 NIQEE65 pKa = 4.45 EE66 pKa = 4.54 INASAIGCSVYY77 pKa = 10.65 EE78 pKa = 3.79 ILARR82 pKa = 11.84 TNGEE86 pKa = 3.97 VPTANGTFGNIADD99 pKa = 4.1 SPKK102 pKa = 10.93 DD103 pKa = 3.31 LLEE106 pKa = 4.16 AVEE109 pKa = 4.23 LNKK112 pKa = 10.91 NIDD115 pKa = 3.5 QALDD119 pKa = 3.87 DD120 pKa = 4.06 YY121 pKa = 11.25 KK122 pKa = 11.13 GAYY125 pKa = 9.58 SSIEE129 pKa = 3.79 EE130 pKa = 4.32 LLNDD134 pKa = 3.78 VSNKK138 pKa = 9.72 KK139 pKa = 9.64 LQAYY143 pKa = 10.12 LDD145 pKa = 3.85 AKK147 pKa = 10.59 LAEE150 pKa = 4.55 LKK152 pKa = 10.87 VKK154 pKa = 10.65 EE155 pKa = 4.42 GDD157 pKa = 3.5 SHH159 pKa = 6.13 EE160 pKa = 4.16
Molecular weight: 17.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.611
IPC2_protein 4.571
IPC_protein 4.482
Toseland 4.329
ProMoST 4.533
Dawson 4.418
Bjellqvist 4.596
Wikipedia 4.279
Rodwell 4.317
Grimsley 4.24
Solomon 4.418
Lehninger 4.368
Nozaki 4.52
DTASelect 4.66
Thurlkill 4.329
EMBOSS 4.291
Sillero 4.584
Patrickios 3.923
IPC_peptide 4.418
IPC2_peptide 4.584
IPC2.peptide.svr19 4.511
Protein with the highest isoelectric point:
>tr|A0A4V1FVT5|A0A4V1FVT5_9VIRU Replication initiator protein OS=Capybara microvirus Cap3_SP_444 OX=2585456 PE=4 SV=1
MM1 pKa = 7.69 CLYY4 pKa = 9.66 PLNGFWTGTYY14 pKa = 9.47 TDD16 pKa = 3.75 NGKK19 pKa = 10.19 KK20 pKa = 10.48 DD21 pKa = 4.19 FIVVDD26 pKa = 4.8 GKK28 pKa = 9.4 TKK30 pKa = 10.58 LLSFTRR36 pKa = 11.84 FQKK39 pKa = 10.58 LYY41 pKa = 10.48 KK42 pKa = 9.73 KK43 pKa = 9.8 AHH45 pKa = 6.61 KK46 pKa = 9.88 YY47 pKa = 10.13 DD48 pKa = 3.36 LRR50 pKa = 11.84 QFHH53 pKa = 6.02 QFDD56 pKa = 3.97 GEE58 pKa = 4.53 LYY60 pKa = 9.39 LTDD63 pKa = 4.55 PVAIPCGRR71 pKa = 11.84 CIEE74 pKa = 4.35 CRR76 pKa = 11.84 LSYY79 pKa = 10.94 SRR81 pKa = 11.84 DD82 pKa = 2.89 WALRR86 pKa = 11.84 CVLEE90 pKa = 4.49 SQDD93 pKa = 4.21 HH94 pKa = 5.5 EE95 pKa = 4.63 FNYY98 pKa = 10.36 FLTLTYY104 pKa = 10.98 DD105 pKa = 3.48 NSYY108 pKa = 11.2 LPANGSLVKK117 pKa = 10.34 KK118 pKa = 10.32 DD119 pKa = 3.14 VQLFIKK125 pKa = 10.48 RR126 pKa = 11.84 LRR128 pKa = 11.84 KK129 pKa = 10.33 YY130 pKa = 9.75 IDD132 pKa = 3.32 SHH134 pKa = 7.18 VGSYY138 pKa = 9.8 NIKK141 pKa = 9.79 YY142 pKa = 8.04 FLCGEE147 pKa = 4.32 YY148 pKa = 10.6 GEE150 pKa = 5.11 HH151 pKa = 5.59 SHH153 pKa = 6.82 RR154 pKa = 11.84 PHH156 pKa = 5.82 YY157 pKa = 10.43 HH158 pKa = 6.86 LLLFNCPLPCLVPLHH173 pKa = 7.2 DD174 pKa = 4.38 NLFVSEE180 pKa = 4.8 PLFKK184 pKa = 10.56 LWPFGSHH191 pKa = 6.71 VIGDD195 pKa = 3.72 VTFEE199 pKa = 3.94 SAAYY203 pKa = 7.85 VARR206 pKa = 11.84 YY207 pKa = 9.08 VMKK210 pKa = 10.22 KK211 pKa = 9.81 CNSSSLVFDD220 pKa = 4.1 SSVEE224 pKa = 3.93 PEE226 pKa = 4.12 FVLMSRR232 pKa = 11.84 RR233 pKa = 11.84 PGIGKK238 pKa = 9.81 NYY240 pKa = 9.74 FDD242 pKa = 3.41 SHH244 pKa = 6.67 YY245 pKa = 11.43 DD246 pKa = 3.29 RR247 pKa = 11.84 IYY249 pKa = 11.1 KK250 pKa = 10.07 LDD252 pKa = 3.99 SIYY255 pKa = 10.54 LARR258 pKa = 11.84 YY259 pKa = 7.61 GIPLRR264 pKa = 11.84 FKK266 pKa = 9.81 PPRR269 pKa = 11.84 YY270 pKa = 9.2 FDD272 pKa = 3.58 RR273 pKa = 11.84 LYY275 pKa = 11.23 EE276 pKa = 4.8 KK277 pKa = 10.13 IDD279 pKa = 3.45 SDD281 pKa = 3.89 FLSSVKK287 pKa = 10.57 AEE289 pKa = 3.66 RR290 pKa = 11.84 LAFSKK295 pKa = 10.76 SSSRR299 pKa = 11.84 ASFLHH304 pKa = 7.03 IGEE307 pKa = 5.19 TIDD310 pKa = 4.38 SYY312 pKa = 11.79 DD313 pKa = 3.14 ISLVNHH319 pKa = 5.84 HH320 pKa = 6.41 VEE322 pKa = 3.86 YY323 pKa = 10.95 LKK325 pKa = 8.84 THH327 pKa = 5.83 RR328 pKa = 11.84 LKK330 pKa = 11.31 LLVRR334 pKa = 11.84 TLL336 pKa = 3.39
Molecular weight: 39.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.124
IPC2_protein 8.185
IPC_protein 8.039
Toseland 8.024
ProMoST 8.521
Dawson 8.77
Bjellqvist 8.931
Wikipedia 8.799
Rodwell 8.814
Grimsley 8.068
Solomon 8.843
Lehninger 8.829
Nozaki 8.99
DTASelect 8.741
Thurlkill 8.785
EMBOSS 8.916
Sillero 9.033
Patrickios 3.884
IPC_peptide 8.843
IPC2_peptide 7.849
IPC2.peptide.svr19 7.95
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1378
87
541
229.7
25.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.096 ± 1.719
1.451 ± 0.555
6.313 ± 0.288
5.152 ± 1.129
5.37 ± 0.84
4.935 ± 0.584
2.104 ± 0.753
6.967 ± 1.631
6.241 ± 0.971
9.289 ± 1.424
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.104 ± 0.592
5.878 ± 0.859
4.282 ± 0.86
2.903 ± 0.631
4.427 ± 0.715
9.289 ± 0.633
6.023 ± 1.181
5.443 ± 0.398
0.726 ± 0.295
5.007 ± 0.907
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here