Drosophila busckii (Fruit fly)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha; Schizophora; Acalyptrat

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11914 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M4EM95|A0A0M4EM95_DROBS CG8500 OS=Drosophila busckii OX=30019 GN=Dbus_chr3Rg1290 PE=4 SV=1
MM1 pKa = 7.87ANLQADD7 pKa = 4.12KK8 pKa = 11.63SNGNCVYY15 pKa = 11.01ADD17 pKa = 4.02SCCHH21 pKa = 6.54TDD23 pKa = 3.22NAVATTATATSTTQNTTNTINIMTGAAAAAAVAASLPTSASCEE66 pKa = 4.08DD67 pKa = 4.08LDD69 pKa = 5.51LDD71 pKa = 4.12HH72 pKa = 6.77SQSLSEE78 pKa = 4.1YY79 pKa = 8.64TDD81 pKa = 3.56ADD83 pKa = 3.78EE84 pKa = 5.73SISAPTEE91 pKa = 3.66FLAEE95 pKa = 3.95FLSAVMLKK103 pKa = 10.31DD104 pKa = 3.34YY105 pKa = 11.09KK106 pKa = 10.76KK107 pKa = 10.48ALKK110 pKa = 9.48YY111 pKa = 10.51CKK113 pKa = 10.19LILQYY118 pKa = 10.82EE119 pKa = 4.47PNNTTAKK126 pKa = 10.05EE127 pKa = 4.1FYY129 pKa = 10.33PLILDD134 pKa = 3.91KK135 pKa = 11.29LRR137 pKa = 11.84VAAASDD143 pKa = 3.5SDD145 pKa = 3.65EE146 pKa = 4.72NYY148 pKa = 10.5NKK150 pKa = 10.42SSSPEE155 pKa = 3.82LNLEE159 pKa = 4.35LEE161 pKa = 4.56LEE163 pKa = 4.25AAADD167 pKa = 3.92CEE169 pKa = 4.22EE170 pKa = 4.85EE171 pKa = 4.1EE172 pKa = 5.57DD173 pKa = 4.16SVSSNSSDD181 pKa = 3.46ADD183 pKa = 3.52NDD185 pKa = 4.18DD186 pKa = 4.05EE187 pKa = 5.55VDD189 pKa = 4.23AEE191 pKa = 4.37PEE193 pKa = 3.98DD194 pKa = 4.33EE195 pKa = 4.64EE196 pKa = 4.68EE197 pKa = 4.38SSSGDD202 pKa = 3.82DD203 pKa = 3.44YY204 pKa = 11.54EE205 pKa = 5.59LPIDD209 pKa = 4.16EE210 pKa = 5.13PQDD213 pKa = 3.56VAAVGSLAAHH223 pKa = 5.51SHH225 pKa = 5.74SLSHH229 pKa = 6.63HH230 pKa = 6.11NSGSSRR236 pKa = 11.84NSSSGSGGRR245 pKa = 11.84SDD247 pKa = 3.93NTTHH251 pKa = 6.84SYY253 pKa = 11.27SSLLLEE259 pKa = 5.32DD260 pKa = 5.51DD261 pKa = 3.97NDD263 pKa = 3.79IVPLNLQFGNKK274 pKa = 8.87DD275 pKa = 3.18CHH277 pKa = 6.95AADD280 pKa = 4.37LDD282 pKa = 3.96VSNGNKK288 pKa = 9.39LASASCSDD296 pKa = 3.94SEE298 pKa = 5.32SPTEE302 pKa = 3.98PLTQRR307 pKa = 11.84LAAILRR313 pKa = 11.84SKK315 pKa = 10.79CGVSASNDD323 pKa = 3.23DD324 pKa = 3.77NYY326 pKa = 11.98

Molecular weight: 34.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M4F6C3|A0A0M4F6C3_DROBS Cyp6d5 OS=Drosophila busckii OX=30019 GN=Dbus_chr3Rg1982 PE=3 SV=1
VV1 pKa = 4.81QTGISLNDD9 pKa = 4.16RR10 pKa = 11.84FTQMQSRR17 pKa = 11.84QQQTAEE23 pKa = 3.82TRR25 pKa = 11.84GRR27 pKa = 11.84RR28 pKa = 11.84SRR30 pKa = 11.84SRR32 pKa = 11.84SRR34 pKa = 11.84SRR36 pKa = 11.84RR37 pKa = 11.84IVDD40 pKa = 3.51SAPSVANTRR49 pKa = 11.84LLQEE53 pKa = 5.16FKK55 pKa = 10.51RR56 pKa = 11.84KK57 pKa = 8.51HH58 pKa = 4.68TVQTALKK65 pKa = 9.27LKK67 pKa = 10.04RR68 pKa = 11.84RR69 pKa = 11.84SLRR72 pKa = 11.84TSASGASGMKK82 pKa = 10.23ALRR85 pKa = 11.84LGPNGKK91 pKa = 8.57PVRR94 pKa = 11.84SSNISRR100 pKa = 11.84LATMRR105 pKa = 11.84ADD107 pKa = 3.83LVASNGRR114 pKa = 11.84ARR116 pKa = 11.84RR117 pKa = 11.84SGSTTRR123 pKa = 11.84RR124 pKa = 11.84SNSAVRR130 pKa = 11.84GLRR133 pKa = 11.84QRR135 pKa = 11.84LGNRR139 pKa = 11.84RR140 pKa = 11.84PTVGGAAEE148 pKa = 3.98RR149 pKa = 11.84VAQRR153 pKa = 11.84RR154 pKa = 11.84RR155 pKa = 11.84GSLGRR160 pKa = 11.84FQQQQQQQQVLAPARR175 pKa = 11.84SRR177 pKa = 11.84SRR179 pKa = 11.84SRR181 pKa = 11.84ARR183 pKa = 11.84NTLSQPRR190 pKa = 11.84GRR192 pKa = 11.84ALSQPRR198 pKa = 11.84GRR200 pKa = 11.84PNSQVRR206 pKa = 11.84GRR208 pKa = 11.84EE209 pKa = 3.68RR210 pKa = 11.84SLSRR214 pKa = 11.84GRR216 pKa = 11.84DD217 pKa = 2.95RR218 pKa = 11.84SMGRR222 pKa = 11.84MPEE225 pKa = 3.86RR226 pKa = 11.84SQPRR230 pKa = 11.84ARR232 pKa = 11.84SQSRR236 pKa = 11.84GRR238 pKa = 11.84PPQVANSRR246 pKa = 11.84VSVKK250 pKa = 9.86QRR252 pKa = 11.84LGVRR256 pKa = 11.84PGVAATVAAAKK267 pKa = 9.85KK268 pKa = 9.88RR269 pKa = 11.84RR270 pKa = 11.84GNRR273 pKa = 11.84RR274 pKa = 11.84NSGQLPGVAAGRR286 pKa = 11.84IEE288 pKa = 3.81RR289 pKa = 11.84RR290 pKa = 11.84RR291 pKa = 11.84NSNVAQKK298 pKa = 10.77AVATAAGGRR307 pKa = 11.84GRR309 pKa = 11.84KK310 pKa = 8.93GRR312 pKa = 11.84ARR314 pKa = 11.84GRR316 pKa = 11.84SAVRR320 pKa = 11.84AAANASAAAVATQRR334 pKa = 11.84SRR336 pKa = 11.84SRR338 pKa = 11.84SRR340 pKa = 11.84SRR342 pKa = 11.84SRR344 pKa = 11.84ARR346 pKa = 11.84QGNAAAAGGAAAGKK360 pKa = 10.19GAGQSRR366 pKa = 11.84GRR368 pKa = 11.84PRR370 pKa = 11.84GRR372 pKa = 11.84AGKK375 pKa = 9.04VVKK378 pKa = 9.31ATNKK382 pKa = 10.21NGNNKK387 pKa = 9.63NSNINKK393 pKa = 9.47NKK395 pKa = 8.32NTGAGKK401 pKa = 10.35ANQQQGRR408 pKa = 11.84RR409 pKa = 11.84GRR411 pKa = 11.84SRR413 pKa = 11.84VRR415 pKa = 11.84GGGAGKK421 pKa = 8.51PQRR424 pKa = 11.84KK425 pKa = 6.99EE426 pKa = 3.91VKK428 pKa = 10.35RR429 pKa = 11.84EE430 pKa = 3.96DD431 pKa = 3.57LDD433 pKa = 4.86KK434 pKa = 11.45EE435 pKa = 4.02LDD437 pKa = 3.51QYY439 pKa = 11.55MSTTKK444 pKa = 11.22GEE446 pKa = 3.74MDD448 pKa = 4.0YY449 pKa = 11.4LLKK452 pKa = 10.93

Molecular weight: 49.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11914

0

11914

6422999

8

18943

539.1

60.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.149 ± 0.029

1.976 ± 0.019

5.053 ± 0.02

6.312 ± 0.033

3.508 ± 0.015

5.454 ± 0.026

2.666 ± 0.012

4.87 ± 0.019

5.493 ± 0.025

9.665 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.37 ± 0.01

4.861 ± 0.016

5.094 ± 0.024

5.644 ± 0.032

5.426 ± 0.021

7.91 ± 0.032

5.708 ± 0.022

5.681 ± 0.019

1.018 ± 0.007

3.141 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski