Amino acid dipepetide frequency for Lactococcus phage r1t (Bacteriophage r1t)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.359AlaAla: 3.359 ± 0.776
0.296AlaCys: 0.296 ± 0.169
4.248AlaAsp: 4.248 ± 0.739
4.94AlaGlu: 4.94 ± 0.81
2.667AlaPhe: 2.667 ± 0.506
4.742AlaGly: 4.742 ± 0.96
0.988AlaHis: 0.988 ± 0.334
5.631AlaIle: 5.631 ± 0.868
4.94AlaLys: 4.94 ± 0.67
5.73AlaLeu: 5.73 ± 0.887
1.877AlaMet: 1.877 ± 0.541
3.655AlaAsn: 3.655 ± 0.699
0.988AlaPro: 0.988 ± 0.349
2.865AlaGln: 2.865 ± 0.55
2.47AlaArg: 2.47 ± 0.587
4.149AlaSer: 4.149 ± 0.713
4.94AlaThr: 4.94 ± 0.631
4.149AlaVal: 4.149 ± 0.622
0.692AlaTrp: 0.692 ± 0.231
1.877AlaTyr: 1.877 ± 0.437
0.0AlaXaa: 0.0 ± 0.0
Cys
0.198CysAla: 0.198 ± 0.142
0.099CysCys: 0.099 ± 0.098
0.494CysAsp: 0.494 ± 0.257
0.0CysGlu: 0.0 ± 0.0
0.099CysPhe: 0.099 ± 0.105
0.79CysGly: 0.79 ± 0.349
0.296CysHis: 0.296 ± 0.172
0.099CysIle: 0.099 ± 0.1
0.099CysLys: 0.099 ± 0.098
0.494CysLeu: 0.494 ± 0.251
0.0CysMet: 0.0 ± 0.0
0.395CysAsn: 0.395 ± 0.217
0.0CysPro: 0.0 ± 0.0
0.395CysGln: 0.395 ± 0.181
0.198CysArg: 0.198 ± 0.182
0.296CysSer: 0.296 ± 0.154
0.198CysThr: 0.198 ± 0.125
0.0CysVal: 0.0 ± 0.0
0.0CysTrp: 0.0 ± 0.0
0.296CysTyr: 0.296 ± 0.157
0.0CysXaa: 0.0 ± 0.0
Asp
3.359AspAla: 3.359 ± 0.578
0.593AspCys: 0.593 ± 0.25
3.952AspAsp: 3.952 ± 0.631
5.236AspGlu: 5.236 ± 0.835
3.359AspPhe: 3.359 ± 0.497
3.754AspGly: 3.754 ± 0.572
1.087AspHis: 1.087 ± 0.304
4.545AspIle: 4.545 ± 0.784
5.533AspLys: 5.533 ± 0.793
4.545AspLeu: 4.545 ± 0.792
2.272AspMet: 2.272 ± 0.501
4.643AspAsn: 4.643 ± 0.643
1.778AspPro: 1.778 ± 0.533
0.988AspGln: 0.988 ± 0.307
2.766AspArg: 2.766 ± 0.634
4.149AspSer: 4.149 ± 0.616
3.063AspThr: 3.063 ± 0.606
3.557AspVal: 3.557 ± 0.661
1.186AspTrp: 1.186 ± 0.349
2.865AspTyr: 2.865 ± 0.649
0.0AspXaa: 0.0 ± 0.0
Glu
5.137GluAla: 5.137 ± 0.665
0.395GluCys: 0.395 ± 0.188
3.161GluAsp: 3.161 ± 0.73
4.841GluGlu: 4.841 ± 1.079
3.063GluPhe: 3.063 ± 0.637
2.272GluGly: 2.272 ± 0.443
1.482GluHis: 1.482 ± 0.411
5.533GluIle: 5.533 ± 0.574
6.323GluLys: 6.323 ± 1.062
6.718GluLeu: 6.718 ± 0.861
2.47GluMet: 2.47 ± 0.36
4.149GluAsn: 4.149 ± 0.674
2.272GluPro: 2.272 ± 0.55
4.545GluGln: 4.545 ± 0.704
3.161GluArg: 3.161 ± 0.732
4.347GluSer: 4.347 ± 0.735
3.952GluThr: 3.952 ± 0.639
4.347GluVal: 4.347 ± 0.829
0.692GluTrp: 0.692 ± 0.273
1.976GluTyr: 1.976 ± 0.516
0.0GluXaa: 0.0 ± 0.0
Phe
2.667PheAla: 2.667 ± 0.58
0.099PheCys: 0.099 ± 0.086
3.655PheAsp: 3.655 ± 0.471
2.766PheGlu: 2.766 ± 0.633
1.186PhePhe: 1.186 ± 0.375
2.964PheGly: 2.964 ± 0.556
0.889PheHis: 0.889 ± 0.29
2.075PheIle: 2.075 ± 0.465
3.359PheLys: 3.359 ± 0.454
2.371PheLeu: 2.371 ± 0.403
1.383PheMet: 1.383 ± 0.374
2.667PheAsn: 2.667 ± 0.575
1.087PhePro: 1.087 ± 0.345
1.581PheGln: 1.581 ± 0.415
1.778PheArg: 1.778 ± 0.403
2.667PheSer: 2.667 ± 0.767
3.161PheThr: 3.161 ± 0.574
2.272PheVal: 2.272 ± 0.511
0.395PheTrp: 0.395 ± 0.202
1.68PheTyr: 1.68 ± 0.465
0.0PheXaa: 0.0 ± 0.0
Gly
4.149GlyAla: 4.149 ± 1.03
0.0GlyCys: 0.0 ± 0.0
3.359GlyAsp: 3.359 ± 0.578
3.063GlyGlu: 3.063 ± 0.482
3.655GlyPhe: 3.655 ± 0.574
4.248GlyGly: 4.248 ± 0.818
0.692GlyHis: 0.692 ± 0.22
6.817GlyIle: 6.817 ± 1.4
5.434GlyLys: 5.434 ± 0.773
4.94GlyLeu: 4.94 ± 0.653
1.383GlyMet: 1.383 ± 0.355
2.964GlyAsn: 2.964 ± 0.578
0.79GlyPro: 0.79 ± 0.391
2.766GlyGln: 2.766 ± 0.511
1.68GlyArg: 1.68 ± 0.38
4.149GlySer: 4.149 ± 0.832
4.545GlyThr: 4.545 ± 0.62
3.655GlyVal: 3.655 ± 0.55
0.988GlyTrp: 0.988 ± 0.297
3.359GlyTyr: 3.359 ± 0.585
0.0GlyXaa: 0.0 ± 0.0
His
1.284HisAla: 1.284 ± 0.552
0.0HisCys: 0.0 ± 0.0
0.988HisAsp: 0.988 ± 0.285
1.383HisGlu: 1.383 ± 0.31
0.395HisPhe: 0.395 ± 0.172
0.79HisGly: 0.79 ± 0.267
0.198HisHis: 0.198 ± 0.14
0.79HisIle: 0.79 ± 0.321
0.593HisLys: 0.593 ± 0.221
0.988HisLeu: 0.988 ± 0.346
0.395HisMet: 0.395 ± 0.193
0.79HisAsn: 0.79 ± 0.22
0.494HisPro: 0.494 ± 0.209
0.692HisGln: 0.692 ± 0.229
0.593HisArg: 0.593 ± 0.226
0.988HisSer: 0.988 ± 0.332
0.494HisThr: 0.494 ± 0.251
0.494HisVal: 0.494 ± 0.225
0.198HisTrp: 0.198 ± 0.138
0.889HisTyr: 0.889 ± 0.311
0.0HisXaa: 0.0 ± 0.0
Ile
4.94IleAla: 4.94 ± 0.666
0.296IleCys: 0.296 ± 0.183
5.73IleAsp: 5.73 ± 0.644
5.335IleGlu: 5.335 ± 1.053
1.778IlePhe: 1.778 ± 0.408
5.236IleGly: 5.236 ± 0.759
1.186IleHis: 1.186 ± 0.505
4.643IleIle: 4.643 ± 0.915
7.014IleLys: 7.014 ± 0.652
5.434IleLeu: 5.434 ± 0.812
1.482IleMet: 1.482 ± 0.404
4.149IleAsn: 4.149 ± 0.573
3.655IlePro: 3.655 ± 0.621
3.458IleGln: 3.458 ± 0.547
2.272IleArg: 2.272 ± 0.323
8.101IleSer: 8.101 ± 1.266
4.347IleThr: 4.347 ± 0.794
3.655IleVal: 3.655 ± 0.527
0.099IleTrp: 0.099 ± 0.094
1.778IleTyr: 1.778 ± 0.468
0.0IleXaa: 0.0 ± 0.0
Lys
6.026LysAla: 6.026 ± 0.574
0.296LysCys: 0.296 ± 0.17
4.841LysAsp: 4.841 ± 0.718
6.916LysGlu: 6.916 ± 0.986
3.063LysPhe: 3.063 ± 0.746
6.026LysGly: 6.026 ± 0.8
0.988LysHis: 0.988 ± 0.509
5.631LysIle: 5.631 ± 0.87
7.508LysLys: 7.508 ± 1.22
7.212LysLeu: 7.212 ± 0.78
1.976LysMet: 1.976 ± 0.483
5.829LysAsn: 5.829 ± 0.835
3.063LysPro: 3.063 ± 0.434
3.853LysGln: 3.853 ± 0.584
3.458LysArg: 3.458 ± 0.628
5.236LysSer: 5.236 ± 0.589
6.224LysThr: 6.224 ± 0.837
3.952LysVal: 3.952 ± 0.931
1.087LysTrp: 1.087 ± 0.309
3.063LysTyr: 3.063 ± 0.693
0.0LysXaa: 0.0 ± 0.0
Leu
5.137LeuAla: 5.137 ± 0.676
0.395LeuCys: 0.395 ± 0.199
4.545LeuAsp: 4.545 ± 0.718
5.533LeuGlu: 5.533 ± 0.773
3.359LeuPhe: 3.359 ± 0.569
5.137LeuGly: 5.137 ± 0.674
0.296LeuHis: 0.296 ± 0.152
4.742LeuIle: 4.742 ± 0.679
9.287LeuLys: 9.287 ± 1.046
5.434LeuLeu: 5.434 ± 0.789
2.173LeuMet: 2.173 ± 0.459
5.73LeuAsn: 5.73 ± 0.643
3.557LeuPro: 3.557 ± 0.542
3.26LeuGln: 3.26 ± 0.49
2.667LeuArg: 2.667 ± 0.573
6.125LeuSer: 6.125 ± 0.853
5.335LeuThr: 5.335 ± 0.7
3.557LeuVal: 3.557 ± 0.466
0.988LeuTrp: 0.988 ± 0.34
2.075LeuTyr: 2.075 ± 0.569
0.0LeuXaa: 0.0 ± 0.0
Met
1.778MetAla: 1.778 ± 0.39
0.198MetCys: 0.198 ± 0.143
1.087MetAsp: 1.087 ± 0.365
2.272MetGlu: 2.272 ± 0.479
0.395MetPhe: 0.395 ± 0.197
1.284MetGly: 1.284 ± 0.345
0.296MetHis: 0.296 ± 0.158
1.68MetIle: 1.68 ± 0.394
2.075MetLys: 2.075 ± 0.561
1.581MetLeu: 1.581 ± 0.374
0.593MetMet: 0.593 ± 0.222
1.383MetAsn: 1.383 ± 0.451
0.988MetPro: 0.988 ± 0.31
0.889MetGln: 0.889 ± 0.249
1.087MetArg: 1.087 ± 0.354
2.766MetSer: 2.766 ± 0.498
2.865MetThr: 2.865 ± 0.483
1.68MetVal: 1.68 ± 0.562
0.198MetTrp: 0.198 ± 0.129
0.79MetTyr: 0.79 ± 0.228
0.0MetXaa: 0.0 ± 0.0
Asn
3.359AsnAla: 3.359 ± 0.573
0.296AsnCys: 0.296 ± 0.147
3.557AsnAsp: 3.557 ± 0.578
3.161AsnGlu: 3.161 ± 0.573
3.26AsnPhe: 3.26 ± 0.499
3.655AsnGly: 3.655 ± 0.64
0.79AsnHis: 0.79 ± 0.417
4.446AsnIle: 4.446 ± 0.736
4.841AsnLys: 4.841 ± 0.682
4.051AsnLeu: 4.051 ± 0.738
0.988AsnMet: 0.988 ± 0.335
4.347AsnAsn: 4.347 ± 0.651
3.26AsnPro: 3.26 ± 0.495
3.359AsnGln: 3.359 ± 0.627
2.569AsnArg: 2.569 ± 0.542
3.853AsnSer: 3.853 ± 0.554
2.964AsnThr: 2.964 ± 0.555
3.754AsnVal: 3.754 ± 0.545
0.593AsnTrp: 0.593 ± 0.199
1.778AsnTyr: 1.778 ± 0.417
0.0AsnXaa: 0.0 ± 0.0
Pro
1.383ProAla: 1.383 ± 0.389
0.0ProCys: 0.0 ± 0.0
2.173ProAsp: 2.173 ± 0.574
2.075ProGlu: 2.075 ± 0.489
1.68ProPhe: 1.68 ± 0.385
0.988ProGly: 0.988 ± 0.294
0.296ProHis: 0.296 ± 0.175
2.964ProIle: 2.964 ± 0.524
3.063ProLys: 3.063 ± 0.532
3.853ProLeu: 3.853 ± 0.527
0.79ProMet: 0.79 ± 0.296
1.778ProAsn: 1.778 ± 0.393
0.395ProPro: 0.395 ± 0.176
1.186ProGln: 1.186 ± 0.337
1.284ProArg: 1.284 ± 0.32
2.272ProSer: 2.272 ± 0.389
1.976ProThr: 1.976 ± 0.375
1.976ProVal: 1.976 ± 0.46
0.296ProTrp: 0.296 ± 0.15
0.692ProTyr: 0.692 ± 0.274
0.0ProXaa: 0.0 ± 0.0
Gln
4.841GlnAla: 4.841 ± 0.743
0.099GlnCys: 0.099 ± 0.092
2.075GlnAsp: 2.075 ± 0.532
4.051GlnGlu: 4.051 ± 0.676
1.68GlnPhe: 1.68 ± 0.423
1.581GlnGly: 1.581 ± 0.369
0.593GlnHis: 0.593 ± 0.259
2.47GlnIle: 2.47 ± 0.481
4.545GlnLys: 4.545 ± 0.74
3.952GlnLeu: 3.952 ± 0.653
0.79GlnMet: 0.79 ± 0.306
2.47GlnAsn: 2.47 ± 0.582
1.087GlnPro: 1.087 ± 0.281
1.581GlnGln: 1.581 ± 0.464
1.778GlnArg: 1.778 ± 0.319
2.865GlnSer: 2.865 ± 0.797
1.976GlnThr: 1.976 ± 0.471
1.877GlnVal: 1.877 ± 0.487
0.692GlnTrp: 0.692 ± 0.261
1.68GlnTyr: 1.68 ± 0.435
0.0GlnXaa: 0.0 ± 0.0
Arg
2.667ArgAla: 2.667 ± 0.458
0.296ArgCys: 0.296 ± 0.203
2.865ArgAsp: 2.865 ± 0.567
1.877ArgGlu: 1.877 ± 0.397
1.778ArgPhe: 1.778 ± 0.398
1.383ArgGly: 1.383 ± 0.43
0.296ArgHis: 0.296 ± 0.141
3.557ArgIle: 3.557 ± 0.617
3.557ArgLys: 3.557 ± 0.819
3.853ArgLeu: 3.853 ± 0.642
0.692ArgMet: 0.692 ± 0.285
2.371ArgAsn: 2.371 ± 0.495
0.889ArgPro: 0.889 ± 0.328
1.68ArgGln: 1.68 ± 0.399
1.976ArgArg: 1.976 ± 0.517
2.47ArgSer: 2.47 ± 0.551
2.766ArgThr: 2.766 ± 0.508
1.68ArgVal: 1.68 ± 0.495
0.494ArgTrp: 0.494 ± 0.237
1.68ArgTyr: 1.68 ± 0.511
0.0ArgXaa: 0.0 ± 0.0
Ser
5.829SerAla: 5.829 ± 0.986
0.198SerCys: 0.198 ± 0.16
4.248SerAsp: 4.248 ± 0.543
4.94SerGlu: 4.94 ± 0.614
2.272SerPhe: 2.272 ± 0.511
6.817SerGly: 6.817 ± 1.476
0.692SerHis: 0.692 ± 0.246
5.039SerIle: 5.039 ± 0.781
6.422SerLys: 6.422 ± 0.72
5.039SerLeu: 5.039 ± 0.949
2.173SerMet: 2.173 ± 0.488
3.754SerAsn: 3.754 ± 0.935
1.778SerPro: 1.778 ± 0.4
2.569SerGln: 2.569 ± 0.503
2.371SerArg: 2.371 ± 0.487
5.73SerSer: 5.73 ± 1.011
4.742SerThr: 4.742 ± 0.954
4.149SerVal: 4.149 ± 0.59
0.889SerTrp: 0.889 ± 0.312
3.161SerTyr: 3.161 ± 0.664
0.0SerXaa: 0.0 ± 0.0
Thr
3.952ThrAla: 3.952 ± 0.866
0.198ThrCys: 0.198 ± 0.228
4.051ThrAsp: 4.051 ± 0.8
4.347ThrGlu: 4.347 ± 0.635
2.173ThrPhe: 2.173 ± 0.441
5.335ThrGly: 5.335 ± 0.808
0.988ThrHis: 0.988 ± 0.41
5.829ThrIle: 5.829 ± 0.922
4.446ThrLys: 4.446 ± 0.703
4.94ThrLeu: 4.94 ± 0.775
1.383ThrMet: 1.383 ± 0.38
2.865ThrAsn: 2.865 ± 0.467
1.778ThrPro: 1.778 ± 0.391
2.865ThrGln: 2.865 ± 0.562
1.778ThrArg: 1.778 ± 0.476
3.952ThrSer: 3.952 ± 0.884
3.952ThrThr: 3.952 ± 0.724
5.829ThrVal: 5.829 ± 0.752
0.988ThrTrp: 0.988 ± 0.298
1.976ThrTyr: 1.976 ± 0.474
0.0ThrXaa: 0.0 ± 0.0
Val
2.964ValAla: 2.964 ± 0.555
0.494ValCys: 0.494 ± 0.248
4.643ValAsp: 4.643 ± 0.649
4.545ValGlu: 4.545 ± 0.804
2.272ValPhe: 2.272 ± 0.451
3.161ValGly: 3.161 ± 0.504
0.692ValHis: 0.692 ± 0.255
4.94ValIle: 4.94 ± 0.759
3.853ValLys: 3.853 ± 0.431
4.446ValLeu: 4.446 ± 0.714
1.482ValMet: 1.482 ± 0.356
2.766ValAsn: 2.766 ± 0.468
2.173ValPro: 2.173 ± 0.504
1.383ValGln: 1.383 ± 0.303
2.569ValArg: 2.569 ± 0.437
4.347ValSer: 4.347 ± 0.671
3.754ValThr: 3.754 ± 0.767
5.039ValVal: 5.039 ± 0.747
0.494ValTrp: 0.494 ± 0.222
2.173ValTyr: 2.173 ± 0.392
0.0ValXaa: 0.0 ± 0.0
Trp
0.296TrpAla: 0.296 ± 0.2
0.198TrpCys: 0.198 ± 0.156
0.692TrpAsp: 0.692 ± 0.256
0.79TrpGlu: 0.79 ± 0.24
0.494TrpPhe: 0.494 ± 0.216
0.494TrpGly: 0.494 ± 0.366
0.0TrpHis: 0.0 ± 0.0
0.988TrpIle: 0.988 ± 0.275
0.593TrpLys: 0.593 ± 0.24
0.988TrpLeu: 0.988 ± 0.319
0.099TrpMet: 0.099 ± 0.143
0.79TrpAsn: 0.79 ± 0.255
0.198TrpPro: 0.198 ± 0.127
0.79TrpGln: 0.79 ± 0.234
0.494TrpArg: 0.494 ± 0.2
1.383TrpSer: 1.383 ± 0.337
0.593TrpThr: 0.593 ± 0.259
1.087TrpVal: 1.087 ± 0.381
0.198TrpTrp: 0.198 ± 0.137
0.494TrpTyr: 0.494 ± 0.205
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.877TyrAla: 1.877 ± 0.415
0.0TyrCys: 0.0 ± 0.0
2.865TyrAsp: 2.865 ± 0.524
2.766TyrGlu: 2.766 ± 0.534
2.075TyrPhe: 2.075 ± 0.511
1.976TyrGly: 1.976 ± 0.432
0.692TyrHis: 0.692 ± 0.265
2.173TyrIle: 2.173 ± 0.633
2.865TyrLys: 2.865 ± 0.603
2.766TyrLeu: 2.766 ± 0.594
1.383TyrMet: 1.383 ± 0.348
1.383TyrAsn: 1.383 ± 0.344
0.889TyrPro: 0.889 ± 0.318
1.976TyrGln: 1.976 ± 0.416
1.877TyrArg: 1.877 ± 0.467
2.964TyrSer: 2.964 ± 0.552
1.778TyrThr: 1.778 ± 0.37
1.482TyrVal: 1.482 ± 0.292
0.494TyrTrp: 0.494 ± 0.195
1.383TyrTyr: 1.383 ± 0.412
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 50 proteins (10123 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski