Alkalibacterium sp. AK22

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Carnobacteriaceae; Alkalibacterium; unclassified Alkalibacterium

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2182 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A011RP42|A0A011RP42_9LACT Undecaprenyl-diphosphatase OS=Alkalibacterium sp. AK22 OX=1229520 GN=uppP PE=3 SV=1
MM1 pKa = 6.79NSKK4 pKa = 9.44WIKK7 pKa = 9.79LSSVALLSLYY17 pKa = 10.54LAACDD22 pKa = 3.38AAEE25 pKa = 4.52EE26 pKa = 4.17EE27 pKa = 4.67TADD30 pKa = 3.73EE31 pKa = 4.32EE32 pKa = 4.64TVEE35 pKa = 4.29VVDD38 pKa = 5.75DD39 pKa = 3.87SSEE42 pKa = 4.55ADD44 pKa = 3.71DD45 pKa = 5.39HH46 pKa = 7.39DD47 pKa = 6.18HH48 pKa = 8.0DD49 pKa = 6.77DD50 pKa = 3.81EE51 pKa = 6.09DD52 pKa = 5.26HH53 pKa = 7.34DD54 pKa = 5.32HH55 pKa = 7.26DD56 pKa = 6.28HH57 pKa = 7.89DD58 pKa = 6.77DD59 pKa = 3.73EE60 pKa = 5.94DD61 pKa = 5.26HH62 pKa = 7.34DD63 pKa = 5.32HH64 pKa = 7.26DD65 pKa = 6.28HH66 pKa = 7.89DD67 pKa = 6.77DD68 pKa = 3.73EE69 pKa = 5.94DD70 pKa = 5.26HH71 pKa = 7.34DD72 pKa = 5.32HH73 pKa = 7.26DD74 pKa = 6.28HH75 pKa = 7.89DD76 pKa = 6.77DD77 pKa = 3.73EE78 pKa = 5.94DD79 pKa = 5.26HH80 pKa = 7.34DD81 pKa = 5.32HH82 pKa = 7.26DD83 pKa = 6.28HH84 pKa = 7.89DD85 pKa = 6.77DD86 pKa = 3.73EE87 pKa = 5.96DD88 pKa = 4.88HH89 pKa = 7.12DD90 pKa = 4.65HH91 pKa = 7.1DD92 pKa = 4.75HH93 pKa = 6.72AHH95 pKa = 6.84GEE97 pKa = 3.99ADD99 pKa = 3.45VQEE102 pKa = 4.33TVEE105 pKa = 3.99IEE107 pKa = 4.78GISHH111 pKa = 7.21HH112 pKa = 5.76YY113 pKa = 8.38HH114 pKa = 5.36TGEE117 pKa = 3.95LVEE120 pKa = 4.19WTAVIEE126 pKa = 4.57DD127 pKa = 4.01DD128 pKa = 4.5NGYY131 pKa = 10.72DD132 pKa = 3.31DD133 pKa = 3.54WHH135 pKa = 7.14WYY137 pKa = 7.67MRR139 pKa = 11.84ADD141 pKa = 5.63DD142 pKa = 3.98SADD145 pKa = 2.87WEE147 pKa = 4.67MISGQHH153 pKa = 5.56SEE155 pKa = 4.18EE156 pKa = 4.33LVFEE160 pKa = 4.43APEE163 pKa = 3.57EE164 pKa = 4.26SMEE167 pKa = 4.16IKK169 pKa = 10.58AVLYY173 pKa = 10.72DD174 pKa = 3.98DD175 pKa = 4.87AHH177 pKa = 6.84DD178 pKa = 4.93AFAQSAAVEE187 pKa = 4.32LEE189 pKa = 4.13VDD191 pKa = 3.29NHH193 pKa = 5.7

Molecular weight: 22.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A011RM66|A0A011RM66_9LACT 2' 3'-cyclic-nucleotide 2'-phosphodiesterase OS=Alkalibacterium sp. AK22 OX=1229520 GN=ADIAL_1566 PE=3 SV=1
MM1 pKa = 8.12DD2 pKa = 6.46IIRR5 pKa = 11.84LLQLIVSSSLIFAAPLILTALGGTFSEE32 pKa = 4.59RR33 pKa = 11.84SGVVNIGLEE42 pKa = 4.62GIMVMGAFMSAIFNIQFVGTFGNMTPWVGMLAGGLIGLLFSIIHH86 pKa = 6.24AVATINLRR94 pKa = 11.84ADD96 pKa = 4.14HH97 pKa = 6.52IVSGTVINLAAPALAVFLTRR117 pKa = 11.84ALFGAAQTGPLPRR130 pKa = 11.84SFGRR134 pKa = 11.84SNIFLLHH141 pKa = 7.32RR142 pKa = 11.84IPVIGPIFFRR152 pKa = 11.84NTSGPAFFGILMAILSWFILYY173 pKa = 7.71KK174 pKa = 10.27TRR176 pKa = 11.84FGLRR180 pKa = 11.84LRR182 pKa = 11.84SVGEE186 pKa = 4.07HH187 pKa = 5.12PHH189 pKa = 5.97AAEE192 pKa = 3.77TLGIKK197 pKa = 10.07VYY199 pKa = 7.59TMKK202 pKa = 10.85YY203 pKa = 9.96AGVLISGFLGGMGGAIQSQAIHH225 pKa = 6.37NEE227 pKa = 3.72FGILTVAGQGFIAIAAMIFGKK248 pKa = 9.56WNPLGAMGAAIFFGFAQSLARR269 pKa = 11.84VGNSIPIIQNIPSVWLQVLPYY290 pKa = 10.35LLTILVLVGFIGRR303 pKa = 11.84AEE305 pKa = 3.96APAAIGKK312 pKa = 7.92TYY314 pKa = 10.36IKK316 pKa = 10.71SKK318 pKa = 10.9

Molecular weight: 33.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2182

0

2182

655076

37

1531

300.2

33.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.504 ± 0.052

0.515 ± 0.013

5.843 ± 0.045

7.671 ± 0.073

4.372 ± 0.048

6.868 ± 0.053

2.011 ± 0.028

7.031 ± 0.044

6.031 ± 0.054

9.995 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.769 ± 0.024

3.981 ± 0.036

3.48 ± 0.031

3.993 ± 0.039

4.456 ± 0.038

6.352 ± 0.039

5.677 ± 0.033

7.106 ± 0.045

0.903 ± 0.018

3.442 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski