Meles meles polyomavirus 1
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B5L789|A0A0B5L789_9POLY Small t antigen OS=Meles meles polyomavirus 1 OX=1608323 PE=4 SV=1
MM1 pKa = 7.5 KK2 pKa = 9.06 RR3 pKa = 11.84 TLPKK7 pKa = 10.23 RR8 pKa = 11.84 SGSKK12 pKa = 9.97 GAAPAQVPKK21 pKa = 10.83 LLIKK25 pKa = 10.76 GGIEE29 pKa = 3.68 VLEE32 pKa = 4.23 IRR34 pKa = 11.84 TGADD38 pKa = 3.06 SITTIEE44 pKa = 4.53 LFLNPRR50 pKa = 11.84 TGTPSEE56 pKa = 4.41 HH57 pKa = 6.35 EE58 pKa = 4.28 FGYY61 pKa = 10.2 SANITVATTDD71 pKa = 3.28 TADD74 pKa = 3.37 KK75 pKa = 10.59 PKK77 pKa = 9.98 IDD79 pKa = 4.29 EE80 pKa = 4.67 LPCFSVGKK88 pKa = 10.64 VKK90 pKa = 10.86 LPLLNDD96 pKa = 3.76 DD97 pKa = 3.7 MTGDD101 pKa = 4.67 KK102 pKa = 10.6 ILMWEE107 pKa = 4.06 AVSCKK112 pKa = 10.06 TEE114 pKa = 4.06 VIGAGSLCNVHH125 pKa = 6.22 SFRR128 pKa = 11.84 KK129 pKa = 9.69 RR130 pKa = 11.84 VDD132 pKa = 3.19 NGSYY136 pKa = 9.08 TAAQPIVGLNYY147 pKa = 10.52 HH148 pKa = 6.26 MFAVGGEE155 pKa = 3.91 PLQLQFLTEE164 pKa = 4.37 NYY166 pKa = 6.05 QTSYY170 pKa = 9.95 KK171 pKa = 10.55 DD172 pKa = 3.19 SYY174 pKa = 10.88 AAPKK178 pKa = 10.53 VPLTGLAQVLDD189 pKa = 4.04 PRR191 pKa = 11.84 LKK193 pKa = 11.04 GILDD197 pKa = 3.34 KK198 pKa = 11.25 DD199 pKa = 3.52 GYY201 pKa = 11.08 YY202 pKa = 9.8 PVEE205 pKa = 4.4 CWHH208 pKa = 7.34 PDD210 pKa = 3.19 PAKK213 pKa = 10.75 NEE215 pKa = 3.62 NSRR218 pKa = 11.84 YY219 pKa = 9.33 FGTYY223 pKa = 8.13 TGGVQTPPVLQFSNSLTTILLDD245 pKa = 3.85 EE246 pKa = 5.02 NGVGPLCKK254 pKa = 10.05 GDD256 pKa = 4.52 NLFLSCCDD264 pKa = 3.36 VVGFKK269 pKa = 10.89 YY270 pKa = 10.74 EE271 pKa = 3.83 SGGTMKK277 pKa = 10.27 FRR279 pKa = 11.84 GLPRR283 pKa = 11.84 YY284 pKa = 9.63 FSVTLRR290 pKa = 11.84 KK291 pKa = 9.58 RR292 pKa = 11.84 QVKK295 pKa = 9.74 NPYY298 pKa = 9.5 PVGTLLNSLFNKK310 pKa = 9.28 LAPDD314 pKa = 3.58 VDD316 pKa = 4.38 SQPMTGEE323 pKa = 3.8 HH324 pKa = 5.67 SQVEE328 pKa = 4.56 EE329 pKa = 3.78 VRR331 pKa = 11.84 VYY333 pKa = 10.74 QGTEE337 pKa = 3.88 VLPGDD342 pKa = 4.76 PDD344 pKa = 3.42 LQRR347 pKa = 11.84 YY348 pKa = 7.81 VDD350 pKa = 3.59 QYY352 pKa = 9.61 GQKK355 pKa = 8.01 EE356 pKa = 4.36 TRR358 pKa = 11.84 PPQDD362 pKa = 2.97 TEE364 pKa = 4.48 RR365 pKa = 11.84 NLLLEE370 pKa = 4.13 INKK373 pKa = 9.86 LKK375 pKa = 10.94 ARR377 pKa = 11.84 LQYY380 pKa = 10.48 VRR382 pKa = 11.84 QKK384 pKa = 10.89 RR385 pKa = 11.84 PDD387 pKa = 3.84 PPQIMYY393 pKa = 9.4 TPFPYY398 pKa = 10.09 VEE400 pKa = 3.85 RR401 pKa = 11.84 NGRR404 pKa = 11.84 HH405 pKa = 4.34 VVIPTPDD412 pKa = 3.31 GGLTGHH418 pKa = 6.94 IEE420 pKa = 4.57 GIIEE424 pKa = 4.03 TEE426 pKa = 4.61 EE427 pKa = 3.91 ISEE430 pKa = 4.33 APPPPSPSSAPLPPPTPTATPIVLPHH456 pKa = 6.15 PTVEE460 pKa = 4.36 LTGHH464 pKa = 6.88 PVTEE468 pKa = 4.72 EE469 pKa = 4.22 DD470 pKa = 4.4 NEE472 pKa = 4.54 SPSDD476 pKa = 3.87 GDD478 pKa = 3.67 PSHH481 pKa = 6.74 ATTSNVIEE489 pKa = 4.38 FTMDD493 pKa = 3.02 TASS496 pKa = 3.07
Molecular weight: 54.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.23
IPC2_protein 5.283
IPC_protein 5.27
Toseland 5.334
ProMoST 5.461
Dawson 5.321
Bjellqvist 5.423
Wikipedia 5.232
Rodwell 5.258
Grimsley 5.308
Solomon 5.321
Lehninger 5.296
Nozaki 5.486
DTASelect 5.639
Thurlkill 5.397
EMBOSS 5.347
Sillero 5.563
Patrickios 3.986
IPC_peptide 5.334
IPC2_peptide 5.563
IPC2.peptide.svr19 5.536
Protein with the highest isoelectric point:
>tr|A0A0B5L589|A0A0B5L589_9POLY Major capsid protein VP1 OS=Meles meles polyomavirus 1 OX=1608323 PE=3 SV=1
MM1 pKa = 7.65 ALQVWMPQIDD11 pKa = 3.92 YY12 pKa = 9.96 LFPGFSTFARR22 pKa = 11.84 YY23 pKa = 8.28 AQYY26 pKa = 11.09 LDD28 pKa = 3.68 PFVWGPDD35 pKa = 3.78 LINHH39 pKa = 5.86 VSRR42 pKa = 11.84 LFWQALLSEE51 pKa = 4.57 GRR53 pKa = 11.84 RR54 pKa = 11.84 QIGYY58 pKa = 9.61 AARR61 pKa = 11.84 DD62 pKa = 3.43 LQAASRR68 pKa = 11.84 EE69 pKa = 4.25 LATRR73 pKa = 11.84 TATEE77 pKa = 3.85 VAGTVQDD84 pKa = 3.44 AVARR88 pKa = 11.84 FFEE91 pKa = 4.22 NTRR94 pKa = 11.84 WAVSHH99 pKa = 5.25 VTTPYY104 pKa = 10.96 GALQNYY110 pKa = 7.67 YY111 pKa = 10.27 RR112 pKa = 11.84 QLSPVNPPQLRR123 pKa = 11.84 QLEE126 pKa = 4.16 RR127 pKa = 11.84 RR128 pKa = 11.84 LTDD131 pKa = 2.81 RR132 pKa = 11.84 GVDD135 pKa = 3.07 IRR137 pKa = 11.84 QDD139 pKa = 3.6 EE140 pKa = 4.52 EE141 pKa = 3.8 KK142 pKa = 9.89 HH143 pKa = 5.75 YY144 pKa = 10.8 PSAEE148 pKa = 4.04 YY149 pKa = 9.89 IEE151 pKa = 5.61 KK152 pKa = 10.68 YY153 pKa = 9.68 GAPGGAGQRR162 pKa = 11.84 HH163 pKa = 5.43 APDD166 pKa = 3.09 WLLPLLLGLYY176 pKa = 10.72 GDD178 pKa = 5.21 LTPTWRR184 pKa = 11.84 KK185 pKa = 9.5 EE186 pKa = 3.46 IEE188 pKa = 4.09 EE189 pKa = 4.11 QIEE192 pKa = 4.34 SEE194 pKa = 4.41 DD195 pKa = 5.26 EE196 pKa = 3.68 EE197 pKa = 4.47 DD198 pKa = 3.67 TPQKK202 pKa = 10.79 KK203 pKa = 8.96 RR204 pKa = 11.84 VKK206 pKa = 10.1 RR207 pKa = 11.84 SRR209 pKa = 11.84 PSPGAKK215 pKa = 9.03 ATDD218 pKa = 3.53 KK219 pKa = 10.56 RR220 pKa = 11.84 RR221 pKa = 11.84 HH222 pKa = 5.5 RR223 pKa = 11.84 STRR226 pKa = 11.84 NKK228 pKa = 9.82 NRR230 pKa = 11.84 SRR232 pKa = 3.54
Molecular weight: 26.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.863
IPC2_protein 9.033
IPC_protein 9.18
Toseland 9.428
ProMoST 9.458
Dawson 9.765
Bjellqvist 9.516
Wikipedia 10.014
Rodwell 9.867
Grimsley 9.882
Solomon 9.823
Lehninger 9.765
Nozaki 9.385
DTASelect 9.516
Thurlkill 9.56
EMBOSS 9.867
Sillero 9.663
Patrickios 4.902
IPC_peptide 9.809
IPC2_peptide 8.156
IPC2.peptide.svr19 7.952
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1936
178
681
387.2
43.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.457 ± 1.323
1.86 ± 0.664
5.579 ± 0.309
7.541 ± 0.408
3.667 ± 0.304
6.973 ± 0.381
1.86 ± 0.066
4.184 ± 0.293
5.992 ± 1.082
10.072 ± 0.543
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.014 ± 0.442
3.719 ± 0.44
6.147 ± 1.227
4.494 ± 0.348
5.888 ± 1.099
6.508 ± 0.353
6.147 ± 0.714
5.424 ± 0.526
1.601 ± 0.457
3.874 ± 0.166
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here