Torque teno midi virus 10
Average proteome isoelectric point is 7.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A7VLY8|A7VLY8_9VIRU Capsid protein OS=Torque teno midi virus 10 OX=2065051 PE=3 SV=1
MM1 pKa = 7.43 SRR3 pKa = 11.84 FYY5 pKa = 10.76 TQTKK9 pKa = 10.01 YY10 pKa = 11.1 NGATLNQMWMSMIADD25 pKa = 3.69 SHH27 pKa = 7.49 DD28 pKa = 3.11 IHH30 pKa = 7.35 CDD32 pKa = 3.16 CKK34 pKa = 11.08 KK35 pKa = 10.52 PFAHH39 pKa = 6.99 LLDD42 pKa = 4.78 NIFPEE47 pKa = 4.38 GHH49 pKa = 5.69 TDD51 pKa = 3.31 RR52 pKa = 11.84 NKK54 pKa = 9.94 TVAHH58 pKa = 7.21 IINRR62 pKa = 11.84 DD63 pKa = 3.44 YY64 pKa = 11.26 KK65 pKa = 8.88 EE66 pKa = 4.21 CLSGGEE72 pKa = 4.43 EE73 pKa = 4.25 EE74 pKa = 5.44 DD75 pKa = 4.74 DD76 pKa = 3.87 PGIPVGASAATAAGDD91 pKa = 3.78 TEE93 pKa = 4.51 IKK95 pKa = 10.71 LEE97 pKa = 3.95 EE98 pKa = 4.41 HH99 pKa = 6.57 LEE101 pKa = 3.96 EE102 pKa = 4.73 DD103 pKa = 3.45 AEE105 pKa = 4.38 EE106 pKa = 4.24 IEE108 pKa = 4.52 KK109 pKa = 10.96 LLAAAEE115 pKa = 4.12 EE116 pKa = 4.46 NYY118 pKa = 9.97 IRR120 pKa = 5.2
Molecular weight: 13.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.553
IPC2_protein 4.609
IPC_protein 4.52
Toseland 4.393
ProMoST 4.635
Dawson 4.469
Bjellqvist 4.622
Wikipedia 4.342
Rodwell 4.38
Grimsley 4.304
Solomon 4.469
Lehninger 4.431
Nozaki 4.584
DTASelect 4.724
Thurlkill 4.393
EMBOSS 4.355
Sillero 4.647
Patrickios 3.579
IPC_peptide 4.482
IPC2_peptide 4.647
IPC2.peptide.svr19 4.568
Protein with the highest isoelectric point:
>tr|A7VLY8|A7VLY8_9VIRU Capsid protein OS=Torque teno midi virus 10 OX=2065051 PE=3 SV=1
MM1 pKa = 7.33 PFWWRR6 pKa = 11.84 RR7 pKa = 11.84 RR8 pKa = 11.84 RR9 pKa = 11.84 RR10 pKa = 11.84 PWYY13 pKa = 8.27 TRR15 pKa = 11.84 WGQRR19 pKa = 11.84 RR20 pKa = 11.84 YY21 pKa = 9.74 RR22 pKa = 11.84 RR23 pKa = 11.84 RR24 pKa = 11.84 RR25 pKa = 11.84 YY26 pKa = 9.09 RR27 pKa = 11.84 NKK29 pKa = 8.86 TRR31 pKa = 11.84 RR32 pKa = 11.84 TFGRR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 RR40 pKa = 11.84 NRR42 pKa = 11.84 KK43 pKa = 7.87 APRR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 RR49 pKa = 11.84 RR50 pKa = 11.84 KK51 pKa = 7.83 LHH53 pKa = 4.89 KK54 pKa = 10.07 VRR56 pKa = 11.84 RR57 pKa = 11.84 KK58 pKa = 10.08 KK59 pKa = 10.72 KK60 pKa = 9.68 FLKK63 pKa = 10.15 LVQWQPDD70 pKa = 4.08 RR71 pKa = 11.84 IRR73 pKa = 11.84 KK74 pKa = 7.59 CTIKK78 pKa = 10.81 GRR80 pKa = 11.84 GTLVMGAHH88 pKa = 6.8 GRR90 pKa = 11.84 QMICFTNVKK99 pKa = 10.31 DD100 pKa = 3.54 RR101 pKa = 11.84 LTPSRR106 pKa = 11.84 APAGGGFGVEE116 pKa = 4.65 RR117 pKa = 11.84 ITLAHH122 pKa = 7.34 LYY124 pKa = 9.98 EE125 pKa = 4.18 EE126 pKa = 4.33 WKK128 pKa = 10.74 ARR130 pKa = 11.84 NNVWTATNEE139 pKa = 3.87 NTDD142 pKa = 3.24 LGRR145 pKa = 11.84 YY146 pKa = 5.95 TGCKK150 pKa = 8.51 ISFYY154 pKa = 10.79 RR155 pKa = 11.84 HH156 pKa = 6.73 LDD158 pKa = 2.84 TDD160 pKa = 4.18 FIIKK164 pKa = 10.13 YY165 pKa = 9.96 SRR167 pKa = 11.84 NPPFNLEE174 pKa = 4.22 KK175 pKa = 10.5 YY176 pKa = 10.2 SYY178 pKa = 10.41 MYY180 pKa = 7.97 MQPQEE185 pKa = 4.32 LLLGKK190 pKa = 9.62 HH191 pKa = 5.6 KK192 pKa = 10.73 RR193 pKa = 11.84 ILLSKK198 pKa = 9.01 KK199 pKa = 8.17 TNPKK203 pKa = 10.32 GKK205 pKa = 10.4 LKK207 pKa = 8.36 TTLKK211 pKa = 10.34 IGPPKK216 pKa = 10.73 LLTNKK221 pKa = 9.08 WLLQSEE227 pKa = 4.56 LAKK230 pKa = 10.26 QDD232 pKa = 3.6 LVEE235 pKa = 4.53 ISAAAASFTYY245 pKa = 8.25 PTIGCCNEE253 pKa = 3.28 NRR255 pKa = 11.84 ILNLYY260 pKa = 9.89 SINTEE265 pKa = 4.27 FYY267 pKa = 11.07 KK268 pKa = 10.61 DD269 pKa = 3.64 AHH271 pKa = 6.15 WMQAHH276 pKa = 7.31 AGTNYY281 pKa = 9.14 YY282 pKa = 10.17 KK283 pKa = 10.23 PYY285 pKa = 9.56 STIPSDD291 pKa = 4.58 LKK293 pKa = 10.7 FHH295 pKa = 6.81 YY296 pKa = 8.18 KK297 pKa = 7.68 TQSGGEE303 pKa = 4.14 SAINGPDD310 pKa = 3.5 SKK312 pKa = 11.17 EE313 pKa = 3.74 KK314 pKa = 10.9 ALTLEE319 pKa = 4.82 GGWFQPKK326 pKa = 9.74 ILGAYY331 pKa = 9.25 KK332 pKa = 8.79 ITKK335 pKa = 9.93 GSTITQYY342 pKa = 11.76 GEE344 pKa = 3.96 LPMVLLRR351 pKa = 11.84 YY352 pKa = 9.87 NPAADD357 pKa = 3.49 QGPGNIVTIIDD368 pKa = 4.06 LVSGQWQGPYY378 pKa = 9.9 PDD380 pKa = 5.72 DD381 pKa = 3.63 YY382 pKa = 10.83 TIKK385 pKa = 10.68 DD386 pKa = 3.61 VPLWLALNGYY396 pKa = 8.72 PNYY399 pKa = 10.43 VMQKK403 pKa = 10.0 SGDD406 pKa = 3.56 KK407 pKa = 11.05 GILKK411 pKa = 10.33 HH412 pKa = 7.01 KK413 pKa = 9.0 LICVKK418 pKa = 10.45 SAALYY423 pKa = 9.64 RR424 pKa = 11.84 LPTVSTQKK432 pKa = 9.72 IFPIIGVKK440 pKa = 9.76 FVNGKK445 pKa = 8.93 PPYY448 pKa = 9.0 DD449 pKa = 3.56 QPLTSDD455 pKa = 3.9 MSKK458 pKa = 10.13 AWYY461 pKa = 8.61 PIYY464 pKa = 10.22 EE465 pKa = 4.31 HH466 pKa = 5.75 QQEE469 pKa = 4.31 PLNDD473 pKa = 3.66 IVCCGPLVPKK483 pKa = 10.24 FYY485 pKa = 10.96 NQTNSTWEE493 pKa = 3.56 LQYY496 pKa = 10.64 FYY498 pKa = 11.03 RR499 pKa = 11.84 FYY501 pKa = 10.8 FKK503 pKa = 10.24 WGGPEE508 pKa = 3.74 IGDD511 pKa = 3.82 PTVDD515 pKa = 3.66 DD516 pKa = 5.13 PKK518 pKa = 10.97 TKK520 pKa = 10.47 GQATTDD526 pKa = 3.41 YY527 pKa = 10.04 TMPRR531 pKa = 11.84 SIQVFNPLKK540 pKa = 10.77 NKK542 pKa = 9.32 TEE544 pKa = 4.66 SILHH548 pKa = 5.88 PWDD551 pKa = 3.57 FRR553 pKa = 11.84 RR554 pKa = 11.84 GIVTKK559 pKa = 10.42 RR560 pKa = 11.84 ALEE563 pKa = 3.96 RR564 pKa = 11.84 MCQNLEE570 pKa = 4.0 TDD572 pKa = 3.7 SNFYY576 pKa = 10.38 PDD578 pKa = 4.76 GEE580 pKa = 4.53 TPKK583 pKa = 10.4 KK584 pKa = 10.08 KK585 pKa = 10.45 KK586 pKa = 9.18 KK587 pKa = 5.61 TTCEE591 pKa = 3.44 LRR593 pKa = 11.84 NPQEE597 pKa = 4.24 EE598 pKa = 4.21 IKK600 pKa = 10.47 EE601 pKa = 4.15 VQSCILSLCQTEE613 pKa = 4.37 TSEE616 pKa = 5.61 EE617 pKa = 4.03 EE618 pKa = 4.37 DD619 pKa = 3.94 PNNLQQLIHH628 pKa = 5.91 KK629 pKa = 9.86 QKK631 pKa = 10.85 LKK633 pKa = 10.14 QQQLKK638 pKa = 10.82 HH639 pKa = 6.35 NILVLLTDD647 pKa = 5.09 LKK649 pKa = 10.33 TKK651 pKa = 10.54 QNSLRR656 pKa = 11.84 LQTGNLQQ663 pKa = 3.29
Molecular weight: 77.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.182
IPC2_protein 9.414
IPC_protein 9.399
Toseland 10.072
ProMoST 9.75
Dawson 10.277
Bjellqvist 9.94
Wikipedia 10.438
Rodwell 10.745
Grimsley 10.335
Solomon 10.292
Lehninger 10.248
Nozaki 10.087
DTASelect 9.926
Thurlkill 10.131
EMBOSS 10.482
Sillero 10.189
Patrickios 10.262
IPC_peptide 10.292
IPC2_peptide 8.639
IPC2.peptide.svr19 8.333
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1297
120
663
324.3
37.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.708 ± 1.867
1.928 ± 0.095
4.703 ± 0.587
6.862 ± 1.541
2.93 ± 0.376
5.32 ± 0.715
3.007 ± 0.677
5.628 ± 0.258
9.098 ± 0.957
8.096 ± 0.819
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.005 ± 0.251
5.012 ± 0.614
6.322 ± 0.736
5.166 ± 0.49
6.322 ± 0.915
5.628 ± 1.619
7.17 ± 0.459
2.39 ± 0.709
1.619 ± 0.52
4.086 ± 0.688
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here