Curionopolis virus
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A068J4Q8|A0A068J4Q8_9RHAB Nucleocapsid protein OS=Curionopolis virus OX=490110 GN=N PE=4 SV=1
MM1 pKa = 7.62 KK2 pKa = 10.62 SLGDD6 pKa = 3.44 WLFQYY11 pKa = 11.16 SLDD14 pKa = 3.28 RR15 pKa = 11.84 SLFVTRR21 pKa = 11.84 MFEE24 pKa = 4.29 SEE26 pKa = 4.39 EE27 pKa = 4.11 EE28 pKa = 4.03 FLDD31 pKa = 4.75 FYY33 pKa = 11.53 VDD35 pKa = 5.04 LYY37 pKa = 10.57 IACKK41 pKa = 9.99 SVNEE45 pKa = 4.57 SIHH48 pKa = 4.58 VTVTRR53 pKa = 11.84 SLIDD57 pKa = 3.59 PFHH60 pKa = 7.14 VIFRR64 pKa = 11.84 IWQTT68 pKa = 3.04
Molecular weight: 8.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.8
IPC2_protein 4.94
IPC_protein 4.736
Toseland 4.622
ProMoST 4.863
Dawson 4.711
Bjellqvist 4.851
Wikipedia 4.596
Rodwell 4.609
Grimsley 4.546
Solomon 4.698
Lehninger 4.66
Nozaki 4.838
DTASelect 4.991
Thurlkill 4.635
EMBOSS 4.622
Sillero 4.889
Patrickios 3.77
IPC_peptide 4.711
IPC2_peptide 4.876
IPC2.peptide.svr19 4.879
Protein with the highest isoelectric point:
>tr|A0A068J9V0|A0A068J9V0_9RHAB PAG3 protein OS=Curionopolis virus OX=490110 GN=pAG3 PE=4 SV=1
MM1 pKa = 7.5 IKK3 pKa = 9.63 IRR5 pKa = 11.84 KK6 pKa = 8.45 EE7 pKa = 4.01 GSSKK11 pKa = 11.3 DD12 pKa = 3.75 DD13 pKa = 3.33 FLFYY17 pKa = 10.81 QKK19 pKa = 10.6 VGEE22 pKa = 4.41 RR23 pKa = 11.84 IQNIFPDD30 pKa = 4.98 AINLKK35 pKa = 7.35 VHH37 pKa = 7.11 RR38 pKa = 11.84 SQDD41 pKa = 3.17 GVFILDD47 pKa = 3.8 LTWPPGVSVLLVPRR61 pKa = 11.84 RR62 pKa = 11.84 LKK64 pKa = 8.73 TVRR67 pKa = 11.84 RR68 pKa = 11.84 FVSEE72 pKa = 3.9 RR73 pKa = 11.84 PGRR76 pKa = 11.84 EE77 pKa = 3.69 LFLLGDD83 pKa = 3.96 LLYY86 pKa = 11.45 NKK88 pKa = 9.44 MGLKK92 pKa = 10.24 RR93 pKa = 11.84 SQIDD97 pKa = 3.1 TSYY100 pKa = 11.76 EE101 pKa = 3.97 MIHH104 pKa = 5.87 GGKK107 pKa = 8.19 WAIISIYY114 pKa = 10.4 GG115 pKa = 3.35
Molecular weight: 13.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.107
IPC2_protein 9.311
IPC_protein 9.385
Toseland 10.116
ProMoST 9.75
Dawson 10.277
Bjellqvist 9.926
Wikipedia 10.438
Rodwell 10.73
Grimsley 10.35
Solomon 10.321
Lehninger 10.292
Nozaki 10.072
DTASelect 9.926
Thurlkill 10.145
EMBOSS 10.511
Sillero 10.189
Patrickios 10.452
IPC_peptide 10.321
IPC2_peptide 8.404
IPC2.peptide.svr19 8.39
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
4138
68
2125
517.3
59.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.19 ± 0.476
1.957 ± 0.362
5.848 ± 0.453
8.482 ± 0.64
4.567 ± 0.299
6.525 ± 0.255
1.885 ± 0.119
7.129 ± 0.267
8.458 ± 0.455
9.884 ± 1.077
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.417 ± 0.362
4.132 ± 0.38
4.132 ± 0.436
2.658 ± 0.198
5.752 ± 0.653
8.627 ± 0.726
4.519 ± 0.271
4.833 ± 0.316
1.812 ± 0.165
3.19 ± 0.318
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here