Curionopolis virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Curiovirus; Curionopolis curiovirus

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A068J4Q8|A0A068J4Q8_9RHAB Nucleocapsid protein OS=Curionopolis virus OX=490110 GN=N PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 10.62SLGDD6 pKa = 3.44WLFQYY11 pKa = 11.16SLDD14 pKa = 3.28RR15 pKa = 11.84SLFVTRR21 pKa = 11.84MFEE24 pKa = 4.29SEE26 pKa = 4.39EE27 pKa = 4.11EE28 pKa = 4.03FLDD31 pKa = 4.75FYY33 pKa = 11.53VDD35 pKa = 5.04LYY37 pKa = 10.57IACKK41 pKa = 9.99SVNEE45 pKa = 4.57SIHH48 pKa = 4.58VTVTRR53 pKa = 11.84SLIDD57 pKa = 3.59PFHH60 pKa = 7.14VIFRR64 pKa = 11.84IWQTT68 pKa = 3.04

Molecular weight:
8.21 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A068J9V0|A0A068J9V0_9RHAB PAG3 protein OS=Curionopolis virus OX=490110 GN=pAG3 PE=4 SV=1
MM1 pKa = 7.5IKK3 pKa = 9.63IRR5 pKa = 11.84KK6 pKa = 8.45EE7 pKa = 4.01GSSKK11 pKa = 11.3DD12 pKa = 3.75DD13 pKa = 3.33FLFYY17 pKa = 10.81QKK19 pKa = 10.6VGEE22 pKa = 4.41RR23 pKa = 11.84IQNIFPDD30 pKa = 4.98AINLKK35 pKa = 7.35VHH37 pKa = 7.11RR38 pKa = 11.84SQDD41 pKa = 3.17GVFILDD47 pKa = 3.8LTWPPGVSVLLVPRR61 pKa = 11.84RR62 pKa = 11.84LKK64 pKa = 8.73TVRR67 pKa = 11.84RR68 pKa = 11.84FVSEE72 pKa = 3.9RR73 pKa = 11.84PGRR76 pKa = 11.84EE77 pKa = 3.69LFLLGDD83 pKa = 3.96LLYY86 pKa = 11.45NKK88 pKa = 9.44MGLKK92 pKa = 10.24RR93 pKa = 11.84SQIDD97 pKa = 3.1TSYY100 pKa = 11.76EE101 pKa = 3.97MIHH104 pKa = 5.87GGKK107 pKa = 8.19WAIISIYY114 pKa = 10.4GG115 pKa = 3.35

Molecular weight:
13.32 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

4138

68

2125

517.3

59.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.19 ± 0.476

1.957 ± 0.362

5.848 ± 0.453

8.482 ± 0.64

4.567 ± 0.299

6.525 ± 0.255

1.885 ± 0.119

7.129 ± 0.267

8.458 ± 0.455

9.884 ± 1.077

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.417 ± 0.362

4.132 ± 0.38

4.132 ± 0.436

2.658 ± 0.198

5.752 ± 0.653

8.627 ± 0.726

4.519 ± 0.271

4.833 ± 0.316

1.812 ± 0.165

3.19 ± 0.318

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski