Canarypox virus (CNPV)
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 322 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q6VZ64|Q6VZ64_CNPV CC chemokine-like protein OS=Canarypox virus OX=44088 GN=CNPV283 PE=4 SV=1
MM1 pKa = 7.27 NNNVKK6 pKa = 10.47 LMFMVFILILTKK18 pKa = 10.45 TIAQKK23 pKa = 10.03 MDD25 pKa = 3.0 APTTTSYY32 pKa = 7.91 TTEE35 pKa = 4.11 EE36 pKa = 4.2 EE37 pKa = 4.23 DD38 pKa = 4.99 DD39 pKa = 4.59 SEE41 pKa = 5.16 EE42 pKa = 4.21 EE43 pKa = 4.93 DD44 pKa = 4.28 DD45 pKa = 6.08 NEE47 pKa = 5.26 DD48 pKa = 4.46 EE49 pKa = 5.67 PEE51 pKa = 3.86 QDD53 pKa = 3.29 CCKK56 pKa = 10.98 DD57 pKa = 3.46 EE58 pKa = 4.36 TGCPYY63 pKa = 10.46 VSNSIYY69 pKa = 10.57 VSNGTTFNVVCVGCSSDD86 pKa = 3.66 YY87 pKa = 11.85 NNFTLLWMVNGTVIEE102 pKa = 4.4 NNTDD106 pKa = 2.79 TGFTYY111 pKa = 10.7 NISVLDD117 pKa = 3.68 VSSNFSSATVLSSVNLNRR135 pKa = 11.84 HH136 pKa = 6.6 DD137 pKa = 4.3 YY138 pKa = 9.84 NNSNITCILNDD149 pKa = 3.82 PKK151 pKa = 11.02 GSKK154 pKa = 9.91 SDD156 pKa = 3.67 SFDD159 pKa = 3.33 TKK161 pKa = 11.36 SMNNRR166 pKa = 11.84 YY167 pKa = 9.3 EE168 pKa = 4.12 KK169 pKa = 10.18 IRR171 pKa = 11.84 YY172 pKa = 8.78 LKK174 pKa = 10.35 EE175 pKa = 3.77 NNITLATEE183 pKa = 4.24 EE184 pKa = 4.43 EE185 pKa = 4.55 FNNYY189 pKa = 8.59 TITVKK194 pKa = 10.68 KK195 pKa = 10.64
Molecular weight: 22.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.901
IPC2_protein 4.177
IPC_protein 4.126
Toseland 3.935
ProMoST 4.253
Dawson 4.088
Bjellqvist 4.24
Wikipedia 3.973
Rodwell 3.948
Grimsley 3.846
Solomon 4.075
Lehninger 4.037
Nozaki 4.19
DTASelect 4.38
Thurlkill 3.961
EMBOSS 3.986
Sillero 4.228
Patrickios 1.952
IPC_peptide 4.075
IPC2_peptide 4.215
IPC2.peptide.svr19 4.131
Protein with the highest isoelectric point:
>tr|Q6VZJ4|Q6VZJ4_CNPV ATP-dependent helicase Rep OS=Canarypox virus OX=44088 GN=CNPV153 PE=3 SV=1
MM1 pKa = 7.34 MLNPPQVEE9 pKa = 4.15 ASAHH13 pKa = 4.88 CVRR16 pKa = 11.84 LLYY19 pKa = 10.42 ALKK22 pKa = 10.7 GSLLLDD28 pKa = 3.39 MAGNGILIALTFPIRR43 pKa = 11.84 KK44 pKa = 9.12 KK45 pKa = 11.02 VVFKK49 pKa = 11.07 HH50 pKa = 5.3 NNSYY54 pKa = 11.27 DD55 pKa = 3.97 SNPCSPSRR63 pKa = 11.84 RR64 pKa = 11.84 FKK66 pKa = 11.03 LRR68 pKa = 11.84 ALFLLSLLGLKK79 pKa = 10.39 LRR81 pKa = 11.84 DD82 pKa = 3.59 LYY84 pKa = 10.86 RR85 pKa = 11.84 KK86 pKa = 9.94 RR87 pKa = 11.84 FISIHH92 pKa = 5.09
Molecular weight: 10.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.328
IPC2_protein 9.853
IPC_protein 10.496
Toseland 10.774
ProMoST 10.409
Dawson 10.862
Bjellqvist 10.54
Wikipedia 11.052
Rodwell 11.228
Grimsley 10.906
Solomon 10.965
Lehninger 10.935
Nozaki 10.76
DTASelect 10.54
Thurlkill 10.76
EMBOSS 11.169
Sillero 10.789
Patrickios 10.979
IPC_peptide 10.965
IPC2_peptide 9.545
IPC2.peptide.svr19 8.458
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
322
0
322
106963
45
1951
332.2
38.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.485 ± 0.088
2.23 ± 0.071
6.439 ± 0.09
5.926 ± 0.146
3.958 ± 0.101
3.847 ± 0.106
1.983 ± 0.053
9.644 ± 0.161
8.379 ± 0.135
9.203 ± 0.157
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.571 ± 0.054
7.626 ± 0.149
2.93 ± 0.082
1.92 ± 0.087
4.137 ± 0.105
7.878 ± 0.168
5.506 ± 0.107
5.957 ± 0.083
0.658 ± 0.036
5.722 ± 0.105
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here