Amino acid dipepetide frequency for Ruminococcus sp. YE71

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.594AlaAla: 9.594 ± 0.148
1.165AlaCys: 1.165 ± 0.033
6.14AlaAsp: 6.14 ± 0.071
7.318AlaGlu: 7.318 ± 0.109
3.261AlaPhe: 3.261 ± 0.072
5.338AlaGly: 5.338 ± 0.085
1.241AlaHis: 1.241 ± 0.039
4.208AlaIle: 4.208 ± 0.065
4.87AlaLys: 4.87 ± 0.075
6.897AlaLeu: 6.897 ± 0.079
2.163AlaMet: 2.163 ± 0.05
2.686AlaAsn: 2.686 ± 0.049
2.373AlaPro: 2.373 ± 0.049
2.256AlaGln: 2.256 ± 0.046
2.861AlaArg: 2.861 ± 0.051
4.335AlaSer: 4.335 ± 0.072
2.873AlaThr: 2.873 ± 0.057
7.79AlaVal: 7.79 ± 0.101
0.588AlaTrp: 0.588 ± 0.022
2.795AlaTyr: 2.795 ± 0.049
0.0AlaXaa: 0.0 ± 0.0
Cys
1.458CysAla: 1.458 ± 0.043
0.345CysCys: 0.345 ± 0.02
1.089CysAsp: 1.089 ± 0.033
1.224CysGlu: 1.224 ± 0.033
0.714CysPhe: 0.714 ± 0.026
1.904CysGly: 1.904 ± 0.04
0.272CysHis: 0.272 ± 0.017
1.089CysIle: 1.089 ± 0.034
0.895CysLys: 0.895 ± 0.032
1.112CysLeu: 1.112 ± 0.031
0.394CysMet: 0.394 ± 0.018
0.495CysAsn: 0.495 ± 0.02
0.684CysPro: 0.684 ± 0.025
0.28CysGln: 0.28 ± 0.016
0.87CysArg: 0.87 ± 0.028
1.141CysSer: 1.141 ± 0.032
1.04CysThr: 1.04 ± 0.033
1.105CysVal: 1.105 ± 0.031
0.145CysTrp: 0.145 ± 0.012
0.629CysTyr: 0.629 ± 0.025
0.0CysXaa: 0.0 ± 0.0
Asp
4.131AspAla: 4.131 ± 0.064
1.045AspCys: 1.045 ± 0.032
4.107AspAsp: 4.107 ± 0.072
5.498AspGlu: 5.498 ± 0.075
2.869AspPhe: 2.869 ± 0.049
5.432AspGly: 5.432 ± 0.09
0.967AspHis: 0.967 ± 0.033
4.769AspIle: 4.769 ± 0.067
4.306AspLys: 4.306 ± 0.073
4.668AspLeu: 4.668 ± 0.067
1.927AspMet: 1.927 ± 0.043
2.671AspAsn: 2.671 ± 0.059
2.337AspPro: 2.337 ± 0.05
1.012AspGln: 1.012 ± 0.031
3.026AspArg: 3.026 ± 0.056
4.441AspSer: 4.441 ± 0.087
3.778AspThr: 3.778 ± 0.051
3.721AspVal: 3.721 ± 0.062
0.623AspTrp: 0.623 ± 0.024
2.866AspTyr: 2.866 ± 0.055
0.0AspXaa: 0.0 ± 0.0
Glu
4.689GluAla: 4.689 ± 0.068
0.99GluCys: 0.99 ± 0.028
3.897GluAsp: 3.897 ± 0.07
5.19GluGlu: 5.19 ± 0.093
2.734GluPhe: 2.734 ± 0.046
4.355GluGly: 4.355 ± 0.058
1.229GluHis: 1.229 ± 0.037
4.977GluIle: 4.977 ± 0.076
5.686GluLys: 5.686 ± 0.069
7.124GluLeu: 7.124 ± 0.092
2.237GluMet: 2.237 ± 0.046
3.851GluAsn: 3.851 ± 0.058
2.141GluPro: 2.141 ± 0.051
2.85GluGln: 2.85 ± 0.055
3.8GluArg: 3.8 ± 0.076
3.848GluSer: 3.848 ± 0.067
4.081GluThr: 4.081 ± 0.065
3.929GluVal: 3.929 ± 0.071
0.776GluTrp: 0.776 ± 0.026
3.172GluTyr: 3.172 ± 0.059
0.001GluXaa: 0.001 ± 0.001
Phe
3.474PheAla: 3.474 ± 0.061
0.87PheCys: 0.87 ± 0.027
3.102PheAsp: 3.102 ± 0.055
3.032PheGlu: 3.032 ± 0.056
1.883PhePhe: 1.883 ± 0.052
3.627PheGly: 3.627 ± 0.056
0.621PheHis: 0.621 ± 0.02
2.818PheIle: 2.818 ± 0.056
2.07PheLys: 2.07 ± 0.042
3.064PheLeu: 3.064 ± 0.054
1.161PheMet: 1.161 ± 0.037
1.594PheAsn: 1.594 ± 0.038
1.295PhePro: 1.295 ± 0.034
0.764PheGln: 0.764 ± 0.03
1.986PheArg: 1.986 ± 0.049
3.086PheSer: 3.086 ± 0.054
2.879PheThr: 2.879 ± 0.049
2.799PheVal: 2.799 ± 0.055
0.371PheTrp: 0.371 ± 0.018
1.687PheTyr: 1.687 ± 0.035
0.0PheXaa: 0.0 ± 0.0
Gly
5.201GlyAla: 5.201 ± 0.08
1.501GlyCys: 1.501 ± 0.042
4.185GlyAsp: 4.185 ± 0.074
5.052GlyGlu: 5.052 ± 0.068
3.315GlyPhe: 3.315 ± 0.064
5.455GlyGly: 5.455 ± 0.101
1.256GlyHis: 1.256 ± 0.031
5.263GlyIle: 5.263 ± 0.077
5.284GlyLys: 5.284 ± 0.081
5.784GlyLeu: 5.784 ± 0.083
2.239GlyMet: 2.239 ± 0.044
2.754GlyAsn: 2.754 ± 0.056
0.835GlyPro: 0.835 ± 0.029
1.815GlyGln: 1.815 ± 0.038
3.449GlyArg: 3.449 ± 0.054
5.001GlySer: 5.001 ± 0.077
4.429GlyThr: 4.429 ± 0.088
4.947GlyVal: 4.947 ± 0.069
0.756GlyTrp: 0.756 ± 0.031
3.418GlyTyr: 3.418 ± 0.061
0.0GlyXaa: 0.0 ± 0.0
His
0.998HisAla: 0.998 ± 0.03
0.311HisCys: 0.311 ± 0.015
1.111HisAsp: 1.111 ± 0.034
1.119HisGlu: 1.119 ± 0.031
0.827HisPhe: 0.827 ± 0.028
1.279HisGly: 1.279 ± 0.038
0.375HisHis: 0.375 ± 0.025
1.33HisIle: 1.33 ± 0.04
0.923HisLys: 0.923 ± 0.028
1.222HisLeu: 1.222 ± 0.032
0.422HisMet: 0.422 ± 0.02
0.719HisAsn: 0.719 ± 0.025
0.675HisPro: 0.675 ± 0.026
0.367HisGln: 0.367 ± 0.018
0.86HisArg: 0.86 ± 0.025
1.146HisSer: 1.146 ± 0.033
0.952HisThr: 0.952 ± 0.027
0.771HisVal: 0.771 ± 0.026
0.139HisTrp: 0.139 ± 0.011
0.705HisTyr: 0.705 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
5.773IleAla: 5.773 ± 0.074
1.329IleCys: 1.329 ± 0.036
4.472IleAsp: 4.472 ± 0.071
4.534IleGlu: 4.534 ± 0.06
2.637IlePhe: 2.637 ± 0.057
4.899IleGly: 4.899 ± 0.067
1.077IleHis: 1.077 ± 0.03
4.713IleIle: 4.713 ± 0.076
3.951IleLys: 3.951 ± 0.062
5.166IleLeu: 5.166 ± 0.072
1.809IleMet: 1.809 ± 0.044
2.923IleAsn: 2.923 ± 0.053
2.871IlePro: 2.871 ± 0.056
1.413IleGln: 1.413 ± 0.041
3.343IleArg: 3.343 ± 0.06
4.978IleSer: 4.978 ± 0.066
4.458IleThr: 4.458 ± 0.072
4.514IleVal: 4.514 ± 0.062
0.511IleTrp: 0.511 ± 0.021
2.48IleTyr: 2.48 ± 0.045
0.0IleXaa: 0.0 ± 0.0
Lys
5.155LysAla: 5.155 ± 0.068
0.841LysCys: 0.841 ± 0.026
3.621LysAsp: 3.621 ± 0.062
4.228LysGlu: 4.228 ± 0.067
1.956LysPhe: 1.956 ± 0.043
4.02LysGly: 4.02 ± 0.056
1.099LysHis: 1.099 ± 0.031
4.047LysIle: 4.047 ± 0.057
5.209LysLys: 5.209 ± 0.075
5.999LysLeu: 5.999 ± 0.068
1.863LysMet: 1.863 ± 0.037
3.413LysAsn: 3.413 ± 0.062
2.213LysPro: 2.213 ± 0.05
2.008LysGln: 2.008 ± 0.05
3.232LysArg: 3.232 ± 0.054
3.622LysSer: 3.622 ± 0.057
3.954LysThr: 3.954 ± 0.063
3.759LysVal: 3.759 ± 0.055
0.658LysTrp: 0.658 ± 0.026
2.924LysTyr: 2.924 ± 0.053
0.0LysXaa: 0.0 ± 0.0
Leu
6.771LeuAla: 6.771 ± 0.094
1.613LeuCys: 1.613 ± 0.044
5.231LeuAsp: 5.231 ± 0.074
5.131LeuGlu: 5.131 ± 0.074
3.627LeuPhe: 3.627 ± 0.065
5.823LeuGly: 5.823 ± 0.075
1.361LeuHis: 1.361 ± 0.036
5.682LeuIle: 5.682 ± 0.091
5.167LeuLys: 5.167 ± 0.069
7.384LeuLeu: 7.384 ± 0.105
2.501LeuMet: 2.501 ± 0.043
3.41LeuAsn: 3.41 ± 0.058
3.434LeuPro: 3.434 ± 0.063
1.708LeuGln: 1.708 ± 0.039
4.189LeuArg: 4.189 ± 0.068
6.392LeuSer: 6.392 ± 0.08
6.026LeuThr: 6.026 ± 0.073
4.804LeuVal: 4.804 ± 0.064
0.694LeuTrp: 0.694 ± 0.028
3.155LeuTyr: 3.155 ± 0.058
0.0LeuXaa: 0.0 ± 0.0
Met
2.072MetAla: 2.072 ± 0.046
0.432MetCys: 0.432 ± 0.018
1.315MetAsp: 1.315 ± 0.034
1.542MetGlu: 1.542 ± 0.042
1.1MetPhe: 1.1 ± 0.031
1.671MetGly: 1.671 ± 0.041
0.408MetHis: 0.408 ± 0.017
1.975MetIle: 1.975 ± 0.044
2.189MetLys: 2.189 ± 0.047
2.948MetLeu: 2.948 ± 0.056
0.845MetMet: 0.845 ± 0.029
1.437MetAsn: 1.437 ± 0.034
1.166MetPro: 1.166 ± 0.029
0.879MetGln: 0.879 ± 0.027
1.377MetArg: 1.377 ± 0.036
1.981MetSer: 1.981 ± 0.045
2.029MetThr: 2.029 ± 0.049
1.469MetVal: 1.469 ± 0.038
0.225MetTrp: 0.225 ± 0.014
0.984MetTyr: 0.984 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
3.554AsnAla: 3.554 ± 0.069
0.745AsnCys: 0.745 ± 0.03
2.716AsnAsp: 2.716 ± 0.056
2.841AsnGlu: 2.841 ± 0.054
1.559AsnPhe: 1.559 ± 0.038
4.007AsnGly: 4.007 ± 0.068
0.558AsnHis: 0.558 ± 0.021
3.409AsnIle: 3.409 ± 0.06
2.469AsnLys: 2.469 ± 0.055
2.724AsnLeu: 2.724 ± 0.057
1.113AsnMet: 1.113 ± 0.03
2.003AsnAsn: 2.003 ± 0.057
1.607AsnPro: 1.607 ± 0.034
0.819AsnGln: 0.819 ± 0.029
1.803AsnArg: 1.803 ± 0.043
2.818AsnSer: 2.818 ± 0.055
2.511AsnThr: 2.511 ± 0.053
2.862AsnVal: 2.862 ± 0.051
0.399AsnTrp: 0.399 ± 0.022
1.848AsnTyr: 1.848 ± 0.044
0.0AsnXaa: 0.0 ± 0.0
Pro
2.571ProAla: 2.571 ± 0.048
0.523ProCys: 0.523 ± 0.023
3.161ProAsp: 3.161 ± 0.058
3.347ProGlu: 3.347 ± 0.06
1.554ProPhe: 1.554 ± 0.04
1.419ProGly: 1.419 ± 0.037
0.578ProHis: 0.578 ± 0.023
1.933ProIle: 1.933 ± 0.04
2.09ProLys: 2.09 ± 0.048
2.539ProLeu: 2.539 ± 0.047
0.918ProMet: 0.918 ± 0.031
1.402ProAsn: 1.402 ± 0.039
1.025ProPro: 1.025 ± 0.038
1.124ProGln: 1.124 ± 0.041
1.042ProArg: 1.042 ± 0.035
2.009ProSer: 2.009 ± 0.046
1.835ProThr: 1.835 ± 0.044
2.799ProVal: 2.799 ± 0.055
0.252ProTrp: 0.252 ± 0.016
1.359ProTyr: 1.359 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
1.95GlnAla: 1.95 ± 0.039
0.33GlnCys: 0.33 ± 0.019
1.105GlnAsp: 1.105 ± 0.03
1.582GlnGlu: 1.582 ± 0.036
1.036GlnPhe: 1.036 ± 0.031
1.654GlnGly: 1.654 ± 0.036
0.431GlnHis: 0.431 ± 0.021
1.859GlnIle: 1.859 ± 0.043
1.875GlnLys: 1.875 ± 0.04
2.758GlnLeu: 2.758 ± 0.055
0.8GlnMet: 0.8 ± 0.029
1.276GlnAsn: 1.276 ± 0.032
0.981GlnPro: 0.981 ± 0.032
1.108GlnGln: 1.108 ± 0.043
1.331GlnArg: 1.331 ± 0.041
1.528GlnSer: 1.528 ± 0.039
1.673GlnThr: 1.673 ± 0.047
1.402GlnVal: 1.402 ± 0.031
0.234GlnTrp: 0.234 ± 0.016
1.176GlnTyr: 1.176 ± 0.033
0.0GlnXaa: 0.0 ± 0.0
Arg
3.172ArgAla: 3.172 ± 0.052
0.706ArgCys: 0.706 ± 0.022
2.57ArgAsp: 2.57 ± 0.046
3.611ArgGlu: 3.611 ± 0.061
2.235ArgPhe: 2.235 ± 0.051
2.577ArgGly: 2.577 ± 0.055
0.894ArgHis: 0.894 ± 0.029
3.498ArgIle: 3.498 ± 0.052
3.019ArgLys: 3.019 ± 0.059
4.488ArgLeu: 4.488 ± 0.073
1.412ArgMet: 1.412 ± 0.038
1.88ArgAsn: 1.88 ± 0.039
1.463ArgPro: 1.463 ± 0.038
1.53ArgGln: 1.53 ± 0.045
2.777ArgArg: 2.777 ± 0.065
2.923ArgSer: 2.923 ± 0.056
2.646ArgThr: 2.646 ± 0.048
2.788ArgVal: 2.788 ± 0.051
0.418ArgTrp: 0.418 ± 0.017
1.985ArgTyr: 1.985 ± 0.041
0.001ArgXaa: 0.001 ± 0.001
Ser
6.181SerAla: 6.181 ± 0.088
1.009SerCys: 1.009 ± 0.028
4.705SerAsp: 4.705 ± 0.071
5.062SerGlu: 5.062 ± 0.079
3.001SerPhe: 3.001 ± 0.052
6.064SerGly: 6.064 ± 0.105
0.967SerHis: 0.967 ± 0.028
3.886SerIle: 3.886 ± 0.059
3.543SerLys: 3.543 ± 0.058
5.129SerLeu: 5.129 ± 0.076
1.642SerMet: 1.642 ± 0.036
2.31SerAsn: 2.31 ± 0.05
2.011SerPro: 2.011 ± 0.044
1.708SerGln: 1.708 ± 0.036
2.838SerArg: 2.838 ± 0.062
4.753SerSer: 4.753 ± 0.124
3.31SerThr: 3.31 ± 0.079
5.024SerVal: 5.024 ± 0.07
0.628SerTrp: 0.628 ± 0.028
2.619SerTyr: 2.619 ± 0.058
0.0SerXaa: 0.0 ± 0.0
Thr
6.392ThrAla: 6.392 ± 0.091
0.765ThrCys: 0.765 ± 0.028
4.491ThrAsp: 4.491 ± 0.074
4.387ThrGlu: 4.387 ± 0.069
2.357ThrPhe: 2.357 ± 0.049
4.538ThrGly: 4.538 ± 0.077
0.933ThrHis: 0.933 ± 0.025
3.819ThrIle: 3.819 ± 0.076
3.099ThrLys: 3.099 ± 0.062
5.09ThrLeu: 5.09 ± 0.074
1.302ThrMet: 1.302 ± 0.032
2.102ThrAsn: 2.102 ± 0.047
2.31ThrPro: 2.31 ± 0.046
1.419ThrGln: 1.419 ± 0.034
1.962ThrArg: 1.962 ± 0.039
3.636ThrSer: 3.636 ± 0.074
3.243ThrThr: 3.243 ± 0.095
5.439ThrVal: 5.439 ± 0.079
0.47ThrTrp: 0.47 ± 0.023
2.251ThrTyr: 2.251 ± 0.048
0.0ThrXaa: 0.0 ± 0.0
Val
4.116ValAla: 4.116 ± 0.069
1.419ValCys: 1.419 ± 0.036
3.518ValAsp: 3.518 ± 0.062
3.613ValGlu: 3.613 ± 0.059
3.205ValPhe: 3.205 ± 0.057
4.252ValGly: 4.252 ± 0.064
1.025ValHis: 1.025 ± 0.03
5.233ValIle: 5.233 ± 0.069
4.283ValLys: 4.283 ± 0.065
5.863ValLeu: 5.863 ± 0.07
2.082ValMet: 2.082 ± 0.057
2.989ValAsn: 2.989 ± 0.05
2.545ValPro: 2.545 ± 0.045
1.661ValGln: 1.661 ± 0.039
3.401ValArg: 3.401 ± 0.063
5.178ValSer: 5.178 ± 0.07
5.078ValThr: 5.078 ± 0.098
4.19ValVal: 4.19 ± 0.067
0.649ValTrp: 0.649 ± 0.023
2.818ValTyr: 2.818 ± 0.062
0.0ValXaa: 0.0 ± 0.0
Trp
0.622TrpAla: 0.622 ± 0.024
0.183TrpCys: 0.183 ± 0.011
0.662TrpAsp: 0.662 ± 0.029
0.578TrpGlu: 0.578 ± 0.024
0.408TrpPhe: 0.408 ± 0.021
0.659TrpGly: 0.659 ± 0.028
0.224TrpHis: 0.224 ± 0.014
0.49TrpIle: 0.49 ± 0.023
0.558TrpLys: 0.558 ± 0.022
0.861TrpLeu: 0.861 ± 0.029
0.219TrpMet: 0.219 ± 0.013
0.525TrpAsn: 0.525 ± 0.027
0.08TrpPro: 0.08 ± 0.008
0.344TrpGln: 0.344 ± 0.017
0.375TrpArg: 0.375 ± 0.017
0.581TrpSer: 0.581 ± 0.024
0.522TrpThr: 0.522 ± 0.029
0.548TrpVal: 0.548 ± 0.022
0.115TrpTrp: 0.115 ± 0.012
0.466TrpTyr: 0.466 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.879TyrAla: 2.879 ± 0.051
0.764TyrCys: 0.764 ± 0.026
2.997TyrAsp: 2.997 ± 0.057
2.669TyrGlu: 2.669 ± 0.051
1.906TyrPhe: 1.906 ± 0.047
2.964TyrGly: 2.964 ± 0.057
0.735TyrHis: 0.735 ± 0.024
2.852TyrIle: 2.852 ± 0.048
2.335TyrLys: 2.335 ± 0.048
3.283TyrLeu: 3.283 ± 0.06
0.994TyrMet: 0.994 ± 0.028
1.925TyrAsn: 1.925 ± 0.048
1.436TyrPro: 1.436 ± 0.037
1.021TyrGln: 1.021 ± 0.026
2.071TyrArg: 2.071 ± 0.047
2.995TyrSer: 2.995 ± 0.058
2.687TyrThr: 2.687 ± 0.067
2.46TyrVal: 2.46 ± 0.048
0.365TyrTrp: 0.365 ± 0.021
2.003TyrTyr: 2.003 ± 0.053
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.001XaaLeu: 0.001 ± 0.001
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.001XaaThr: 0.001 ± 0.001
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.014XaaXaa: 0.014 ± 0.013
Statistics based on 3572 proteins (1169459 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski