Mariniphaga anaerophila

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Marinilabiliales; Prolixibacteraceae; Mariniphaga

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3974 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M5ETJ0|A0A1M5ETJ0_9BACT Uncharacterized protein OS=Mariniphaga anaerophila OX=1484053 GN=SAMN05444274_11021 PE=4 SV=1
MM1 pKa = 7.41KK2 pKa = 10.24KK3 pKa = 10.3FYY5 pKa = 10.51FFSFFALLFFIGSGLTFAAEE25 pKa = 3.69IGDD28 pKa = 4.0NIKK31 pKa = 10.39IGSNQQSAKK40 pKa = 10.12FPQLTYY46 pKa = 10.63CSEE49 pKa = 3.78QDD51 pKa = 3.59KK52 pKa = 11.26ILMVWNGNDD61 pKa = 3.28SGTDD65 pKa = 3.12QIFFKK70 pKa = 10.71FLTHH74 pKa = 6.8SEE76 pKa = 4.32VSQNPSAPGMQLSSGSSPWNRR97 pKa = 11.84YY98 pKa = 6.9SEE100 pKa = 4.47RR101 pKa = 11.84PRR103 pKa = 11.84AAYY106 pKa = 9.71DD107 pKa = 3.44AFSATAAVVWQEE119 pKa = 3.99SNAVGKK125 pKa = 10.35DD126 pKa = 3.55SVVMAILDD134 pKa = 3.74VNTEE138 pKa = 4.03SKK140 pKa = 9.63LTEE143 pKa = 3.95VVVAANSSWNMTATVASDD161 pKa = 3.5NNGSFLVAYY170 pKa = 9.75YY171 pKa = 10.79DD172 pKa = 3.71SASTNITAKK181 pKa = 10.83LFDD184 pKa = 4.44ANGAQSGSDD193 pKa = 3.6VILGTVAGGYY203 pKa = 7.24MFPYY207 pKa = 10.23SIDD210 pKa = 2.95IAYY213 pKa = 10.52NSEE216 pKa = 3.91NEE218 pKa = 3.99IFLVTWADD226 pKa = 3.58YY227 pKa = 11.18NSNLFSRR234 pKa = 11.84TLNSDD239 pKa = 3.4GTAGEE244 pKa = 4.24LNVYY248 pKa = 10.7NSIQGVANPSVAYY261 pKa = 10.19NDD263 pKa = 3.67SLNIFLIAYY272 pKa = 9.72DD273 pKa = 4.01DD274 pKa = 3.85FAGNVSAQLTDD285 pKa = 3.12SLGILSGSLFTFGSSSALEE304 pKa = 4.28AEE306 pKa = 4.28PDD308 pKa = 3.27VAYY311 pKa = 10.46NAKK314 pKa = 10.45NNGFAVTWSDD324 pKa = 3.61YY325 pKa = 11.32LGGSGVWVQEE335 pKa = 4.1ILVSSCTVLVDD346 pKa = 3.68SVKK349 pKa = 9.75MDD351 pKa = 4.41KK352 pKa = 10.59ISATTNSSSVAYY364 pKa = 10.15DD365 pKa = 3.6AVNDD369 pKa = 4.28SYY371 pKa = 10.94WVAWYY376 pKa = 10.5GEE378 pKa = 4.23SGGAKK383 pKa = 10.21DD384 pKa = 3.98EE385 pKa = 5.08LFLQRR390 pKa = 11.84YY391 pKa = 7.72RR392 pKa = 11.84SEE394 pKa = 4.47NISIKK399 pKa = 10.71ANCKK403 pKa = 10.47DD404 pKa = 2.77ITVYY408 pKa = 10.88LDD410 pKa = 3.31EE411 pKa = 5.31NGEE414 pKa = 4.38YY415 pKa = 10.47VLSAEE420 pKa = 4.65DD421 pKa = 3.43VDD423 pKa = 4.36NGSFLLCGHH432 pKa = 5.89VQMDD436 pKa = 3.46IDD438 pKa = 3.64VDD440 pKa = 3.89EE441 pKa = 5.33ALTCAVTQPVEE452 pKa = 4.1VRR454 pKa = 11.84LTASDD459 pKa = 3.81GDD461 pKa = 4.05EE462 pKa = 3.95QTDD465 pKa = 3.97YY466 pKa = 11.44CVATVTVLDD475 pKa = 4.08TVPPVPVCEE484 pKa = 4.18NKK486 pKa = 10.33TIFLDD491 pKa = 4.12ANGEE495 pKa = 4.16AYY497 pKa = 7.59LTPEE501 pKa = 5.32DD502 pKa = 4.12IDD504 pKa = 4.56GGSTDD509 pKa = 3.01NCGIQAIWLDD519 pKa = 3.32KK520 pKa = 10.6TDD522 pKa = 5.54FYY524 pKa = 11.25TADD527 pKa = 3.52LGEE530 pKa = 4.28NTVLLTVKK538 pKa = 10.43DD539 pKa = 3.54NAGIEE544 pKa = 4.24RR545 pKa = 11.84SCEE548 pKa = 3.94AIVTVSEE555 pKa = 5.37FIDD558 pKa = 3.71DD559 pKa = 3.96TPPSVRR565 pKa = 11.84TTPLTIYY572 pKa = 10.66LDD574 pKa = 3.83EE575 pKa = 4.91TGTASIEE582 pKa = 4.05PSDD585 pKa = 3.79IDD587 pKa = 4.31NGSFDD592 pKa = 3.98DD593 pKa = 5.7GEE595 pKa = 4.37IEE597 pKa = 4.92SLSLDD602 pKa = 3.44KK603 pKa = 11.31SVFTCDD609 pKa = 3.39DD610 pKa = 3.58ANRR613 pKa = 11.84TDD615 pKa = 3.4TMFALAPEE623 pKa = 4.62ASTGSVARR631 pKa = 11.84FVGSQGKK638 pKa = 8.42DD639 pKa = 2.56WKK641 pKa = 10.19YY642 pKa = 11.2DD643 pKa = 3.31GVAGSTGLGFNYY655 pKa = 10.0GFEE658 pKa = 4.68RR659 pKa = 11.84NLSDD663 pKa = 3.16GQYY666 pKa = 10.32YY667 pKa = 10.77LIGGASEE674 pKa = 4.71DD675 pKa = 3.53EE676 pKa = 4.28RR677 pKa = 11.84NLYY680 pKa = 10.4RR681 pKa = 11.84LDD683 pKa = 4.12AEE685 pKa = 4.47TFSAEE690 pKa = 4.4FITEE694 pKa = 4.33LMSSDD699 pKa = 3.57GDD701 pKa = 3.93EE702 pKa = 4.49EE703 pKa = 4.39PLDD706 pKa = 3.79IAIDD710 pKa = 3.55NNGYY714 pKa = 9.85FFIVYY719 pKa = 10.34KK720 pKa = 10.84NGTIDD725 pKa = 4.16KK726 pKa = 9.39LDD728 pKa = 3.99PVTWTAVEE736 pKa = 4.08HH737 pKa = 6.31ANIGAGLGEE746 pKa = 4.33VSLTYY751 pKa = 10.83DD752 pKa = 3.49NEE754 pKa = 4.07NEE756 pKa = 3.92RR757 pKa = 11.84LLFVHH762 pKa = 6.93FLQNEE767 pKa = 4.19AVNAPNVLSEE777 pKa = 3.92ISSNGTVTEE786 pKa = 4.28LFSFFTPGNDD796 pKa = 3.63SSCCALGIEE805 pKa = 4.47YY806 pKa = 10.2AGNGFCFASSAFNCDD821 pKa = 2.95ALFEE825 pKa = 5.19IDD827 pKa = 5.34LNNEE831 pKa = 4.12SVNLIAQPNGSSFSGLKK848 pKa = 10.34DD849 pKa = 4.43LIYY852 pKa = 10.47IPANMVTLIVTDD864 pKa = 3.84IYY866 pKa = 11.48GNSSEE871 pKa = 4.67GKK873 pKa = 9.08VKK875 pKa = 11.01VEE877 pKa = 3.78VVDD880 pKa = 4.5NIEE883 pKa = 4.02PALVLNQVQVYY894 pKa = 9.18LGPDD898 pKa = 3.24GTYY901 pKa = 10.48KK902 pKa = 9.37ITEE905 pKa = 3.86QDD907 pKa = 3.32IEE909 pKa = 4.36AMIAGTDD916 pKa = 3.99DD917 pKa = 3.65NCSSSGNLEE926 pKa = 3.8FEE928 pKa = 4.67VYY930 pKa = 10.2PKK932 pKa = 10.46IFDD935 pKa = 3.63CTMDD939 pKa = 4.1EE940 pKa = 4.2IVHH943 pKa = 5.28VRR945 pKa = 11.84VTAADD950 pKa = 3.14ASGNYY955 pKa = 7.26TRR957 pKa = 11.84AWTTVLVFDD966 pKa = 4.05TLSPVFEE973 pKa = 4.25PVADD977 pKa = 4.45FEE979 pKa = 5.13TTVSPGTSEE988 pKa = 4.13TPIEE992 pKa = 4.27YY993 pKa = 10.04PEE995 pKa = 4.1VLATDD1000 pKa = 3.59NCSVEE1005 pKa = 4.13VNLIGGLGPNGLFPVGVTTEE1025 pKa = 3.47KK1026 pKa = 10.17WVAVDD1031 pKa = 4.97DD1032 pKa = 4.67RR1033 pKa = 11.84GNSDD1037 pKa = 3.04TLMFNVTVVALNANPTINPISDD1059 pKa = 3.74VEE1061 pKa = 4.41VSEE1064 pKa = 4.37STSPITVEE1072 pKa = 4.12LAGISYY1078 pKa = 10.98GNDD1081 pKa = 2.72LVEE1084 pKa = 4.08QTVAVTAKK1092 pKa = 10.33ADD1094 pKa = 3.66NVDD1097 pKa = 3.83LVEE1100 pKa = 4.94SIFVNYY1106 pKa = 10.02EE1107 pKa = 3.8SGSTGSLEE1115 pKa = 3.89IEE1117 pKa = 4.18IASEE1121 pKa = 3.95RR1122 pKa = 11.84SGVAIITVNVIDD1134 pKa = 4.05SEE1136 pKa = 4.31GGEE1139 pKa = 4.0VSEE1142 pKa = 4.42TFRR1145 pKa = 11.84LTVIPVNKK1153 pKa = 9.68PPFLVYY1159 pKa = 10.07PISDD1163 pKa = 3.01QWVNASYY1170 pKa = 10.73VLKK1173 pKa = 11.0VPVSPVLGEE1182 pKa = 4.72LFDD1185 pKa = 5.46DD1186 pKa = 4.65PEE1188 pKa = 6.41GDD1190 pKa = 3.78ALTISAMLADD1200 pKa = 4.23GTVLPGWAEE1209 pKa = 3.93LVNDD1213 pKa = 3.92SLVFNPMIEE1222 pKa = 4.18NVGCIDD1228 pKa = 3.93VVVKK1232 pKa = 9.99ATDD1235 pKa = 3.35IEE1237 pKa = 4.78GGSATDD1243 pKa = 3.45TFAVCVEE1250 pKa = 4.74GYY1252 pKa = 9.27PVNADD1257 pKa = 3.88AISAGGLSVNLYY1269 pKa = 9.44PNPAVDD1275 pKa = 4.6KK1276 pKa = 10.72VNISIKK1282 pKa = 10.34SSSFEE1287 pKa = 3.94PTVVTVYY1294 pKa = 10.33TITGEE1299 pKa = 4.11LVLQGKK1305 pKa = 9.44FDD1307 pKa = 4.07AGQLISLDD1315 pKa = 3.66MAEE1318 pKa = 4.79HH1319 pKa = 5.78VSGVYY1324 pKa = 8.9FVKK1327 pKa = 9.97IDD1329 pKa = 3.42QNNRR1333 pKa = 11.84TVVKK1337 pKa = 10.59KK1338 pKa = 10.62LVLKK1342 pKa = 10.93GKK1344 pKa = 10.05

Molecular weight: 144.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M5DP20|A0A1M5DP20_9BACT SPFH domain / Band 7 family protein OS=Mariniphaga anaerophila OX=1484053 GN=SAMN05444274_107184 PE=4 SV=1
MM1 pKa = 7.66ISNIQKK7 pKa = 10.23NLIRR11 pKa = 11.84TLTLQKK17 pKa = 10.01RR18 pKa = 11.84AKK20 pKa = 9.3KK21 pKa = 9.65RR22 pKa = 11.84RR23 pKa = 11.84LIGRR27 pKa = 11.84LFSQKK32 pKa = 10.51KK33 pKa = 9.38FLFNLKK39 pKa = 10.07SSPHH43 pKa = 5.05TLSLRR48 pKa = 11.84FQQHH52 pKa = 4.79RR53 pKa = 11.84RR54 pKa = 11.84RR55 pKa = 11.84GQNPIFFGLIAQMDD69 pKa = 3.76

Molecular weight: 8.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3974

0

3974

1523357

26

4578

383.3

43.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.623 ± 0.036

0.835 ± 0.016

5.36 ± 0.031

6.846 ± 0.034

5.292 ± 0.031

7.021 ± 0.036

1.76 ± 0.017

7.028 ± 0.03

6.954 ± 0.043

9.015 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.287 ± 0.022

5.755 ± 0.037

3.836 ± 0.023

3.373 ± 0.02

4.073 ± 0.026

6.484 ± 0.032

5.407 ± 0.045

6.587 ± 0.032

1.383 ± 0.016

4.083 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski