Faecalibacterium sp. An121

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Faecalibacterium; unclassified Faecalibacterium

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2414 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y4TID2|A0A1Y4TID2_9FIRM Transcriptional regulator OS=Faecalibacterium sp. An121 OX=1965550 GN=B5E66_01150 PE=4 SV=1
MM1 pKa = 7.88RR2 pKa = 11.84KK3 pKa = 9.39LKK5 pKa = 10.65KK6 pKa = 10.14AVSLMMTAAMCLSFAACGGSASSASSASSAAASSGAASQEE46 pKa = 4.03AASAASSEE54 pKa = 4.33AEE56 pKa = 3.67SDD58 pKa = 3.42LAYY61 pKa = 10.99VQDD64 pKa = 3.79KK65 pKa = 8.67GTLVVGMTDD74 pKa = 4.33FAPMDD79 pKa = 3.56YY80 pKa = 10.09RR81 pKa = 11.84DD82 pKa = 4.95EE83 pKa = 4.35NGEE86 pKa = 4.21WIGFDD91 pKa = 3.61ADD93 pKa = 3.48MAKK96 pKa = 10.73AFAEE100 pKa = 4.09YY101 pKa = 10.48LGVDD105 pKa = 3.7VEE107 pKa = 4.37FLEE110 pKa = 5.01INWDD114 pKa = 3.69NKK116 pKa = 10.52LMEE119 pKa = 5.8LDD121 pKa = 3.72TKK123 pKa = 11.21GVDD126 pKa = 4.09AIWNGMTITDD136 pKa = 4.18EE137 pKa = 4.38VTNGASVSQPYY148 pKa = 9.84CNNGQVVVVPAEE160 pKa = 3.91KK161 pKa = 10.43AADD164 pKa = 3.81YY165 pKa = 7.76QTTDD169 pKa = 3.4SLSDD173 pKa = 3.26LAFAVEE179 pKa = 4.21NGSAGAEE186 pKa = 3.89QLDD189 pKa = 4.01ALGIPYY195 pKa = 9.44VAKK198 pKa = 8.33TTQADD203 pKa = 3.36ALMEE207 pKa = 4.33VASGASDD214 pKa = 3.3ACVIDD219 pKa = 4.81LLMAGAMIGEE229 pKa = 4.53GTSYY233 pKa = 11.22PDD235 pKa = 2.94LTYY238 pKa = 10.34TVQLNSEE245 pKa = 4.34EE246 pKa = 4.33YY247 pKa = 10.71GVGFRR252 pKa = 11.84KK253 pKa = 10.53GSDD256 pKa = 3.07LVDD259 pKa = 3.54AFNTFWQEE267 pKa = 3.28AYY269 pKa = 10.65ADD271 pKa = 3.89GTVTEE276 pKa = 4.32VATTYY281 pKa = 10.66GVQEE285 pKa = 4.17SLIAYY290 pKa = 8.07

Molecular weight: 30.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y4T6B9|A0A1Y4T6B9_9FIRM Uncharacterized protein OS=Faecalibacterium sp. An121 OX=1965550 GN=B5E66_08035 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.6RR3 pKa = 11.84TFQPKK8 pKa = 8.94KK9 pKa = 7.92RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 6.43QVHH16 pKa = 5.62GFLTRR21 pKa = 11.84MSTKK25 pKa = 10.35NGRR28 pKa = 11.84KK29 pKa = 9.33VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.76GRR39 pKa = 11.84KK40 pKa = 8.97SLTVV44 pKa = 3.12

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2414

0

2414

781649

37

2752

323.8

35.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.673 ± 0.078

1.77 ± 0.021

5.596 ± 0.041

6.135 ± 0.053

3.663 ± 0.031

7.998 ± 0.047

1.8 ± 0.022

5.183 ± 0.038

4.256 ± 0.054

10.106 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.729 ± 0.023

3.132 ± 0.032

4.506 ± 0.034

4.082 ± 0.031

5.929 ± 0.052

5.355 ± 0.039

5.324 ± 0.039

7.033 ± 0.045

1.165 ± 0.018

3.563 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski