Staphylococcus virus 80alpha

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Azeredovirinae; Dubowvirus

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A4ZF92|A4ZF92_9CAUD Uncharacterized protein OS=Staphylococcus virus 80alpha OX=53369 PE=4 SV=1
MM1 pKa = 6.89QQQAYY6 pKa = 9.38INATIDD12 pKa = 2.95IRR14 pKa = 11.84IPTEE18 pKa = 3.49VEE20 pKa = 3.58YY21 pKa = 10.91QYY23 pKa = 11.49FDD25 pKa = 3.82DD26 pKa = 5.0VDD28 pKa = 3.64IEE30 pKa = 4.33KK31 pKa = 10.59EE32 pKa = 3.88ALADD36 pKa = 3.61YY37 pKa = 10.58LYY39 pKa = 11.17NNPDD43 pKa = 3.26EE44 pKa = 4.53LLEE47 pKa = 3.95YY48 pKa = 11.05DD49 pKa = 3.49NLKK52 pKa = 9.69IRR54 pKa = 11.84NVNVEE59 pKa = 3.93VEE61 pKa = 4.1

Molecular weight: 7.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A4ZFD9|A4ZFD9_9CAUD Uncharacterized protein OS=Staphylococcus virus 80alpha OX=53369 PE=4 SV=1
MM1 pKa = 7.85LLRR4 pKa = 11.84YY5 pKa = 8.32MYY7 pKa = 10.73RR8 pKa = 11.84VMTRR12 pKa = 11.84KK13 pKa = 8.83MSPVGPTVRR22 pKa = 11.84YY23 pKa = 6.59MASARR28 pKa = 11.84LYY30 pKa = 11.11SSLLLYY36 pKa = 10.07KK37 pKa = 10.53SNYY40 pKa = 8.75NIKK43 pKa = 10.44APRR46 pKa = 11.84KK47 pKa = 9.22LLYY50 pKa = 10.96GNAKK54 pKa = 9.98VIYY57 pKa = 6.98TTGSSMKK64 pKa = 9.88TMLSIARR71 pKa = 11.84KK72 pKa = 9.66NIQQ75 pKa = 2.94

Molecular weight: 8.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

14103

42

1154

193.2

22.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.488 ± 0.468

0.617 ± 0.089

6.24 ± 0.336

7.552 ± 0.504

4.233 ± 0.197

5.417 ± 0.306

1.709 ± 0.153

7.516 ± 0.252

9.317 ± 0.391

7.622 ± 0.307

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.659 ± 0.157

7.013 ± 0.272

2.716 ± 0.193

3.652 ± 0.169

4.184 ± 0.229

5.878 ± 0.205

6.204 ± 0.251

6.226 ± 0.275

1.085 ± 0.156

4.673 ± 0.378
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski