Faecalibacterium phage FP_oengus
Average proteome isoelectric point is 6.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2K9V395|A0A2K9V395_9CAUD Uncharacterized protein OS=Faecalibacterium phage FP_oengus OX=2070188 PE=4 SV=1
MM1 pKa = 7.53 SARR4 pKa = 11.84 NYY6 pKa = 9.08 VPAMVKK12 pKa = 9.74 WMVEE16 pKa = 3.91 EE17 pKa = 4.67 GTKK20 pKa = 8.92 NTSSGNWIFTSAEE33 pKa = 3.61 IAEE36 pKa = 4.55 AFPVAEE42 pKa = 4.15 SSVIEE47 pKa = 4.18 MFGAILTEE55 pKa = 4.77 VYY57 pKa = 9.59 QHH59 pKa = 6.24 EE60 pKa = 4.62 AVAEE64 pKa = 4.08 ANVNFEE70 pKa = 4.52 SDD72 pKa = 3.1 GSATFDD78 pKa = 3.05 LTFYY82 pKa = 10.89 TDD84 pKa = 3.64 YY85 pKa = 10.86 CPNISDD91 pKa = 3.61 EE92 pKa = 4.51 TKK94 pKa = 10.69 AGG96 pKa = 3.49
Molecular weight: 10.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.852
IPC2_protein 4.101
IPC_protein 3.961
Toseland 3.808
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.062
Wikipedia 3.77
Rodwell 3.795
Grimsley 3.732
Solomon 3.884
Lehninger 3.834
Nozaki 4.037
DTASelect 4.113
Thurlkill 3.834
EMBOSS 3.795
Sillero 4.062
Patrickios 1.888
IPC_peptide 3.897
IPC2_peptide 4.05
IPC2.peptide.svr19 3.963
Protein with the highest isoelectric point:
>tr|A0A2K9V3B3|A0A2K9V3B3_9CAUD Uncharacterized protein OS=Faecalibacterium phage FP_oengus OX=2070188 PE=4 SV=1
MM1 pKa = 7.22 QNLSKK6 pKa = 10.79 KK7 pKa = 9.55 HH8 pKa = 5.7 LKK10 pKa = 9.66 QIYY13 pKa = 8.96 RR14 pKa = 11.84 RR15 pKa = 11.84 RR16 pKa = 11.84 NGFGGATVMLSKK28 pKa = 10.26 FFRR31 pKa = 11.84 AAPNNRR37 pKa = 11.84 ADD39 pKa = 3.99 YY40 pKa = 11.04 NKK42 pKa = 9.88 MMDD45 pKa = 4.26 WRR47 pKa = 11.84 WSMCTNVRR55 pKa = 11.84 YY56 pKa = 8.0 MIPGEE61 pKa = 4.15 KK62 pKa = 10.55 LNGAKK67 pKa = 10.21 KK68 pKa = 10.41
Molecular weight: 8.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.301
IPC2_protein 10.072
IPC_protein 10.847
Toseland 11.052
ProMoST 10.804
Dawson 11.125
Bjellqvist 10.833
Wikipedia 11.33
Rodwell 11.462
Grimsley 11.155
Solomon 11.272
Lehninger 11.228
Nozaki 11.023
DTASelect 10.818
Thurlkill 11.038
EMBOSS 11.462
Sillero 11.052
Patrickios 11.199
IPC_peptide 11.272
IPC2_peptide 9.765
IPC2.peptide.svr19 8.218
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
84
0
84
17183
29
2202
204.6
22.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.794 ± 0.594
1.513 ± 0.209
6.367 ± 0.288
7.35 ± 0.266
3.469 ± 0.188
6.658 ± 0.423
1.699 ± 0.214
5.808 ± 0.222
7.496 ± 0.265
8.159 ± 0.396
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.178 ± 0.161
4.859 ± 0.141
3.148 ± 0.282
3.125 ± 0.164
4.603 ± 0.441
5.593 ± 0.385
6.192 ± 0.343
6.297 ± 0.194
1.507 ± 0.105
4.184 ± 0.372
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here