Pteropus associated gemycircularvirus 10
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A140CTL6|A0A140CTL6_9VIRU RepA OS=Pteropus associated gemycircularvirus 10 OX=1985404 PE=3 SV=1
MM1 pKa = 7.44 TFHH4 pKa = 7.09 FSARR8 pKa = 11.84 YY9 pKa = 9.48 VLLTYY14 pKa = 10.06 AQSGDD19 pKa = 3.41 LSEE22 pKa = 4.41 WSVLDD27 pKa = 4.62 HH28 pKa = 6.79 ISSLGAEE35 pKa = 4.41 CIVARR40 pKa = 11.84 EE41 pKa = 4.04 DD42 pKa = 3.46 HH43 pKa = 6.99 ADD45 pKa = 3.7 GGTHH49 pKa = 6.54 LHH51 pKa = 5.76 VFCDD55 pKa = 5.07 FGRR58 pKa = 11.84 KK59 pKa = 8.32 KK60 pKa = 10.15 QSRR63 pKa = 11.84 RR64 pKa = 11.84 PDD66 pKa = 3.36 YY67 pKa = 10.83 FDD69 pKa = 4.99 VEE71 pKa = 4.4 GHH73 pKa = 6.6 HH74 pKa = 6.71 PNIVPSRR81 pKa = 11.84 GRR83 pKa = 11.84 AGEE86 pKa = 4.02 GWDD89 pKa = 3.56 YY90 pKa = 10.72 ATKK93 pKa = 10.53 DD94 pKa = 3.54 GNIVAGGLEE103 pKa = 4.1 RR104 pKa = 11.84 PGGGGLPSTPDD115 pKa = 2.6 KK116 pKa = 10.39 WRR118 pKa = 11.84 EE119 pKa = 3.61 IVGAEE124 pKa = 3.82 SRR126 pKa = 11.84 EE127 pKa = 3.99 EE128 pKa = 4.19 FFDD131 pKa = 5.34 LLRR134 pKa = 11.84 QLDD137 pKa = 4.38 PKK139 pKa = 10.33 TLITRR144 pKa = 11.84 WSEE147 pKa = 3.6 LNRR150 pKa = 11.84 YY151 pKa = 9.13 ADD153 pKa = 3.12 AAYY156 pKa = 7.63 ATRR159 pKa = 11.84 AEE161 pKa = 4.79 PYY163 pKa = 9.97 VGPTGIEE170 pKa = 4.16 FEE172 pKa = 4.44 LGMVPEE178 pKa = 4.37 LARR181 pKa = 11.84 WGRR184 pKa = 11.84 EE185 pKa = 3.75 LPVLYY190 pKa = 10.58 GPSRR194 pKa = 11.84 LGKK197 pKa = 7.27 TLWARR202 pKa = 11.84 SLGPHH207 pKa = 6.96 AYY209 pKa = 9.55 IMGMVSGHH217 pKa = 5.28 VLTRR221 pKa = 11.84 DD222 pKa = 3.44 MPDD225 pKa = 2.6 AQYY228 pKa = 11.73 AVFDD232 pKa = 4.43 DD233 pKa = 3.82 MRR235 pKa = 11.84 GGISMFPSFKK245 pKa = 10.24 EE246 pKa = 3.57 WFGAQEE252 pKa = 4.17 VVTVKK257 pKa = 9.72 TLYY260 pKa = 10.24 RR261 pKa = 11.84 DD262 pKa = 3.61 PVQMKK267 pKa = 8.97 WGKK270 pKa = 8.84 PCIWLANADD279 pKa = 3.96 PRR281 pKa = 11.84 DD282 pKa = 3.7 QLKK285 pKa = 10.93 ADD287 pKa = 3.28 ITDD290 pKa = 3.66 RR291 pKa = 11.84 TPKK294 pKa = 10.53 GRR296 pKa = 11.84 IDD298 pKa = 5.41 LIYY301 pKa = 10.94 NDD303 pKa = 4.47 IEE305 pKa = 4.1 WMEE308 pKa = 4.19 ANCIFVGLQEE318 pKa = 4.88 AIFRR322 pKa = 11.84 ASTEE326 pKa = 3.81
Molecular weight: 36.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.367
IPC2_protein 5.359
IPC_protein 5.347
Toseland 5.537
ProMoST 5.486
Dawson 5.461
Bjellqvist 5.55
Wikipedia 5.41
Rodwell 5.41
Grimsley 5.588
Solomon 5.461
Lehninger 5.436
Nozaki 5.652
DTASelect 5.842
Thurlkill 5.664
EMBOSS 5.626
Sillero 5.741
Patrickios 4.139
IPC_peptide 5.474
IPC2_peptide 5.753
IPC2.peptide.svr19 5.702
Protein with the highest isoelectric point:
>tr|A0A140CTL5|A0A140CTL5_9VIRU Replication-associated protein OS=Pteropus associated gemycircularvirus 10 OX=1985404 PE=3 SV=1
MM1 pKa = 7.38 TLHH4 pKa = 6.55 SLAARR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 YY12 pKa = 9.59 AKK14 pKa = 10.66 LMASRR19 pKa = 11.84 RR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 VPAGRR27 pKa = 11.84 RR28 pKa = 11.84 SRR30 pKa = 11.84 PRR32 pKa = 11.84 SRR34 pKa = 11.84 YY35 pKa = 5.02 TAKK38 pKa = 9.56 TRR40 pKa = 11.84 RR41 pKa = 11.84 YY42 pKa = 8.55 APKK45 pKa = 10.12 RR46 pKa = 11.84 PMSKK50 pKa = 10.14 KK51 pKa = 10.3 RR52 pKa = 11.84 ILDD55 pKa = 3.21 VTTVKK60 pKa = 10.51 KK61 pKa = 10.16 RR62 pKa = 11.84 DD63 pKa = 3.51 NMPCYY68 pKa = 10.68 TNMTNSGAGTTFSITAAYY86 pKa = 8.85 IRR88 pKa = 11.84 PNTSSDD94 pKa = 3.39 PLINHH99 pKa = 5.99 PTVMLWNATARR110 pKa = 11.84 DD111 pKa = 3.98 LTNSAGTINIRR122 pKa = 11.84 AVEE125 pKa = 4.16 SARR128 pKa = 11.84 TSSTPYY134 pKa = 9.78 MVGLKK139 pKa = 8.84 EE140 pKa = 4.09 TVEE143 pKa = 4.28 IQASSGMPWQWRR155 pKa = 11.84 RR156 pKa = 11.84 ICFTYY161 pKa = 10.34 KK162 pKa = 10.72 GILPGSTPTTTYY174 pKa = 9.73 YY175 pKa = 10.25 PYY177 pKa = 10.58 RR178 pKa = 11.84 EE179 pKa = 4.08 TSSGFVRR186 pKa = 11.84 VASPLSGDD194 pKa = 3.65 RR195 pKa = 11.84 NSGATYY201 pKa = 10.99 DD202 pKa = 3.68 LFYY205 pKa = 10.98 LIFAGQNASDD215 pKa = 3.47 WTDD218 pKa = 3.33 PMVAKK223 pKa = 10.57 LDD225 pKa = 3.52 QTRR228 pKa = 11.84 ISVKK232 pKa = 9.58 YY233 pKa = 10.28 DD234 pKa = 2.92 KK235 pKa = 10.89 TRR237 pKa = 11.84 TLSCGNDD244 pKa = 3.26 DD245 pKa = 4.37 GSIRR249 pKa = 11.84 KK250 pKa = 8.5 YY251 pKa = 10.53 KK252 pKa = 9.66 HH253 pKa = 3.45 WHH255 pKa = 5.77 AMGKK259 pKa = 7.26 TLAYY263 pKa = 10.47 DD264 pKa = 3.78 DD265 pKa = 5.27 DD266 pKa = 4.91 EE267 pKa = 6.14 NGEE270 pKa = 4.14 NMITNYY276 pKa = 10.25 QSVDD280 pKa = 3.16 SRR282 pKa = 11.84 VGMGDD287 pKa = 3.72 YY288 pKa = 10.87 YY289 pKa = 11.55 VVDD292 pKa = 4.2 LFRR295 pKa = 11.84 SRR297 pKa = 11.84 FGATASDD304 pKa = 3.46 SLLFNPAATLYY315 pKa = 9.05 WHH317 pKa = 7.05 EE318 pKa = 4.35 KK319 pKa = 9.3
Molecular weight: 36.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.125
IPC2_protein 9.633
IPC_protein 10.101
Toseland 9.999
ProMoST 9.882
Dawson 10.277
Bjellqvist 10.028
Wikipedia 10.511
Rodwell 10.496
Grimsley 10.379
Solomon 10.306
Lehninger 10.262
Nozaki 10.014
DTASelect 10.014
Thurlkill 10.101
EMBOSS 10.438
Sillero 10.189
Patrickios 7.571
IPC_peptide 10.306
IPC2_peptide 8.902
IPC2.peptide.svr19 8.697
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
886
241
326
295.3
33.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.126 ± 0.139
1.354 ± 0.263
6.433 ± 0.423
5.192 ± 1.225
3.612 ± 0.453
8.804 ± 1.206
2.483 ± 0.396
4.176 ± 0.385
3.837 ± 0.557
7.675 ± 0.793
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.822 ± 0.464
3.047 ± 0.695
5.418 ± 0.196
1.919 ± 0.24
8.691 ± 0.57
7.111 ± 1.001
6.772 ± 1.46
5.643 ± 0.429
2.483 ± 0.284
4.402 ± 0.64
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here