Muromegalovirus G4
Average proteome isoelectric point is 7.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 163 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B3UX83|B3UX83_MUHV1 M138 OS=Muromegalovirus G4 OX=524650 GN=m138 PE=4 SV=1
MM1 pKa = 7.92 AINTTEE7 pKa = 4.23 CPSPSEE13 pKa = 4.15 TVSAQTIVLTCAALTSVLLAAAILVVAISALVRR46 pKa = 11.84 RR47 pKa = 11.84 CRR49 pKa = 11.84 RR50 pKa = 11.84 QQTPRR55 pKa = 11.84 GDD57 pKa = 3.38 EE58 pKa = 3.82 QLPIEE63 pKa = 4.42 CSVYY67 pKa = 10.08 LVQNNGVDD75 pKa = 4.75 DD76 pKa = 5.38 DD77 pKa = 5.51 DD78 pKa = 4.77 IPPGFLGRR86 pKa = 11.84 PAGYY90 pKa = 10.42 EE91 pKa = 3.8 DD92 pKa = 4.46 MSPHH96 pKa = 6.63 SFPLDD101 pKa = 3.9 EE102 pKa = 5.83 IYY104 pKa = 11.02 EE105 pKa = 4.53 SIDD108 pKa = 3.11
Molecular weight: 11.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.874
IPC2_protein 4.164
IPC_protein 4.062
Toseland 3.872
ProMoST 4.126
Dawson 4.037
Bjellqvist 4.253
Wikipedia 3.961
Rodwell 3.897
Grimsley 3.783
Solomon 4.024
Lehninger 3.986
Nozaki 4.164
DTASelect 4.342
Thurlkill 3.923
EMBOSS 3.973
Sillero 4.177
Patrickios 1.952
IPC_peptide 4.024
IPC2_peptide 4.164
IPC2.peptide.svr19 4.1
Protein with the highest isoelectric point:
>tr|B3UWX9|B3UWX9_MUHV1 M26 OS=Muromegalovirus G4 OX=524650 GN=M26 PE=4 SV=1
MM1 pKa = 7.61 PASSCTSRR9 pKa = 11.84 TTSRR13 pKa = 11.84 SSSRR17 pKa = 11.84 TASLEE22 pKa = 3.79 GTSRR26 pKa = 11.84 YY27 pKa = 9.99 SATCVRR33 pKa = 11.84 ASYY36 pKa = 10.27 RR37 pKa = 11.84 RR38 pKa = 11.84 SSRR41 pKa = 11.84 SASATSRR48 pKa = 11.84 VLPGYY53 pKa = 9.6 RR54 pKa = 11.84 RR55 pKa = 11.84 PSASGCPSSSEE66 pKa = 4.53 AYY68 pKa = 9.32 SAPGSRR74 pKa = 11.84 PRR76 pKa = 11.84 GSAGRR81 pKa = 11.84 SDD83 pKa = 3.64 SVPRR87 pKa = 11.84 IFRR90 pKa = 11.84 TGCLRR95 pKa = 11.84 RR96 pKa = 11.84 PWLCPTCEE104 pKa = 3.81 RR105 pKa = 11.84 RR106 pKa = 11.84 RR107 pKa = 11.84 RR108 pKa = 11.84 RR109 pKa = 11.84 PPLRR113 pKa = 11.84 PWRR116 pKa = 11.84 PQRR119 pKa = 11.84 RR120 pKa = 11.84 SDD122 pKa = 3.08 TVARR126 pKa = 11.84 KK127 pKa = 7.55 RR128 pKa = 11.84 TRR130 pKa = 11.84 SRR132 pKa = 11.84 TRR134 pKa = 11.84 TRR136 pKa = 11.84 TRR138 pKa = 11.84 IRR140 pKa = 11.84 NGAKK144 pKa = 10.04 GDD146 pKa = 3.69 QTMLAPAAAVSMPEE160 pKa = 4.16 SEE162 pKa = 4.4 TEE164 pKa = 4.03 MVTVVPARR172 pKa = 11.84 STLAAKK178 pKa = 9.64 AAAMRR183 pKa = 11.84 KK184 pKa = 9.08 AYY186 pKa = 10.05 AVNRR190 pKa = 11.84 LLALGRR196 pKa = 11.84 AAAEE200 pKa = 4.4 GKK202 pKa = 10.26 LEE204 pKa = 3.88 QWLSARR210 pKa = 11.84 RR211 pKa = 11.84 GEE213 pKa = 4.48 RR214 pKa = 11.84 IPLVFPEE221 pKa = 4.44 KK222 pKa = 9.78 CAKK225 pKa = 10.26 YY226 pKa = 10.48 LILCAPGDD234 pKa = 4.08 VDD236 pKa = 5.18 GHH238 pKa = 6.02 EE239 pKa = 5.07 CDD241 pKa = 4.15 PDD243 pKa = 3.47 ALRR246 pKa = 11.84 ALEE249 pKa = 4.12 PAVDD253 pKa = 3.61 RR254 pKa = 11.84 LVVVGFVEE262 pKa = 4.37 GRR264 pKa = 11.84 EE265 pKa = 3.97 DD266 pKa = 3.18 AHH268 pKa = 8.0 RR269 pKa = 11.84 DD270 pKa = 3.14 GDD272 pKa = 3.9 DD273 pKa = 3.28 RR274 pKa = 11.84 CIVLVNPSSAVFIYY288 pKa = 10.67 DD289 pKa = 3.59 PAPYY293 pKa = 10.2 GGLYY297 pKa = 10.35 RR298 pKa = 11.84 LANTMIGFVRR308 pKa = 11.84 RR309 pKa = 11.84 GLRR312 pKa = 11.84 RR313 pKa = 11.84 FDD315 pKa = 3.68 SIYY318 pKa = 10.42 RR319 pKa = 11.84 DD320 pKa = 3.69 PCCVEE325 pKa = 3.52 DD326 pKa = 5.17 AIRR329 pKa = 11.84 VKK331 pKa = 10.62 DD332 pKa = 3.59 GHH334 pKa = 6.96 ASLPRR339 pKa = 11.84 NADD342 pKa = 3.23 EE343 pKa = 4.95 ALAAAEE349 pKa = 4.49 RR350 pKa = 11.84 GLACTLQWPEE360 pKa = 4.61 DD361 pKa = 3.77 YY362 pKa = 11.05 DD363 pKa = 4.35 FVFGTPPEE371 pKa = 4.23 SSNAVASSLNKK382 pKa = 9.6 EE383 pKa = 3.83 QSPCIDD389 pKa = 3.64 ALRR392 pKa = 11.84 LSTFGHH398 pKa = 6.75 FGTVAGLSSTRR409 pKa = 11.84 PRR411 pKa = 11.84 TAVFIGADD419 pKa = 3.27 HH420 pKa = 6.65 AVYY423 pKa = 10.15 AIHH426 pKa = 6.75 HH427 pKa = 6.31 AVSKK431 pKa = 10.27 LLRR434 pKa = 11.84 LAEE437 pKa = 4.32 SLDD440 pKa = 3.45 MFYY443 pKa = 10.9 KK444 pKa = 10.3 IGIRR448 pKa = 11.84 RR449 pKa = 11.84 FFHH452 pKa = 6.75 NYY454 pKa = 8.12 RR455 pKa = 11.84 VVPSRR460 pKa = 11.84 CGDD463 pKa = 3.13 DD464 pKa = 4.07 RR465 pKa = 11.84 FFLKK469 pKa = 10.65 AVV471 pKa = 3.35
Molecular weight: 51.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.332
IPC2_protein 9.399
IPC_protein 10.189
Toseland 10.101
ProMoST 9.97
Dawson 10.335
Bjellqvist 10.145
Wikipedia 10.584
Rodwell 10.394
Grimsley 10.423
Solomon 10.409
Lehninger 10.35
Nozaki 10.233
DTASelect 10.101
Thurlkill 10.189
EMBOSS 10.54
Sillero 10.292
Patrickios 9.619
IPC_peptide 10.394
IPC2_peptide 9.516
IPC2.peptide.svr19 8.405
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
163
0
163
65346
57
2149
400.9
44.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.036 ± 0.171
2.207 ± 0.094
5.893 ± 0.146
5.356 ± 0.142
3.791 ± 0.114
6.635 ± 0.225
2.208 ± 0.098
4.358 ± 0.115
3.506 ± 0.133
8.524 ± 0.181
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.198 ± 0.074
3.298 ± 0.09
6.034 ± 0.204
2.911 ± 0.105
8.118 ± 0.223
8.565 ± 0.242
6.894 ± 0.241
7.428 ± 0.159
1.065 ± 0.064
2.975 ± 0.099
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here