Shahe narna-like virus 5
Average proteome isoelectric point is 8.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KI92|A0A1L3KI92_9VIRU RNA-dependent RNA polymerase OS=Shahe narna-like virus 5 OX=1923433 PE=4 SV=1
MM1 pKa = 7.8 KK2 pKa = 9.57 MFKK5 pKa = 10.59 YY6 pKa = 10.01 MRR8 pKa = 11.84 GDD10 pKa = 3.56 ATWDD14 pKa = 3.1 QEE16 pKa = 4.13 KK17 pKa = 9.88 QVRR20 pKa = 11.84 RR21 pKa = 11.84 ICKK24 pKa = 6.41 WTEE27 pKa = 3.32 RR28 pKa = 11.84 GMKK31 pKa = 9.99 LYY33 pKa = 11.02 SFDD36 pKa = 3.35 LTAATDD42 pKa = 3.74 RR43 pKa = 11.84 WPAWHH48 pKa = 6.37 QKK50 pKa = 10.33 LVVDD54 pKa = 4.28 RR55 pKa = 11.84 VFNKK59 pKa = 10.8 GIGEE63 pKa = 3.87 MWFRR67 pKa = 11.84 LMTTTTPYY75 pKa = 9.35 ATEE78 pKa = 4.05 LKK80 pKa = 10.28 RR81 pKa = 11.84 YY82 pKa = 8.36 IRR84 pKa = 11.84 YY85 pKa = 9.86 AIGQPMGAYY94 pKa = 10.19 SSWAVLNMTHH104 pKa = 6.87 HH105 pKa = 5.5 YY106 pKa = 10.15 VIRR109 pKa = 11.84 LCAKK113 pKa = 10.16 RR114 pKa = 11.84 IGVKK118 pKa = 10.16 ALYY121 pKa = 10.13 SVLGDD126 pKa = 3.51 DD127 pKa = 3.85 VVIVGKK133 pKa = 10.11 EE134 pKa = 3.5 LADD137 pKa = 3.85 EE138 pKa = 4.1 YY139 pKa = 10.96 RR140 pKa = 11.84 SYY142 pKa = 9.78 ITRR145 pKa = 11.84 LGVKK149 pKa = 10.26 LNLNKK154 pKa = 10.56 SILNTDD160 pKa = 4.88 FGPCSAEE167 pKa = 3.9 FARR170 pKa = 11.84 HH171 pKa = 4.76 IVRR174 pKa = 11.84 DD175 pKa = 3.47 GHH177 pKa = 6.84 IVGTFSPSLVRR188 pKa = 11.84 NIYY191 pKa = 10.53 SYY193 pKa = 10.33 TDD195 pKa = 3.19 VYY197 pKa = 11.69 ASIDD201 pKa = 3.63 LLRR204 pKa = 11.84 EE205 pKa = 4.12 VQSKK209 pKa = 10.08 LGQVIHH215 pKa = 6.84 VYY217 pKa = 10.81 EE218 pKa = 4.39 NTTLLPIPIVRR229 pKa = 11.84 LLKK232 pKa = 9.98 TFSFKK237 pKa = 10.93 DD238 pKa = 3.33 DD239 pKa = 3.61 LLAIVTVPLTALKK252 pKa = 10.38 LPRR255 pKa = 11.84 VYY257 pKa = 10.47 HH258 pKa = 5.59 PEE260 pKa = 3.92 KK261 pKa = 10.9 FEE263 pKa = 4.25 GDD265 pKa = 3.51 PMVGYY270 pKa = 10.73 DD271 pKa = 3.52 KK272 pKa = 10.83 FANPWYY278 pKa = 9.47 EE279 pKa = 3.73 CDD281 pKa = 3.42 INSVNAEE288 pKa = 4.28 FGNKK292 pKa = 7.03 WMRR295 pKa = 11.84 EE296 pKa = 3.4 ASLRR300 pKa = 11.84 MNKK303 pKa = 10.02 LEE305 pKa = 4.32 SLLDD309 pKa = 3.57 KK310 pKa = 11.24 LGDD313 pKa = 4.01 GGSTVYY319 pKa = 10.32 RR320 pKa = 11.84 GRR322 pKa = 11.84 LLEE325 pKa = 4.32 ISSHH329 pKa = 5.11 PLRR332 pKa = 11.84 YY333 pKa = 9.47 SLKK336 pKa = 8.63 PLKK339 pKa = 10.23 EE340 pKa = 3.8 EE341 pKa = 5.21 LMNQFWRR348 pKa = 11.84 VMRR351 pKa = 11.84 GEE353 pKa = 4.1 EE354 pKa = 4.05 VPIFSSQLQTDD365 pKa = 3.22 IDD367 pKa = 4.22 LLIFTLTEE375 pKa = 4.08 GKK377 pKa = 9.91 SFRR380 pKa = 11.84 TWRR383 pKa = 11.84 NSKK386 pKa = 7.86 TSKK389 pKa = 10.14 RR390 pKa = 11.84 KK391 pKa = 8.1 HH392 pKa = 5.21 ACMLIKK398 pKa = 10.35 KK399 pKa = 8.04 VLKK402 pKa = 9.81 EE403 pKa = 3.91 SHH405 pKa = 6.03 RR406 pKa = 11.84 VAAAMKK412 pKa = 8.36 EE413 pKa = 4.09 LPTTSMSWEE422 pKa = 4.37 DD423 pKa = 3.46 YY424 pKa = 10.98 AKK426 pKa = 10.51 QFEE429 pKa = 4.31
Molecular weight: 49.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.818
IPC2_protein 8.843
IPC_protein 8.741
Toseland 9.385
ProMoST 9.209
Dawson 9.692
Bjellqvist 9.428
Wikipedia 9.882
Rodwell 9.94
Grimsley 9.78
Solomon 9.721
Lehninger 9.677
Nozaki 9.502
DTASelect 9.399
Thurlkill 9.531
EMBOSS 9.838
Sillero 9.633
Patrickios 4.838
IPC_peptide 9.706
IPC2_peptide 8.039
IPC2.peptide.svr19 7.821
Protein with the highest isoelectric point:
>tr|A0A1L3KI92|A0A1L3KI92_9VIRU RNA-dependent RNA polymerase OS=Shahe narna-like virus 5 OX=1923433 PE=4 SV=1
MM1 pKa = 7.8 KK2 pKa = 9.57 MFKK5 pKa = 10.59 YY6 pKa = 10.01 MRR8 pKa = 11.84 GDD10 pKa = 3.56 ATWDD14 pKa = 3.1 QEE16 pKa = 4.13 KK17 pKa = 9.88 QVRR20 pKa = 11.84 RR21 pKa = 11.84 ICKK24 pKa = 6.41 WTEE27 pKa = 3.32 RR28 pKa = 11.84 GMKK31 pKa = 9.99 LYY33 pKa = 11.02 SFDD36 pKa = 3.35 LTAATDD42 pKa = 3.74 RR43 pKa = 11.84 WPAWHH48 pKa = 6.37 QKK50 pKa = 10.33 LVVDD54 pKa = 4.28 RR55 pKa = 11.84 VFNKK59 pKa = 10.8 GIGEE63 pKa = 3.87 MWFRR67 pKa = 11.84 LMTTTTPYY75 pKa = 9.35 ATEE78 pKa = 4.05 LKK80 pKa = 10.28 RR81 pKa = 11.84 YY82 pKa = 8.36 IRR84 pKa = 11.84 YY85 pKa = 9.86 AIGQPMGAYY94 pKa = 10.19 SSWAVLNMTHH104 pKa = 6.87 HH105 pKa = 5.5 YY106 pKa = 10.15 VIRR109 pKa = 11.84 LCAKK113 pKa = 10.16 RR114 pKa = 11.84 IGVKK118 pKa = 10.16 ALYY121 pKa = 10.13 SVLGDD126 pKa = 3.51 DD127 pKa = 3.85 VVIVGKK133 pKa = 10.11 EE134 pKa = 3.5 LADD137 pKa = 3.85 EE138 pKa = 4.1 YY139 pKa = 10.96 RR140 pKa = 11.84 SYY142 pKa = 9.78 ITRR145 pKa = 11.84 LGVKK149 pKa = 10.26 LNLNKK154 pKa = 10.56 SILNTDD160 pKa = 4.88 FGPCSAEE167 pKa = 3.9 FARR170 pKa = 11.84 HH171 pKa = 4.76 IVRR174 pKa = 11.84 DD175 pKa = 3.47 GHH177 pKa = 6.84 IVGTFSPSLVRR188 pKa = 11.84 NIYY191 pKa = 10.53 SYY193 pKa = 10.33 TDD195 pKa = 3.19 VYY197 pKa = 11.69 ASIDD201 pKa = 3.63 LLRR204 pKa = 11.84 EE205 pKa = 4.12 VQSKK209 pKa = 10.08 LGQVIHH215 pKa = 6.84 VYY217 pKa = 10.81 EE218 pKa = 4.39 NTTLLPIPIVRR229 pKa = 11.84 LLKK232 pKa = 9.98 TFSFKK237 pKa = 10.93 DD238 pKa = 3.33 DD239 pKa = 3.61 LLAIVTVPLTALKK252 pKa = 10.38 LPRR255 pKa = 11.84 VYY257 pKa = 10.47 HH258 pKa = 5.59 PEE260 pKa = 3.92 KK261 pKa = 10.9 FEE263 pKa = 4.25 GDD265 pKa = 3.51 PMVGYY270 pKa = 10.73 DD271 pKa = 3.52 KK272 pKa = 10.83 FANPWYY278 pKa = 9.47 EE279 pKa = 3.73 CDD281 pKa = 3.42 INSVNAEE288 pKa = 4.28 FGNKK292 pKa = 7.03 WMRR295 pKa = 11.84 EE296 pKa = 3.4 ASLRR300 pKa = 11.84 MNKK303 pKa = 10.02 LEE305 pKa = 4.32 SLLDD309 pKa = 3.57 KK310 pKa = 11.24 LGDD313 pKa = 4.01 GGSTVYY319 pKa = 10.32 RR320 pKa = 11.84 GRR322 pKa = 11.84 LLEE325 pKa = 4.32 ISSHH329 pKa = 5.11 PLRR332 pKa = 11.84 YY333 pKa = 9.47 SLKK336 pKa = 8.63 PLKK339 pKa = 10.23 EE340 pKa = 3.8 EE341 pKa = 5.21 LMNQFWRR348 pKa = 11.84 VMRR351 pKa = 11.84 GEE353 pKa = 4.1 EE354 pKa = 4.05 VPIFSSQLQTDD365 pKa = 3.22 IDD367 pKa = 4.22 LLIFTLTEE375 pKa = 4.08 GKK377 pKa = 9.91 SFRR380 pKa = 11.84 TWRR383 pKa = 11.84 NSKK386 pKa = 7.86 TSKK389 pKa = 10.14 RR390 pKa = 11.84 KK391 pKa = 8.1 HH392 pKa = 5.21 ACMLIKK398 pKa = 10.35 KK399 pKa = 8.04 VLKK402 pKa = 9.81 EE403 pKa = 3.91 SHH405 pKa = 6.03 RR406 pKa = 11.84 VAAAMKK412 pKa = 8.36 EE413 pKa = 4.09 LPTTSMSWEE422 pKa = 4.37 DD423 pKa = 3.46 YY424 pKa = 10.98 AKK426 pKa = 10.51 QFEE429 pKa = 4.31
Molecular weight: 49.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.818
IPC2_protein 8.843
IPC_protein 8.741
Toseland 9.385
ProMoST 9.209
Dawson 9.692
Bjellqvist 9.428
Wikipedia 9.882
Rodwell 9.94
Grimsley 9.78
Solomon 9.721
Lehninger 9.677
Nozaki 9.502
DTASelect 9.399
Thurlkill 9.531
EMBOSS 9.838
Sillero 9.633
Patrickios 4.838
IPC_peptide 9.706
IPC2_peptide 8.039
IPC2.peptide.svr19 7.821
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
429
429
429
429.0
49.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.594 ± 0.0
1.166 ± 0.0
5.128 ± 0.0
5.828 ± 0.0
3.963 ± 0.0
5.361 ± 0.0
2.331 ± 0.0
5.361 ± 0.0
7.692 ± 0.0
10.49 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.73 ± 0.0
3.263 ± 0.0
3.73 ± 0.0
2.331 ± 0.0
6.993 ± 0.0
6.527 ± 0.0
6.294 ± 0.0
6.993 ± 0.0
2.564 ± 0.0
4.662 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here