Brachybacterium vulturis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Dermabacteraceae; Brachybacterium

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3244 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A291GLB2|A0A291GLB2_9MICO 30S ribosomal protein S5 OS=Brachybacterium vulturis OX=2017484 GN=rpsE PE=3 SV=1
MM1 pKa = 7.67TIHH4 pKa = 5.59STRR7 pKa = 11.84RR8 pKa = 11.84HH9 pKa = 5.22LLGALGAGSLALGAAACGSSDD30 pKa = 3.44PFDD33 pKa = 3.86EE34 pKa = 5.3GGEE37 pKa = 4.2EE38 pKa = 4.53GGGASDD44 pKa = 4.12GGSGAGTLAIGSQAYY59 pKa = 9.52YY60 pKa = 10.23SNEE63 pKa = 3.79IIAEE67 pKa = 4.1LCAQVLEE74 pKa = 4.66AEE76 pKa = 4.74GFTVDD81 pKa = 2.94RR82 pKa = 11.84QYY84 pKa = 11.36QIGQRR89 pKa = 11.84EE90 pKa = 4.46VYY92 pKa = 9.58MPEE95 pKa = 4.31LEE97 pKa = 4.39NGSLDD102 pKa = 4.75LLPEE106 pKa = 3.99YY107 pKa = 10.52LGNLLQYY114 pKa = 10.37YY115 pKa = 10.36DD116 pKa = 4.32SEE118 pKa = 5.27APAASPEE125 pKa = 4.31EE126 pKa = 3.86IHH128 pKa = 6.87GALAEE133 pKa = 4.24ALPEE137 pKa = 4.06GLRR140 pKa = 11.84VLDD143 pKa = 3.97FAEE146 pKa = 5.77ASDD149 pKa = 3.55QDD151 pKa = 4.36SYY153 pKa = 9.65TTTSDD158 pKa = 3.3FASTHH163 pKa = 5.74SLTTIGDD170 pKa = 3.88LAGVDD175 pKa = 3.94EE176 pKa = 4.95DD177 pKa = 5.37LKK179 pKa = 10.95IAANSEE185 pKa = 4.43FEE187 pKa = 4.09VRR189 pKa = 11.84PYY191 pKa = 10.88GPEE194 pKa = 3.85GAKK197 pKa = 10.07EE198 pKa = 3.89IYY200 pKa = 10.32GVDD203 pKa = 3.12ISVVPVEE210 pKa = 4.9DD211 pKa = 3.13SGGPLTVQALLDD223 pKa = 4.16GDD225 pKa = 4.02VQLADD230 pKa = 3.98IYY232 pKa = 10.08TANPAIAEE240 pKa = 4.1HH241 pKa = 6.82DD242 pKa = 4.14LVVLEE247 pKa = 5.31DD248 pKa = 4.41PEE250 pKa = 5.99SMILPQNVVPVVTEE264 pKa = 4.72KK265 pKa = 10.24IDD267 pKa = 3.49EE268 pKa = 4.19TAAAAINGVMAQLSADD284 pKa = 4.02DD285 pKa = 4.55LVEE288 pKa = 4.4LNRR291 pKa = 11.84QSVVDD296 pKa = 3.84QASSADD302 pKa = 3.62IATGWLTEE310 pKa = 3.99KK311 pKa = 11.06GLLL314 pKa = 3.98

Molecular weight: 32.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A291GKB0|A0A291GKB0_9MICO AraC family transcriptional regulator OS=Brachybacterium vulturis OX=2017484 GN=CFK38_03215 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 8.98RR4 pKa = 11.84TFQPNIRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.8KK16 pKa = 8.97HH17 pKa = 4.4GFRR20 pKa = 11.84ARR22 pKa = 11.84MSTRR26 pKa = 11.84AGRR29 pKa = 11.84AILNARR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.65GRR40 pKa = 11.84AALSAA45 pKa = 3.96

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3244

0

3244

1105779

32

2076

340.9

36.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.062 ± 0.057

0.566 ± 0.009

6.045 ± 0.034

6.371 ± 0.036

2.8 ± 0.026

9.149 ± 0.037

2.312 ± 0.019

4.144 ± 0.031

1.605 ± 0.028

10.703 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.006 ± 0.017

1.602 ± 0.021

5.761 ± 0.034

3.026 ± 0.018

7.645 ± 0.043

5.625 ± 0.026

6.151 ± 0.026

8.274 ± 0.03

1.405 ± 0.019

1.747 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski