Streptomyces phage Salete

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Woodruffvirus; unclassified Woodruffvirus

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U8UWP3|A0A2U8UWP3_9CAUD Uncharacterized protein OS=Streptomyces phage Salete OX=2182343 GN=33 PE=4 SV=1
MM1 pKa = 8.05PDD3 pKa = 4.26DD4 pKa = 5.47LDD6 pKa = 3.7PTACVDD12 pKa = 3.46AEE14 pKa = 4.18GFEE17 pKa = 4.56WPEE20 pKa = 3.11HH21 pKa = 6.94DD22 pKa = 4.68YY23 pKa = 11.87GHH25 pKa = 6.42TEE27 pKa = 3.98CSRR30 pKa = 11.84CGAEE34 pKa = 4.54APP36 pKa = 3.97

Molecular weight: 3.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U8UWR6|A0A2U8UWR6_9CAUD Uncharacterized protein OS=Streptomyces phage Salete OX=2182343 GN=46 PE=4 SV=1
MM1 pKa = 7.28TATATATATATAAKK15 pKa = 9.98FEE17 pKa = 4.42TGDD20 pKa = 3.94VVWGRR25 pKa = 11.84PVRR28 pKa = 11.84QGKK31 pKa = 8.62AQARR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 10.34GLVLGLFATDD47 pKa = 3.9PNQLVVWWFGQGAAGMDD64 pKa = 3.54TTTLAFARR72 pKa = 11.84EE73 pKa = 4.17LTKK76 pKa = 11.08SGDD79 pKa = 3.62IFDD82 pKa = 4.28MGAVQAAKK90 pKa = 10.2LARR93 pKa = 11.84GCYY96 pKa = 9.22RR97 pKa = 11.84YY98 pKa = 10.14EE99 pKa = 3.99RR100 pKa = 11.84AHH102 pKa = 5.58SVGRR106 pKa = 11.84MLEE109 pKa = 3.82RR110 pKa = 11.84HH111 pKa = 5.28ARR113 pKa = 11.84RR114 pKa = 11.84MKK116 pKa = 10.74SLGASFPAA124 pKa = 4.73

Molecular weight: 13.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

17401

36

1197

245.1

26.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.712 ± 0.538

1.626 ± 0.254

6.471 ± 0.26

5.195 ± 0.22

2.707 ± 0.179

9.017 ± 0.389

1.942 ± 0.203

3.517 ± 0.208

2.988 ± 0.267

8.373 ± 0.305

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.253 ± 0.135

2.695 ± 0.168

5.689 ± 0.276

3.23 ± 0.106

6.89 ± 0.306

5.276 ± 0.181

6.862 ± 0.242

7.948 ± 0.195

1.638 ± 0.114

1.971 ± 0.163
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski